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H L Kummerfeld

Publications and source records attributed to H L Kummerfeld.

6 recordsLinked to original sources
PubMed1981

Influence of bovine cervical mucus samples and storage conditions on sperm migration in vitro.

In vitro sperm migration assays were performed using bovine spermatozoa and cervical mucus. Experiments were designed to test the effects of storage temperature, method of storage, duration of storage, and source of cervical mucus. Significant variation in migration of spermatozoa was due both to differences in mucus samples and to short-term mucus storage at temperatures ranging from ambient to -196 degrees C. The parallel-orienting effect of cervical mucus on migrating sperm was shown to be a major factor in quantitative assays based upon migration distance. Thus, comparisons of migration among different specimens of semen likely will be biased unless the tests are run simultaneously. Implications of these results are discussed relative to the performance of quantitative sperm migration assays in the clinical or research laboratory.

Animals↗
PubMed1981

Polyacrylamide as a substitute for cervical mucus in sperm migration tests.

A synthetic migration medium for capillary sperm migration studies was developed. Parallel sperm migration in 1.8% polyacrylamide cross-linked with 0.042% N,N'-methylene bis acrylamide was similar to sperm migration in bovine cervical mucus. Bull spermatozoa varying widely in migration distances in bovine cervical mucus maintained similar relative migration distances in this synthetic medium. The advantages of the synthetic medium are its availability in large quantities, its uniformity, and its stability. There was no change in parallel sperm migration distances in the synthetic medium stored at 4 degrees C for up to 4 months. Use of this synthetic medium for human or bovine sperm migration studies would appear to overcome problems associated with the variability of cervical mucus.

Acrylamides↗
PubMed1978

Embryonic mortality in dairy cows estimated by nonreturns to service, estrus, and cyclic milk progesterone patterns.

Cyclic patterns of progesterone in milk were studied in 262 Holstein cows. Milk progesterone data were obtained from 153 cows for at least 75 days after conception as indicated by continuous high progesterone concentrations in milk for 28 days or more following artificial insemination. Cycling was reinitiated in 11 of these cows between 28 and 75 days after breeding, for an estimated 7.2% rate of embryo-fetal mortality. This was considerably less than a corresponding rate of 22.7% estimated for this herd by the delayed returns to estrus (28 to 75 days). In 350,180 cows inseminated with semen from Holstein bulls the estimated embryo-fetal mortality by the same delayed return to service method was 12.5%. Biases in this latter method of estimation are discussed.

Animals↗
PubMed1976

Spermagglutinin titers and their relationship to fertility in isoimmunized female rabbits.

The sera from 48 female rabbits immunized by a series of multiple intradermal injections of washed epididymal, washed ejaculated, and beta-amylase-treated rabbit spermatozoa in complete adjuvant were examined for spermagglutinins by the Kibrick gel agglutination test and a slight modification of the Shulman capillary agglutination test. Control animals receiving the adjuvant or saline usually had no positive titers. All three antigenic preparations produced similar titers, positive at dilutions as high as 8192-fold with Kibrick test and 256-fold with the Shulman test. Maximal titer development was reached 4 to 6 weeks after starting the immunization, and positive sera were obtained from some does for 25 weeks. The correlation coefficients between positive titers obtained by the two tests were r = 0.91 during the first 10 weeks and 0.41 at 15 to 25 weeks after immunization.

Animals↗