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Biomedical subjects

H L Coetzee

Publications and source records attributed to H L Coetzee.

4 recordsLinked to original sources
PubMed2000

The optical microscope--en route to extinction?

Change in medical curricula is a worldwide trend. Concomitant with this is a change in the role of the microscope and its contribution to a student's career. In this questionnaire-based study the perceptions of medical, dental and veterinary students to microscopy and alternative forms of histology tuition, as well as the role of microscopy in their future careers, was assessed. Veterinary students indicate a strong preference for the microscope and microscopy training due to the importance of the microscope in their chosen career, whilst medical and dental students are somewhat ambivalent. However, the students would not choose to eliminate the microscope entirely from their studies.

Attitude of Health Personnel↗
PubMed1995

Characterization of mucus glycoproteins in the intestinal mucosa of the African elephant (Loxodonta africana) following lectin histochemistry.

The glycoproteins of the small intestines, caecum and colon of three adult elephants and one recently weaned elephant calf were examined by means of lectin histochemistry. Tissue sections were histochemically stained with peroxidase-labelled concanavalin A (Con A), asparagus-pea (TPA), peanut (PNA) and wheat-germ (WGA) lectins. Con A and TPA showed no binding activity in the intestinal tract of the adult elephants or the duodenum and ileum of the elephant calf, but did show a small amount of binding activity in the caecum and colon of the calf. WGA bound very intensely throughout the intestinal tracts of the adults and of the calf--especially with the goblet cells located in the crypts of Lieberkuhn and the glands of Brunner--decreasing in intensity towards the luminal surface of the intestinal tract. PNA stained the glands of Brunner of the duodenum faintly and the goblet cells of the ileum moderately, with no staining of the caecum and faint staining of the colon. These results show the distribution of Con A-, WGA-, PNA- and TPA-binding sites, and the changes that take place in the type of glycoprotein secreted after a change in the diet of the animal.

Animals↗
PubMed1994

A histocytochemical study of mucus glycoproteins or mucins in the intestinal tract of the African elephant (Loxodonta africana).

The distribution of neutral mucins, sialomucins and sulphomucins was determined histochemically in the duodenum, jejunum, ileum and colon of the African elephant (Loxodonta africana). The techniques used were periodic acid-Schiff (PAS), alcian blue/periodic acid-Schiff (AB-PAS), high-iron-diamine/alcian blue (HID-AB), alcian blue at varying pH solutions and alcian blue at high temperature after methylation and saponification. Acid mucins appear to dominate neutral mucins, the latter decreasing toward the large intestine. Sulphomucins and sialomucins occurred in almost equal amounts throughout the intestinal tract, with a slight decrease of sialomucins toward the colon.

Animals↗