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H METZGER

Publications and source records attributed to H METZGER.

At least 19 recordsLinked to original sources
PubMed1964

RECOMBINATION OF ANTIBODY POLYPEPTIDE CHAINS IN THE PRESENCE OF ANTIGEN.

Conditions were developed by which the separated H and L chains of gamma(2) globulins recombined to form four-chained molecules in good yields. In the absence of antigen, anti-2,4-dinitrophenyl (anti-DNP) H chains randomly reassociated with a mixture of antibody and non-specific gamma(2) globulin L chains. In the presence of a specific hapten, however, the antibody H chains preferentially interacted with the anti-DNP L chains. Antibody H chain-antibody L chain recombinants formed in the presence of hapten were more active than the corresponding recombinants formed in the absence of hapten. Speculations are made regarding the possible mechanisms and biological significance of these effects.

Aminocaproates↗
PubMed1963

BINDING CAPACITY OF REDUCTIVELY FRAGMENTED ANTIBODIES TO THE 2,4-DINITROPHENYL GROUP.

Mild reductive cleavage of the polypeptide chains of antibodies to the 2,4-dinitrophenyl group has been carried out according to the methods of Porter and co-workers. The addition of epsilon-2,4-dinitrophenyllysine permitted almost complete retention of the antibody binding capacity upon transfer to IM propionic acid. Fractionation of the chains showed that the hapten was specifically bound to the A and not the B chains, and that about 30 percent of the B chains were bound to A in the presence of the bound hapten. The results confirm the conclusion of Porter and ca-workers that the A chain is the principal component of the antibody site, but suggest that the B chain may also contribute to the site.

Antibodies↗