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Biomedical subjects

H M Bianchini

Publications and source records attributed to H M Bianchini.

8 recordsLinked to original sources

Update on Pan-American research on anaerobes: the situation in Argentina.

The study of anaerobic bacteria became important in Argentina when the first outbreak of botulism was reported in Mendoza in 1922. In the 1970s, the emphasis was on the development of methods to obtain suitable samples for isolation and identification of anaerobic bacteria. In 1985, a working group belonging to the Argentine Association of Microbiology was organized to study anaerobic bacteria and infections. Last year, I became involved with the immunologic, microbiological, and epidemiological features of periodontitis. Other investigators in our country are studying Clostridium difficile, Campylobacter species, and Helicobacter species as well as lactobacilli, Propionibacterium species (as probiotics to protect the gastrointestinal tracts of children), anaerobic veterinary infections, food contamination by anaerobes, and biotechnological applications of anaerobes.

Argentina↗

Synergy characterization for Enterococcus faecalis strains displaying moderately high-level gentamicin and streptomycin resistance.

Synergy of 14 Enterococcus faecalis strains displaying moderately high-level aminoglycoside resistance (MICs, 500 and 256 to 1,000 micrograms/ml for gentamicin and streptomycin, respectively) was characterized by time-kill studies. All strains proved resistant to penicillin plus the respective aminoglycoside. Strains with moderately high-level aminoglycoside resistance should be considered to exhibit high-level resistance in severe infections.

Drug Resistance, Microbial↗

Abscess caused by vancomycin-resistant Lactobacillus confusus.

Several isolates of vancomycin-resistant Lactobacillus confusus from human sources have been described, but to our knowledge, no well-documented infection attributable to this organism has been published. A thumb abscess caused by this bacterium in a healthy 49-year-old male is reported here. He was successfully treated by surgical drainage and cephalothin.

Abscess↗

Mycobacterium gastri arthritis: septic arthritis due to Mycobacterium gastri in a patient with a renal transplant.

We describe an 18-year-old man with a renal transplant who developed septic metacarpophalangeal arthritis due to Mycobacterium gastri. He had several episodes of crystal induced synovitis, and treatment with intraarticular steroids was complicated 3 months later by iatrogenic septic arthritis. Appropriate treatment based on in vitro drug susceptibility was successful. This seems to be the first case of articular infection and the third report of human infection caused by this atypical mycobacteria.

Adolescent↗

[Resistance of Staphylococcus aureus to methicillin].

Data on methicillin-resistant Staphylococcus aureus have not been presented before in Argentina. Due to non adequate microbiologic techniques, this phenomenon may be overlooked. Heterogeneous strains are only detected with 5% NaCl agar, low temperature (30 degrees C) or prolonged incubation and/or high inocula (10(6) C.F.U.). Minimal inhibitory concentrations (MICs) of methicillin were studied by the agar dilution technique in antibiotic medium number 4 with and without 5% salt. This study was performed for 107 strains at 37 degrees C, after 24 and 48 h incubation, with an inoculum size of 10(6) CFU. S. aureus strains were considered resistant whenever MICs were greater than or equal to 8 mg/l. Forty seven (44%) methicillin-resistant strains were detected. Thirty one were high level resistant mutants and sixteen showed the heterogeneous phenomenon. Due to the high frequency of resistant strains among clinical specimens in this country, the routine use of elaborate screening tests with special media and or prolonged incubation time is justified.

Argentina↗

[Identification of Streptococcus viridans in clinical specimens].

In a 4 year prospective study of "viridans" or non-groupable streptococci 100 clinically significant strains were isolated. Strains were defined as significant when found in two or more samples of blood cultures, or when seen on Gram staining together with inflammatory reaction. Fifty-two strains were speciated by 10 physiological characteristics. In 42 cases additional tests were performed. Only 16 isolates were unidentifiable by the techniques used. Sources of isolates included purulent lesions (53%) (mainly S. milleri and S. mitior); endocarditis (27%) (S. bovis I, S. sanguis, S. mutans, S. bovis II) and urinary tract infections (10%) (S. bovis II, S. milleri).

Endocarditis, Bacterial↗