Biomedical subjects
Publications and source records attributed to H Kuno-Sakai.
Effects and side effects of a new measles-mumps-rubella (MMR) vaccine were described. After MMR vaccination, 98% (59/60) of children showed seroconversion by the measles HI test, 98% (65/66) showed seroconversion by the rubella HI test and 57% (34/60) showed seroconversion by mumps NT. Side effects of MMR vaccine were fever (5/82) and skin rash (3/82).
Immunoglobulin-bearing peripheral blood lymphocytes were examined in 20 children with Kawasaki disease and in 13 healthy control children. Routine laboratory tests were also performed. IgG-bearing lymphocytes, IgA-bearing lymphocytes, and IgM-bearing lymphocytes increased in children with Kawasaki disease when compared with those of controls. Measurement of immunoglobulin-bearing cells in peripheral blood may be used as a clinical test in the diagnosis of Kawasaki disease.
Aberration of IgA-bearing peripheral blood lymphocytes in children with Henoch-Schoenlein purpura has been investigated. Eighteen children with Henoch-Schoenlein purpura demonstrated a marked increase of IgA-bearing lymphocytes in peripheral blood during the acute phase of the disease. The levels of IgA-bearing lymphocytes returned to the normal levels after the acute symptoms of Henoch-Schoenlein purpura had subsided. However, in patients who developed purpura nephritis, the increased levels of IgA-bearing lymphocytes in peripheral blood were retained after the disappearance of purpura, and lasted for more than 12 months. It is concluded that the monitoring of IgA-bearing peripheral blood lymphocytes is useful for the screening of patients with Henoch-Schoenlein purpura and purpura nephritis.
Patients with acute thermal burns experience an increase of serum immunoglobulins associated with marked B-lymphocytosis during the recovery phase from the burn injury. This study was performed to delineate humoral factors which may induce the B-lymphocytosis in such patients. Plasma samples were obtained serially from 14 adult patients and 14 adult controls. One-half ml of plasma was injected intraperitoneally into C3H/He male mice, and absolute numbers of surface immunoglobulin-bearing cells in the mouse peripheral blood were counted. Fractionation of plasma samples was performed. The biologically active plasma fraction, tentatively termed B-lymphocytosis factor (BLF), was cultured with normal human peripheral blood lymphocytes to determine in vitro lymphocyte transformation. The solubility of 125I-labelled BLF in various organic solvents was examined. Plasma obtained from burned patients induced a marked increase of IgG, IgA, and IgM-bearing cells in the peripheral blood of mice within 3 hours after injection. The B-lymphocytosis activity of normal plasma was not significant. The molecular weight of BLF was estimated to be close to that of ribonuclease A (M.W. 13,400). Transformation of normal human peripheral blood lymphocytes was observed when cultured with BLF. 125I-labelled BLF was not soluble in organic solvents. These data suggest that BLF is not a lipopolysaccharide, and plays a role in regulation of B-lymphocyte levels in burned patients.
In radioimmunoassay of digoxin by the dextran-coated charcoal method, duration of the incubation period for the antigen-antibody reaction and time allowed for dextran-coated charcoal to absorb the free antigen are critical factors. We compared effects of prolonged periods of absorption on results obtained with five commercial kits. In all cases, the digoxin concentrations increased linearly with absorption time, but the increase varied among kits. Kallestad and New England Nuclear 3h-kits and the Squibb 125I-kit showed less increase (i.e., gave relatively consistent results after various periods of absorption); the Schwarz/Mann 125i-kit and Clinical Assays 3h-kit showed greater increases. Precautionary steps are outlined for radioimmunoassays by this technique.
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