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Biomedical subjects

H Lampe

Publications and source records attributed to H Lampe.

29 records · Page 2Linked to original sources
PubMed1990

Whole cell preparation of squamous cell carcinoma for cytometric analysis of DNA.

To date, analysis of the DNA content of head and neck squamous cell carcinomas has relied on the homogenation of the entire tissue specimen and subsequent staining and quantitation of the naked nuclei. This methodology does not make allowance for the extremely variable nature of these tumors with respect to their cellular composition. Further, by destroying the cytoplasm and cell membranes, this methodology makes it impossible to distinguish the DNA content of the tumor cells from that of the background stromal and inflammatory cells. The authors present a methodology for the selective exclusion of inflammatory cell infiltrates from the DNA analysis of these tumors. Using this technique, it has been found that exclusion of the inflammatory cells allows the investigator to look more specifically at the malignant cell population. This has been most helpful in those samples in which the tumor cells have been diploid or near-diploid. With this technical refinement, the relationship between DNA ploidy and clinical prognosis may be more accurately assessed.

Aged↗
PubMed1984

Epithelial-myoepithelial carcinoma of the salivary gland.

Epithelial-myoepithelial carcinoma of salivary gland is a rare tumor only recently described in the English language literature. This paper adds four new cases to the 30 which have previously been reported. The clinical presentation of this unusual neoplasm is documented in the accompanying case reports and the appropriate surgical management outlined. Follow-up ranges from six months to 12 years. The histopathological characteristics of this interesting tumor, in particular its classical biphasic cellular appearance, are described in detail both by light and electron microscopy. On the basis of histologic appearance and biological behavior, the authors postulate that epithelial-myoepithelial carcinoma is an uncommon variant of adenoid cystic carcinoma.

Aged↗
PubMed1980

Use of computer assisted tomography in the diagnosis of lesions in and around the sphenoid sinus.

The use of the CT scanner and its contribution to the diagnosis of lesions in and around the sphenoid sinus has been reviewed. The usefulness of this new imaging technique is discussed. Five representative cases demonstrating special diagnostic tests in conjunction with the CT scanner are discussed. The CT scanner combines low cost with minimal risk to the patient. It is a valuable tool in the investigation of the sphenoid sinus.

Adult↗
PubMed1978

On the problem of optimum digitlaization in man.

The low therapeutic index of cardiac glycoside requires an optimum individual digitalization. This is also a difficulty in the case of known serum glycoside concentrations because individual differences of biochemical and physiological factors (which influence both therapeutic and side effects) are important. As a rule the physician's criterion for an optimum digitalization is that side effects are avoided. This investigation attempted to find a measure for the effect of glycosides by noninvasive recording of parameters of myocardial contractility (systolic time intervals, STI). The measurements were made under oral digitalization with digoxin (Dilanacin). The serum glycoside concentration was determined by the radioimmunoassay method. The changes in STI were investigated in the rage of 1 to 3 ng digoxin per ml serum. In this concentration a significant correlation was demonstrated between STI shortening and glycoside level. It was found that the shortening of STI was maximum at the lowest concentration (1 ng/ml). It appears that the control of STI is for the physician a useful aid for an optimum digitalization in man.

Adult↗
PubMedDate not supplied

Dermoid cyst of the floor of the mouth diagnosed by fine needle aspiration cytology: a case report.

BACKGROUND: Dermoid cyst of the floor of the mouth is a rare, benign lesion requiring surgical intervention. To our knowledge, the preoperative diagnosis of this entity by fine needle aspiration (FNA) cytology has not been previously reported in the cytologic literature. CASE: The patient presented with a massive swelling of the floor of the mouth that displaced the tongue superiorly. A computed tomographic (CT) scan demonstrated a cystic lesion thought to be consistent with a lymphangioma. FNA was performed without complications and demonstrated numerous anucleated and some nucleated squames consistent with a diagnosis of dermoid cyst. Establishment of the correct diagnosis by FNA enabled the surgeon to proceed with the appropriate operation; that outcome might not have been possible had the erroneous CT diagnosis of lymphangioma been trusted. CONCLUSION: A preoperative diagnosis of dermoid cyst of the floor of the mouth was established by FNA. In contrast to reports in the older literature, the FNA procedure caused no complications. In patients presenting with lesions of the floor of the mouth, FNA should be the diagnostic procedure of first choice.

Adult↗
PubMedDate not supplied

Comparative study of the acute nephrotoxicity from standard dose cisplatin +/- ifosfamide and high-dose chemotherapy with carboplatin and ifosfamide.

The nephrotoxic effects of different platinum compounds based combination chemotherapies were compared. Chemotherapy consisted of either cisplatin fractionated over 5 days (5 x 20 mg/m2) or given as a single-day infusion (1 x 50 mg/m2) plus ifosfamide (4 g/m2) or high-dose chemotherapy was applied including carboplatin (3 x 500 mg/m2) and ifosfamide (3 x 4 g/m2) fractionated over three consecutive days. Conventional parameters such as serum creatinine and glomerular filtration rate (GFR), as well as urinary protein excretion of N-acetyl-beta-D-glucosaminidase (NAG)) and alpha 1-micro-globulin were assessed in 52 patients. Fractionation over 5 days without adding other nephrotoxic agents, i.e. ifosfamide, prevented decreases in GFR following cisplatin, whereas the combination of conventional dose cisplatin and ifosfamide, given as a single-day infusion, and high-dose carboplatin/ifosfamide yielded a pronounced fall of GFR. All groups showed increases in the urinary excretion levels of serum derived proteins and NAG, but with significant differences; about 2 to 3-fold for 5-days cisplatin, 3 to 5-fold for single-day cisplatin/ifosfamide, and 20 to 35-fold for high-dose chemotherapy. Thus, conventional approaches can reduce but not prevent the nephrotoxicity of cisplatin-based chemotherapy. In particular, high-dose chemotherapy regimens including carboplatin and ifosfamide are associated with comparable or even higher nephrotoxicity to single-day cisplatin/ifosfamide. In the light of the long-term consequences of persistent renal damage prevention of nephrotoxicity should be further improved.

Acetylglucosaminidase↗
PubMedDate not supplied

Intraoral and transoral fine needle aspiration. A review of 25 cases.

During a four year period, 25 intraoral and transoral fine needle aspirations were performed. The sites aspirated were the parapharyngeal space (7), palate (4), floor of mouth (3), tongue (3) and a variety of other intraoral sites (8). Squamous cell carcinoma, the most common malignancy encountered, was correctly diagnosed in six cases. Other malignancies correctly diagnosed were 2 malignant lymphomas, 1 mucoepidermoid carcinoma and 1 adenoid cystic carcinoma. One malignant case called "carcinoma, probably adenoid cystic carcinoma" proved to be a mucoepidermoid carcinoma. One case called "suspicious for squamous carcinoma" was found to be a branchial cleft cyst. In another case a diagnosis of carcinoma in situ of the tongue was suggested and confirmed by subsequent biopsy, but the underlying adenoid cystic carcinoma was missed. Of seven benign entities, five were correctly diagnosed by fine needle aspiration (FNA), and in three cases the FNA diagnosis was equivocal. Unsatisfactory aspirates were obtained in four cases. Despite some obvious problems, we consider FNA to be especially useful for the evaluation of parapharyngeal masses, which are usually not accessible to routine surgical biopsy, and for palatal masses, which are usually submucosal and therefore often difficult to biopsy.

Biopsy, Needle↗
PubMedDate not supplied

Fine needle aspiration of medullary carcinoma of the thyroid. Cytomorphology, immunocytochemistry and electron microscopy.

OBJECTIVE: To analyze of medullary carcinoma of the thyroid (MCT) diagnosed by fine needle aspiration (FNA) utilizing cytomorphologic features and ancillary studies. STUDY DESIGN: Nine cases of MCT were collected, and the cytomorphologic findings were reviewed. Additionally, immunocytochemistry, immunoelectron microscopy and ultrastructural examination results were reviewed for selected cases. RESULTS: In five cases, loose groups predominated over single cells, whereas single cells predominated in three cases. One case showed only highly cohesive groups of cells. Most cells were round to oval, and every case had some degree of plasmacytoid morphology. Spindle-shaped cells were predominant in one case and were occasionally noted as a subpopulation in the other cases. Binucleation was noted in seven cases, and scattered, abnormally large nuclei were identified in five cases. The cytoplasm was moderate to abundant and delicate in all cases. Routine immunocytochemical staining for calcitonin and chromogranin was positive in three of four cases, and staining positive for the markers was detected by immunoelectron microscopy in two cases. In four cases, electron microscopy revealed neurosecretory granules. CONCLUSION: The cytomorphologic appearance of medullary thyroid carcinoma is highly distinctive, and the diagnosis can be corroborated by appropriate ancillary studies.

Adult↗