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Biomedical subjects

H M Miller

Publications and source records attributed to H M Miller.

13 recordsLinked to original sources
PubMed1992

Women's views of postpartum sibling visitation.

The purpose of this study was to identify women's views of postpartum sibling visitation. Fifty-eight Lamaze-prepared, married multigravidae expressed their views on sibling visitation in a semistructured interview conducted during their postpartum hospitalization. The majority of women (84%) chose to have their children visit. Women identified positive and negative aspects of sibling visitation, explained reasons for not having siblings visit, and discussed reactions to separation from their children. The women believed both siblings and mothers benefitted emotionally from the visit. Negative aspects of sibling visitation included emotional reactions of mothers and siblings, lack of control between siblings and infants, and inconvenient location for the visit.

Adult↗
PubMed1986

Effect of chronic heat load during pregnancy on birth weight, behaviour and body composition of Australian feral goat kids.

Six feral does maintained at elevated ambient temperatures (27.2 degrees - 34.8 degrees) for the last half of pregnancy had rectal temperatures (Tr) elevated by 0.4 degrees above 10 unstressed does. Heat-stressed twin-bearers were 0.15 degrees hotter than single-bearers and bore lighter kids (1.70 kg), than unstressed does (2.24 kg) while singles were less affected (2.22 kg versus 2.28 kg). Birth weight was more highly correlated (P less than 0.001) with the daily minimum Tr of the dam than with the mean or maximum Tr. Kids from heated does stood and began sucking as quickly as control kids but during the first six hours scored significantly (P less than 0.01) lower on a scale of general activity. There were no significant differences in body content of nitrogen, fat and ash or, when birth weight was considered, weight of hide, CNS and liver.

Animals↗
PubMed1979

Folded skin associated with underlying nevus lipomatosus.

A mentally retarded female infant had abnormalities of the feet, hemiplegia, and an underlying nevus lipomatosus. To our knowledge, this represents the second reported case of marked folding of the skin; with an underlying nevus lipomatosus; this case demonstrated an association of this cutaneous disorder with multiple defects, including chromosomal abnormalities, which have not been previously reported.

Child, Preschool↗
PubMed1977

Long chain fatty acid inhibition of sodium plus potassium-activated adenosine triphosphatase from rat heart.

Long chain fatty acids were found to inhibit (Na+ + K+)-ATPase prepared from rat heart. Unsaturated and polyunsaturated fatty acids were more inhibitory than saturated fatty acids with myristic acid being the most inhibitory saturated fatty acid tested and linoleic the most inhibitory unsaturated fatty acid. As an example of fatty acid modification of the enzyme, inhibition of (Na+ + K+)-ATPase by oleate was examined. When compared to ouabain, inhibition of (Na+ + K+)-ATPase by oleate was found to be similar in that both were dependent on K+ concentration, but, in contrast to the almost instantaneous inhibition by ouabain, oleate inhibition was a slow process requiring over 20 min incubation at 37 degrees to produce maximum inhibition. Inhibition of rat heart (Na+ + K+)-ATPase by oleate was found to be readily reversible by washout. In the presence of albumin an oleate/albumin molar ratio greater than 7.5 was required for inhibition to occur. The activity of rat heart (Na+ + K+)-ATPase had a temperature optimum above 40 degrees and a discontinuous Arrhenius' plot with a transition temperature of 25 degrees. In the presence of oleate, however, the enzyme's optimum temperature decreased to below 40 degrees, the activation energy of the reaction at temperatures below 25 degrees was lowered from 24.7 kcal/mol to 12.6 kcal/mol and the enzyme had a linear Arrhenius' plot. The possibility of in vivo inhibition of cardiac (Na+ + K+)-ATPase under conditions of elevated fatty acids is discussed.

Adenosine Triphosphatases↗
PubMed1976

Surface topography of the Bacillus stearothermophilus ribosome.

The surface topography of the intact 70S ribosomes and free 30S and 50S subunits from Bacillus stearothermophilus strain 2184 was investigated by lactoperoxidase-catalyzed iodination. Two-dimensional polyacrylamide gel electrophoresis was employed to separate ribosomal proteins for analysis of their reactivity. Free 50S subunits incorporated about 18% more 125I than did 50S subunits derived from 70S ribosomes, whereas, free 30S subunits and 30S subunits derived from 70S ribosomes incorporated similar amounts of 125I. Iodinated 70S ribosomes and subunits retained 62-78% of the protein synthesis activity of untreated particles and sedimentation profiles showed no gross conformational changes due to iodination. The proteins most reactive to enzymatic iodination were S4, S7, S10 and Sa of the small subunit and L2, L4, L5/9, L6 and L36 of the large subunit. Proteins S2, S3, S7, S13, Sa, L5/9, L10, L11 and L24/25 were labeled substantially more in the free subunits than in the 70S ribosome. Other proteins, including S5, S9, S12, S15/16, S18 and L36 were more extensively iodinated in the 70S ribosomes than in the free subunits. The locations of tyrosine residues in some homologous ribosomal proteins from B. stearothermophilus and E. coli are compared.

Electrophoresis, Polyacrylamide Gel↗
PubMed1974

Intracellular protein breakdown in thermophilic and mesophilic fungi.

1. The rate of protein breakdown was determined on growing and non-growing cultures of thermophilic and mesophilic fungi. 2. In growing cells protein breakdown was negligible. 3. In non-growing cells the breakdown rate of total protein varied between 5.2%/h and 6.7%/h. These values were found to be dependent on both the temperature of the protein breakdown assay and the temperature of growth of the organism. 4. The rate of breakdown of soluble protein in thermophilic fungi was 9-15%/h whereas the rate in mesophilic fungi for the soluble protein fraction was only 4%/h.

Aspergillus↗