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Biomedical subjects

H L Mueller

Publications and source records attributed to H L Mueller.

11 recordsLinked to original sources
PubMed1990

Particle distribution in lung and lymph node tissues of rats and dogs and the migration of particle-containing alveolar cells in vitro.

Whole rat lungs and individual dog lung lobes were instilled either with low numbers (10(7)) or high numbers (10(9)) of fluorescent polystyrene microspheres (PLM), or with saline alone. Particle distributions in dog and rat lung lobes and tracheobronchial lymph nodes (TBLN) were studied up to several weeks after particle instillations using methacrylate-embedded tissues and epifluorescence light microscopy. Free alveolar cells were obtained from rats and dogs by lung lavage 1 or 7 d after particle instillations. Lavaged cells were tested for directed migration toward the chemoattractant N-formylmethionyl-leucyl-phenylalanine (FMLP). Random migration in the absence of the FMLP was used as a control. The dog lung interstitium contained many more particles than did the rat lung interstitium, and particle numbers in interstitial and TBLN cells of dogs were higher than in those of rats. FMLP enhanced the number of migrating cells about twofold. Increasing particle numbers in lavaged phagocytes (greater than 10 particles/phagocyte in dogs; greater than 20 particles/phagocyte in rats) decreased their ability to migrate. The higher fractions of particles in the dog lung interstitium are thought to be an important reason for prolonged retention and increased TBLN transport of deposited particles in dogs as compared with rats. Our results suggest that cell mobility is lost after ingestion of high numbers of particles, and that this occurs earlier with dog than with rat lung cells.

Animals↗
PubMed1989

Uptake of inert particles by dog alveolar macrophages in vitro--a comparison of monolayer and suspension techniques.

This study was conducted to determine if significant differences in phagocytosis occurred in pulmonary alveolar macrophages in suspension or monolayer cultures. Dog alveolar macrophages were incubated with different numbers of fluorescent polystyrene latex microspheres, both in suspension and in monolayer cultures. The particle uptake by cells was linear for 10-100 min and the fraction of cells that contained particles was similar for both incubation techniques. Differences between carboxylated and non-carboxylated microspheres were statistically not significant. The overall particle numbers associated with cells were 2-5 times higher for cells incubated in suspension compared to adherent cells. Many more cells were found with low particle numbers at all incubation times using the monolayer technique compared to the suspension technique. When cells were treated with xylene for 1 h after different times of incubation in vitro, a higher decrease in particle numbers in cells was found in suspension cultures than in monolayer cultures. This finding suggests higher particle binding when cells were incubated in suspension, but particle numbers after xylene treatment were still higher in cells incubated in suspension than in monolayers. A comparison with the uptake of similar particles by dog alveolar macrophages in vivo suggests that the suspension cultures were more similar to the events in the dog lung, but uptake rates were about a factor of two lower with suspended cells than those seen in vivo.

Animals↗
PubMed1977

Maintenance of protection in patients treated for stinging insect hypersensitivity: a booster injection program.

A program of annual booster injections of mixed stinging insect whole body extract was given to 133 patients who had previously had three years or more of immunological treatment for systemic reactions of Hymenoptera stings. Two patients who were restung had mild symptoms that were evidenced by a few scattering hives with itching. No patient on a dose of 0.2 ml of 1:10 dilution extract had any reaction when they were restung. It is believed, therefore, that this program merits continuation with that dosage for a period long enough to determine its true effectiveness. There is some indication that this is an improvement over the program which stops treatment after three years. There is further support for the belief that this condition and the treatment are dose related. There is also evidence for my belief that this sensitivity, and the treatment, differ from the usual atopic sensitivity in antibody-antigen response.

Evaluation Studies as Topic↗
PubMed1975

Prolonged interval hyposensitization therapy in hymenoptera-sensitive patients.

The Committe on Insects of the American Academy of Allergy has conducted a 10-year study of 397 patients with Hymenoptera allergy treated with glycerosaline or aqueous whole body mixed Hymenoptera extracts at intervals of 2 or 3 months for the maintenance doses. Patients had few reactions, although in some cases local reactions presented lengthening of the interval. The clinical response was similar to that from conventional therapy using the 4-wk interval for maintenance doses. This type of therapy offers the added advantages of fewer patient visits and decreased cost.

Adolescent↗