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Biomedical subjects

H M Jiang

Publications and source records attributed to H M Jiang.

10 recordsLinked to original sources
PubMed2001

[Listeria monocytogenes induces thymocyte apoptosis in mice].

The murine thymocyte apoptosis induced by Listeria monocytogenes(LM) was detected with morphology, FCM, and DNA electrophoresis. The results were that LM elicited typical morphological changes of thymocyte apoptosis; the typical apoptosis peak was displayed with FCM, and typical "ladder pattern" with agarose gel electrophoresis. The apoptotic cells were found at 8 h after the mice had infected LM and reached climax at 48 h. The thymus weight significantly reduced at 16 h, and reached the lowest at 48 h after the mice had infected LM. The percentage of apoptotic cells was raised with the increasing of LM. These results suggest that LM induces thymocyte apoptosis in dose- and time-dependent manner.

Animals↗
PubMed2001

[Experimental study on isolated testes with ischemia/reperfusion injury].

OBJECTIVE: To investigate the injury on isolated testes induced by ischemia/reperfusion(I/R), and the protective effect of Yisheng injection on the injury. METHODS: Twenty-six isolated cadaver testes contributed by 13 persons were preserved with 4 degrees C 250 ml hypertonic citrate alloxuric (HCA) solution and then reperfused with 37 degrees C 500 ml HCA. Solution of experimental group contained 500 micrograms/ml Yisheng injection. In simple cold preservation test, involving in 8 experimental and 8 control testes, a series of time points (6, 12, 18, 24, 36, 48, 60, 72 hours) were set to harvest. 10 testes (1 testis respectively on 6, 12, 18, 24 and 36 hours in experimental and control groups) were reperfused with 37 degrees C HCA for 6 and 12 hours. Histological and histochemical changes were observed. RESULTS: In the experimental testes, 4 degrees C cold preservation in 24 hours could not induce obvious pathologic changes. After 24 hours, changes such as swelling, vacuolar degeneration or detachment of endothelial cells (ECs), separation between basement membrane and seminiferous epithelium, mal-alignment of spermatogenous cell and edema of mesenchyme could be observed. In the testes preserved for 12 hours, the activity of lactic dehydrogenase(LDH) and succinic dehydrogenase (SDH) increased, then fallen after 24 hours. The activity of Nitric oxide synthetase(NOS) decreased after 18 hours. All changes were more obvious after following 37 degrees C reperfusion. In the control testes, swelling and vacuolar degeneration of ECs occurred on 12 hours cold preservation, and injury was worse along with the prolongation of cold preservation time. Pathologic changes of ECs, seminiferous epithelium and mesenchyme were serious after 37 degrees C reperfusion. CONCLUSION: 4 degrees C cold preservation in 24 hours can only cause mild ECs' injury, and obvious abnormal testes' histological profile can be observed beyond 24 hours. 37 degrees C reperfusion will make injury worse. Yisheng injection can keep isolated testes histologic structure well in 24 hours cold preservation, and it has protective effect on I/R injury.

Cryopreservation↗
PubMed2000

[Intravenous infection of C. albicans induces apoptosis of cortical thymocytes in mice].

In this study, we aimed to understand the location of apoptotic cells in murine thymus and phenotype of murine thymocytes involved in C. albicans induced apoptosis. Results showed that significant increase of apoptotic cells in thymus began at 6 h after intravenous injection of C. albicans and kept increased at 12 h, 18 h and 24 h. Quantitative changes of thymocytes with different phenotype measured by flow cytometry revealed that percentage of CD3+ and CD8+ cells were significantly reduced compared to the controls. Moreover in situ cell death detection of thymic tissue revealed that apoptotic cells induced by C. albicans mainly located in the thymic cortex, while in the thymic medulla a very small number of thymocytes was involved. Taken together our study indicates that C. albicans induces apoptosis of murine thymocytes, moreover the apoptosis possibly involves the major immature CD3+ CD4+ CD8+ cells in the thymic cortex and minor mature CD3+ CD4- CD8+ cells in the thymic medulla.

Animals↗
PubMed1997

[Clinical application of inspiring psychological nursing in vaginal delivery].

A new nursing method named Inspiring Psychological Nursing was initiated and applied to 176 primigravida undergoing vaginal delivery from Jan. to Jun. 1994. 202 primigravida who received general medical care during vaginal delivery from Jan. to Jun. 1993 was served as control group. No significant differences were found between the two groups regarding to age, gestational weeks, times of pregnancy, fetal position and newborn's weight. However significant differences were found regarding the duration of the second stage of labor and incidence of newborn asphyxia. Primigravida in the control group usually delivered after 60 min. and carried a higher incidence of newborn asphyxia, being 6.7%. It's concluded that Inspiring Psychological Nursing could significantly improve pregnancy outcome.

Asphyxia Neonatorum↗
PubMed1991

[Significance of measurement of serum and cerebrospinal fluid trace elements in the diagnosis of brain tumors].

Cu, Zn, Cd, Mn, Se in serum and Cu, Cd and Mn in cerebrospinal fluid (CSF) of 150 patients were measured by flame, flameless and hydride generation atomic absorption spectrophotometry. The patients were divided into three groups: malignant brain tumor group (MTG), benign brain tumor group (BTG), and non-neoplastic neurological disease group (no evidence of brain tumor group, NENG). The results were as follows: 1. Serum Cu, Zn, Cd, Mn, Se levels before operation were higher than those after operation; 2. Cu Cd, Mn serum levels were positively correlated with the CSF in the three groups. The correlation coefficients of serum Cu-CSFCU were 0.8935 (MTG), 0.6074 (BTG), 0.5349 NETG); serum Cd-CSFCd 0.7963, 0.2671, 0.5398; serum Mn- CSF Mn 0.5855, 0.4474, 0.5163, (P less than 0.01). 3. Serum and CSF Cu levels in MTG were significantly higher than in BTG and NETG (P less than 0.01). 4. A group of discrimination function equations was established with stepwise discrimination analysis. The six factors, i. e. serum Cu, Cd, Mn, Se and CSF Cu, Mn were significant in the equations. The conformation rate of diagnosis and discrimination by the equations in the malignant and benign brain tumors with clinical diagnosis was 81 percent. The equations could describe the relationship of coordination and antagonism among trace elements in serum and CSF. The authors believe that discrimination equations may have potential use both in diagnosing and discriminating patients in the 3 groups mentioned above and in following patients after excision of the brain tumor.

Adolescent↗
PubMed1991

[The clinical significance of multifactor discrimination and analysis of maternal serum Cu, Zn, Cd, Mn contents in the diagnosis of abnormal fetus].

In this paper we present pair-comparison test with serum contents of Cu, Zn, Cd, Mn, Se in pregnant women with neural tube defects fetus (NTD), pregnant women with normal fetus and healthy with no pregnancy using Bayes Stepwise Discrimination Analysis and Fisher Discrimination Analysis. The results showed: NTD of fetus was related to deficiency of maternal serum in Cu, Zn, Mn and to excess of Cd. The maternal serum levels of Cu, Cd and Mn can be used as sensitive indicator to detect intrauterine abnormality at an early stage. The low levels of maternal serum in Zn and Se were not a specific indicator of NTD but a characteristic shared by all pregnant women. It is important to pay attention to supplying pregnant women at an early stage with Zn, Se to meet the needs of normal growth of the fetus and prevent foetal abnormality. With stepwise discrimination we established two equations by Cu, Zn, Cd, Mn used for distinguishing normal from abnormal fetus. The accordant rate of clinical discrimination with classification output for inside examples was 93%. In the equation established by Fisher principle Mn had the greatest rate, namely, 37.6%.

Adult↗
PubMed1990

Clinical significance of hair cadmium content in the diagnosis of mental retardation of children.

Cadmium (Cd) is known to be closely related to the development of children's intelligence. To investigate the clinical significance of the hair cadmium content in the diagnosis of mental retardation (MR), 415 normal and 85 MR children's hair cadmium contents were measured by 79-1 anodic stripping voltmeter. The data were processed by an Apple-II microcomputer. Comparing the median value and using the Chi-square test the Cd contents in the hair of both male and female MR children were obviously higher than those of normal children (P less than 0.001). If the Cd content in the hair of male children is higher than 0.239 microgram/g, and higher than 0.180 microgram/g in the female, MR can be diagnosed. The sensitivity and specificity of the test are greater than 90%; false positive and false negative rates are lower than 10%. Hair Cd can be used as an additional criterion in the diagnosis of MR and may be important in the screening for MR.

Cadmium↗