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H Lipton

Publications and source records attributed to H Lipton.

10 recordsLinked to original sources
PubMed1990

Detection of restricted predominant epitopes of Theiler's murine encephalomyelitis virus capsid proteins expressed in the lambda gt11 system: differential patterns of antibody reactivity among different mouse strains.

Intracerebral injection of mice with Theiler's murine encephalomyelitis virus results in chronic demyelination in susceptible strains, and serves as a model system for the study of multiple sclerosis. The role of individual epitopes in the disease process remains to be elucidated. Random fragments of DNA from the viral capsid protein genome covering the coding regions from VP1, VP2, and VP3 have been expressed in the lambda gt11 vector system. Fusion proteins from the clones were expressed and probed with antibodies from both resistant and susceptible strains of mice. Each strain displays a distinctive pattern with certain fusion proteins recognized by all of the strains and others recognized uniquely by either the susceptible or the resistant strains.

Animals↗
PubMed1989

The effect of cyclosporine on the use of hospital resources for kidney transplantation.

Over the past decade the clinical results of kidney transplantation have improved substantially, with much of the benefit being attributed to the introduction in late 1983 of the immunosuppressive drug cyclosporine. To assess the effect of cyclosporine on the use of hospital services, we studied 702 patients who received kidney transplants at the University of California, San Francisco, between July 1982 and June 1986. All services were priced in constant 1985 dollars, and multiple regression analysis was used to adjust for changing patient and hospital characteristics. The introduction of cyclosporine for patients receiving kidneys from cadavers was associated with a significantly shorter adjusted mean postoperative stay (26.4 days as compared with 37.0 for patients not taking the drug; P less than 0.0001) and lower adjusted mean hospital charges ($28,649 as compared with $37,895; P less than 0.0001), although cyclosporine was not associated with changes in the use of services by patients who received transplants from living related donors. Cyclosporine was also associated with a reduction in the use of certain ancillary services, such as laboratory tests and radiographic procedures. In patients without diabetes who received cadaver kidneys, a sequential cyclosporine regimen (in which a combination of antilymphoblast globulin, prednisone, and azathioprine was given before cyclosporine) reduced the use of hospital services even more than did a cyclosporine regimen in which the combination was not given. The results suggest that new medications, such as cyclosporine, that reduce the frequency of complications and improve outcomes may also reduce the use of hospital resources.

Adult↗
PubMed1987

Lansing poliovirus infection in mice: antibody demonstrable by enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation but not by neutralization.

Adult mice infected intracerebrally (i.c) with the Lansing strain of type 2 human poliovirus (HPV2) failed to develop a systemic neutralizing antibody response until 2 months post-infection (p.i). In contrast, an enzyme-linked immunosorbent assay (ELISA) demonstrated an antibody response of IgM and IgG classes beginning at day 1 p.i. with peak levels reached by 5 weeks p.i. This response was slightly greater in paralyzed than in nonparalyzed animals. Immunoprecipitation of poliovirus proteins from cytoplasmic extracts and disrupted purified virion preparations revealed antibodies to three capsid proteins, two capsid precursor proteins, and one nonstructural protein. Finally, neither neutralizing antibody nor definite virus replication was detected after oral, intraperitoneal, or intravenous routes of inoculation. We conclude that the lack of a systemic neutralizing antibody response in mice is probably due to an insufficient amount of infectious virus and consequently viral neutralizing epitopes reaching extraneural lymphoid tissues.

Animals↗