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H M Mathews

Publications and source records attributed to H M Mathews.

45 records · Page 3Linked to original sources
PubMed1975

Field application to malaria studies of the passive haemagglutination (PHA) test with lyophilized cells.

Lyophilized cells for the passive (formerly indirect) haemagglutination test for malaria were tested in a small field trial. A comparison was made with results obtained with samples from the same persons by another method in another laboratory (Center for Disease Control, Atlanta, GA, USA). The use of lyophilized cells of standardized reactivity clearly adds to the simplicity and rapidity of the test, and justifies its recommendation for use in field studies.

Freeze Drying↗
PubMed1973

Interpretation of IHA titres for the study of malaria epidemiology.

The results of IHA test surveys of persons with malaria parasitaemia in Ethiopia and the Philippines suggest that the antibody response may be influenced by the frequency and intensity of the antigenic stimulations and also be age-dependent. Antibody frequency distribution curves from four different areas suggest that the shape of such curves can provide some information about the endemicity of malaria. Results of similar and of parasitological surveys in Bangladesh, Ethiopia, Haiti, and the Philippines were compared and related to available malaria surveillance information. The results indicate that a serologic population profile may provide an indication of the history and status of malaria. Technical aspects of the IHA test are reviewed; it may be desirable to use homologous antigens instead of a simian Plasmodium antigen. To obtain the most useful additional epidemiological information about a malaria situation, serologic data need to be age-related, and longitudinal surveys are usually more informative than a cross-sectional survey.

Adolescent↗
PubMed1969

Seroepidemiological studies by indirect haemagglutination tests for malaria. Military recruit collections from Argentina, Brazil, Colombia, and the United States of America.

Seroepidemiological studies of 10 956 sera of military recruits from 4 countries in the Western Hemisphere suggest that the indirect haemagglutination test employing an antigen prepared from Plasmodium knowlesi adsorbed to human group O erythrocytes may be useful in epidemiological studies of malaria. Based on a titre of 1: 8, or higher, in a micro-titration of serum, the prevalence of serological reactors in the collection of sera from USA recruits was 0.9%, in the Argentina collection 4.6%, in the Brazil collection 20.8%, and in the Colombia collection 21.3%. In Brazil and Colombia the distribution of serological reactors correlated with the distribution of malaria as determined by active and passive surveillance. In Argentina, serological reactors were found in states where active and passive surveillance indicated that malaria had been eradicated years ago.

Brazil↗
PubMedDate not supplied

Isoenzyme comparison of axenic Giardia lamblia strains.

We obtained isoenzyme patterns by polyacrylamide gradient gel electrophoresis (PGGE) of water-soluble protein fractions prepared from trophozoites of 11 axenic G. lamblia strains. The strains were isolated from animals and humans (both symptomatic and asymptomatic) from various geographic locations. Isoenzymes were also separated by isoelectric focusing. Of 12 enzymes attempted, eight exhibited well-defined and reproducible isoenzyme patterns by PGGE, based on which the strains were grouped into four zymodemes. Although the 11 strains were grouped into four zymodemes based on PGGE, no correlation between zymodeme and the known characteristics of the strains existed. Thus, a high degree of characteristic sharing appears to occur among genetically different G. lamblia strains.

Animals↗