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Biomedical subjects

H Müller

Publications and source records attributed to H Müller.

At least 721 records · Page 40Linked to original sources
PubMed1982

[Recovery from anesthesia with enflurane and neuroleptanesthesia. Quantitative assessment with the aid of psychopathometric tests].

Methodological differences between techniques used to examine recovery from anesthesia prevent direct comparison of results. Also psychological tests are not validated for certain anaesthesiological considerations. The two tests presented here combine ease of use for a bedside test with the advantage that the results are expressed as absolute values of cerebral depression. Based on Wieck's concept of symptomatic or functional psychosis they were developed to document the course of neuropsychiatric illness. Their application following anaesthesia seems to be justified as anaesthesia may be regarded as being a deliberately induced functional psychosis characterized by extremely compressed dynamics. The target of a first pilot study were 60 patients following neuroleptanalgesia with and without antagonization with naloxone, or enflurane anaesthesia, respectively. Psychopathometric evaluation yielded an obvious post-operational decline in the SKT-scores which clearly remained below the pre-operative values even after three hours. All the patients revealed a severe transient syndrome after thirty minutes, a moderate syndrome after one and two hours and a mild syndrome after three hours. The only exception were patients antagonized with naloxone who initially showed an improvement but who afterwards did not behave any better. Neither duration of anaesthesia nor total dose of drugs administered showed any significant correlation to the extent of postoperative cerebral depression. Being uninfluenced by moderating variables such as age and IQ, the psychopathometric tests presented here seem to provide a sensitive and reliable method for quantification of recovery from anaesthesia.

Adolescent↗
PubMed1982

[Hemodynamic and respiratory effects of tramadol during nitrous oxide-oxygen-artificial respiration and in the postoperative period].

The haemodynamic effects of intravenous tramadol (two different doses: 0.75 mg/kg b.w. or 1.5 mg/kg b.w.) during artificial ventilation with oxygen and nitrous oxide were investigated in 20 female patients before the start of operation. Haemodynamic changes were characterized by a moderate increase in blood pressure concerning systemic as well as pulmonary circulation. These effects were due to a rise in peripheral and pulmonary vascular resistance. Application of tramadol immediately after the end of operation (n = 20:10 patients following neuroleptanalgesia and 10 patients after anesthesia with halothane) led to a slight antagonizable decrease in respiratory frequency and respiratory amplitude as well as increase in paCO2. While during conventional pain-therapy no haemodynamic and respiratory side-effects of tramadol could be demonstrated, the typical qualities of opiates (postoperative ventilatory embarrassment) and unspecific adrenergic effects have to be taken into account after use of high doses for anaesthetic purposes.

Anesthesia↗
PubMed1982

Quantitative determination of suprofen in human plasma and urine by fully automated high-performance liquid chromatography.

A sensitive, specific and rapid determination of suprofen in human plasma and urine by fully automated high-performance liquid chromatography is described. Plasma or urine samples are directly injected into the liquid chromatograph, which consists of commonly available modules. Manual sample cleanup procedures as well as the addition of an internal standard are not needed. Using 20 microliter-aliquots, the detection limit is lower than 0.05 microgram/ml and the calibration ranges from 1.0 to 100 micrograms/ml are linear for both, spiked samples and references. The recovery is 99.2% for plasma and 99.7% for urine samples.

Autoanalysis↗
PubMed1981

Synthesis and processing of in vitro and in vivo precursors of the vacuolar yeast enzyme carboxypeptidase Y.

The biosynthesis of carboxypeptidase Y, which is located in the lysosome-like vacuole of Saccharomyces cerevisiae, has been studied in vitro in a cell-free translation system from wheat germ and in vivo in intact spheroplasts. When a wheat germ system was programmed with yeast RNA, a translation product was immunoprecipitated by anti-carboxypeptidase Y antibodies, which had a slightly smaller molecular weight (Mr = 59,000) on sodium dodecyl sulfate-polyacrylamide gel electrophoresis than the authentic glycoprotein (Mr = 60,000). In the presence of dog pancreatic microsomal membranes, an additional cross-reacting translation product of Mr = 68,000 was formed, which in contrast to the 59,000-dalton form was not susceptible to digestion by externally added proteinases, suggesting its segregation within the microsomal vesicles. The observed increase in molecular weight may be consistent with a core glycosylation of the translocated protein. During a pulse-chase labeling of spheroplasts, the antibody initially precipitated a form of carboxypeptidase Y, which co-migrated on sodium dodecyl sulfate gels with the 68,000-dalton in vitro translation product. Following a chase of 60 min, this early labeled immunoreactive protein was completely converted into the authentic enzyme (Mr = 60,000) and is therefore coincident with the in vivo precursor of carboxypeptidase Y previously described (Hasilik, A., and Tanner, W. (1978) Eur. J. Biochem. 85, 599-608). These data suggest that a vacuolar yeast enzyme is synthesized via a cotranslational segregation of its nascent polypeptide chain within the endoplasmic reticulum giving rise to a proenzyme, which is further processed in vivo into the vacuole-located mature enzyme.

Carboxypeptidases↗
PubMed1981

On the mechanism of vesicle release from ATP-depleted human red blood cells.

The release of spectrin-free vesicles from ATP-depleted human red blood cells (Lutz et al. (1977) J. Cell. Biol. 73, 548) can be considered the final step of a shape change from discocytes to echinocytes. The study of physical and chemical properties of released vesicles suggests that vesicle release is not merely a consequence of charge alterations within either monolayer of the budding membrane. Fresh membranes and released vesicles have within experimental error the same sialic acid content per surface area and the same electrophoretic mobilities. Vesicle release cannot be stimulated by doubling the charge density on the outer monolayer by means of a phospholipase D-treatment, but correlates with a breakdown of polyphosphoinositides to diacylglycerol on the inner monolayer. This breakdown does not lead to a significant change in the negative charge density on the inner monolayer, because an increased phosphatidate content compensates for this alteration. Furthermore, polyphosphoinositide breakdown and diacylglycerol production are not the rate-limiting step in vesicle release from ATP-depleting red blood cells. This is evident from the fact that 10 mM EDTA inhibits vesicle release to 75% without affecting polyphosphoinositide breakdown and diacylglycerol production. Hence, diacylglycerol formation may be sufficient for membrane budding as suggested earlier (Allan et al. (1976) Nature 261, 58), but vesicle release requires a second, as yet unidentified process.

Adenosine Triphosphate↗
PubMed1981

Immunochemical studies on catabolite inactivation of phosphoenolpyruvate carboxykinase in Saccharomyces cerevisiae.

Phosphoenolpyruvate carboxykinase (EC 4.1.1.49) from Saccharomyces cerevisiae was purified to homogeneity. The enzyme is composed of four subunits of Mr = 64,000. Specific antibodies against phosphoenolpyruvate carboxykinase were raised in rabbits and purified by affinity chromatography. Phosphoenolpyruvate carboxykinase is rapidly inactivated when glucose is added to cells starved for carbon (Haarasilta, S., and Oura, E. (1975) Eur. J. Biochem. 52, 1-7; Gancedo, C., and Schwerzmann, K. (1976)( ARch. Microbiol. 109, 221-225). In the present study this inactivation has been analyzed by immunochemical techniques. It was found that the loss of catalytic activity is paralleled by a decrease in cross-reacting material which suggests degradation of the enzyme. In the absence of glucose the enzyme is degraded very slowly, which indicates that glucose-induced inactivation cannot simply be due to repression of enzyme synthesis in the presence of a rapid rate of degradation. Experiments with a proteinase-deficient mutant showed that proteinase B, carboxypeptidase Y, and carboxypeptidase S are not involved in the inactivation system.

Antibodies↗
PubMed1981

[Treatment of the polytraumatic shock (author's transl)].

The survival rate of patients with multiple injuries can be improved by means of the modern possibilities of rescue services. The timespan preceding treatment can be shortened. A consequent symptomatic treatment of hemodynamic and respiratory disorders plays an important role. A causal therapy of the impaired permeability of the capillaries, which causes the shock, is possible only to some extent. The therapy should concentrate on the solution resulting from shock.

Blood Transfusion↗
PubMed1981

Maxillofacial deformities in neurofibromatosis.

Four patients with neurofibromatosis are described: all displayed the feature of an increased depth of the sigmoid notch probably due to an expansive growing neurofibroma of the inferior alveolar nerve. One patient had a local neurofibroma with an invasive growth pattern and only this patient shows hyperplasia of the bone. The suggestion is made, contrary to what is found in the literature, that only the invasive growth type gives rise to hyperplasia and this type is always concomitant with a localized form of neurofibromatosis.

Adolescent↗