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H Madsen

Publications and source records attributed to H Madsen.

At least 19 recordsLinked to original sources
PubMed2006

Genetic polymorphisms in the genes encoding human interleukin-7 receptor-alpha: prognostic significance in allogeneic stem cell transplantation.

Interleukin-7 (IL-7) is essential for T-cell development in the thymus and for the maintenance of peripheral T cells. IL-7 signals through IL-7R, that consists of the gammac-chain and an alpha-chain. Sequencing of IL-7Ralpha has revealed the existence of four single nucleotide polymorphisms (SNPs) (+510C/T, +1237 A/G, 2087T/C and +3110A/G), which all give rise to amino-acid substitutions. The aim of the present investigation was to evaluate the significance of IL-7Ralpha SNPs for the outcome in allogeneic stem cell transplantation (SCT). IL-7Ralpha polymorphisms were determined in 195 recipient and donor pairs from either matched sibling donors or matched unrelated donors (MUD). Genotyping of 173 normal controls was performed in parallel. In MUD transplants, the +1237 genotype of the donor was associated with survival after SCT, the mortality being highest and intermediate for the GG and AG genotypes, respectively (P = 0.023). This pattern was more pronounced with respect to treatment-related mortality (P = 0.003), while IL-7Ralpha genotypes were unrelated to the risk of relapse of leukaemia. The IL-7Ralpha +1237 genotype of the recipient and the genotypes of the other three polymorphisms, were not significantly associated with the outcome of SCT. These findings suggest that the IL-7Ralpha polymorphisms may be of importance for treatment-related mortality after SCT.

Case-Control Studies↗
PubMed2006

The schistosome intermediate host, Bulinus nyassanus, is a 'preferred' food for the cichlid fish, Trematocranus placodon, at Cape Maclear, Lake Malawi.

Over the last decade, transmission of the schistosome parasites of humans has increased in parts of Lake Malawi, possibly because over-fishing has led to a decline in the numbers of molluscivorous fish. The stomach contents of 51 wild-caught Trematocranus placodon from the Cape Maclear area of Lake Malawi have now been investigated and compared with the field abundance of snail species at two sites, close to where the fish were caught. The snails found in the fish stomachs were identified to genus or species on the basis of their shell fragments and/or opercula. The sizes of the prosobranchs consumed by the T. placodon were determined from their operculum sizes whereas the Bulinus nyassanus ingested by the fish were categorized as small (<4 mm) or large (> or =4 mm) on the basis of their shell fragments. The proportion of the Bu. nyassanus ingested that were categorized as large increased with fish size. Furthermore, the relative abundance of Bu. nyassanus in the guts of the fish was considerably higher than in the field, indicating that the T. placodon had a 'preference' for Bu. nyassanus over the Melanoides species that dominated the snail fauna. Small specimens of Bellamya species were also consumed in a greater proportion than found in field.

Animals↗
PubMed2005

Insights to the minimal model of insulin secretion through a mean-field beta cell model.

The present work introduces an extension of the original minimal model of second phase insulin secretion during the intravenous glucose tolerance test (IVGTT), which can provide both physiological and mathematical insights to the minimal model. The extension is named the mean-field beta cell model since it returns the average response of a large number of nonlinear secretory entities. Several secretion models have been proposed for the IVGTT, and we shall identify two fundamentally different theoretical features of these models. Both features can play a central role during the IVGTT, including the one presented in the mean-field beta cell model.

Animals↗
PubMed2004

Comparison of schistosome transmission in a single- and a double-cropped area in the rice irrigation scheme, 'Office du Niger', Mali.

Rice is becoming increasingly important as a staple food in West Africa and there is a need to increase production to meet the demand and one way to achieve this is to harvest two annual crops. It is possible that such intensified irrigation could affect transmission of schistosomes and this study attempts to compare schistosome transmission in single and double-cropped areas of an irrigation scheme, 'Office du Niger', in Mali. Double cropping has been practised for some years in the Niono area while in the Kolongotomo area still only a single crop is grown. Parasitological surveys conducted in 10 villages (5 from each of these 2 areas) showed that Schistosoma haematobium was less prevalent in the area with single cropping than in the area with double cropping. In order to compare transmission patterns between the two areas, all human-water contact sites around four villages (two from each area) were identified and included in transmission studies. From August 1996 to June 1997, eight surveys were conducted, at intervals of approximately 45 days, in each of these sites to record density of the intermediate hosts, density of infected snails, environmental factors (especially density of aquatic macrophytes), and frequency of human-water contacts. Although the data provide useful information on transmission patterns in Niono and Kolongotomo areas, they fail to demonstrate clear-cut differences in snail population dynamics and transmission patterns between the two areas. A key element in snail habitats in both areas is the abundance of aquatic macrophytes, especially the submerged species. Snail control using environmental modification should focus on controlling these plants in human-water contact sites.

Adolescent↗
PubMed2004

Epidemiology and morbidity of Schistosoma mansoni infection in a fishing community along Lake Albert in Uganda.

Schistosoma mansoni infection, associated morbidity and symptoms were studied in Piida fishing community at Butiaba, along Lake Albert, Uganda, from November 1996 to January 1997. The study revealed that S. mansoni is highly endemic with an overall prevalence of 72%, a mean intensity of 419.4 eggs per gram (epg) faeces (geometric mean for positives only), with 37.8% of males and 33.0% of females excreting over 1000 epg. Prevalence and intensity peaked in the 10-14 year old age group and decreased with increasing age. Females were less heavily infected than males. Differences were also shown between tribes. Diarrhoea and abdominal pain were commonly reported in Piida. However, no clear-cut correlation between intensity of S. mansoni infection and these conditions could be demonstrated, indicating that retrospective questionnaires concerning S. mansoni related-symptomatology are of limited value. Organomegaly, as assessed by ultrasonography, was frequent and hepatomegaly was associated with heavy S. mansoni infection. No correlation was demonstrated between splenomegaly and infection. This study emphasizes that schistosomiasis mansoni is a major public health problem in Piida fishing community and presumably also in many similar fishing communities. These observations call for immediate intervention and can help in planning long-term strategies for sustainable morbidity control.

Adolescent↗
PubMed2003

Observations on the swarming and mating behaviour of Anopheles funestus from southern Mozambique.

BACKGROUND: Control of malaria by the release of genetically modified mosquitoes refractory to transmission is now becoming a possibility. In many areas of Africa, Anopheles gambiae is found together with an equally important vector, An. funestus. Given their sympatry and the likelihood of a similar mating period some aspects of the mating behaviour of An. gambiae s.l. and An. funestus are likely to differ. We therefore attempted to characterise the swarming behaviour of An. funestus and to determine if any aspects of the observed behaviour differed from that recorded for the M form of An. gambiae from São Tomé. METHODS: In March - May 2002 the swarming, mating, house exiting and resting behaviour of Anopheles funestus was studied by direct observation in Mozambique. Swarming males and insects in copula were collected by sweep net. Wing lengths of males collected resting, exiting houses, swarming and mating were measured and the wingbeat frequency distribution of individual insects, in free flight confined inside netting covered paper cups, was also determined. RESULTS: Mono-specific swarms occurred at sunset in relatively open areas close to houses used for resting. Mating pairs were seen 11 +/- 3.7 min after the start of swarming. The number of total pairs observed being inversely proportional to the time difference between the start of swarming and the first pairing. The great majority of females mated before feeding. Male or female size did not appear to affect mating success or other behaviours. During the study, ambient temperatures decreased and female, but not male, wing size increased. At 516 Hz, the flight tone of female An. funestus was similar to the 497 Hz of the local An. gambiae. Males dispersed if light or dark artificial horizontal markers were placed underneath naturally occurring swarms. CONCLUSION: Differential response to markers would be sufficient for swarming in An. funestus and An. gambiae s.l. to occur in distinct sites.

Activity Cycles↗
PubMed2003

Data assimilation in the decision support system rodos.

Model predictions for a rapid assessment and prognosis of possible radiological consequences after an accidental release of radionuclides play an important role in nuclear emergency management. Radiological observations, e.g. dose rate measurements, can be used to improve such model predictions. The process of combining model predictions and observations, usually referred to as data assimilation, is described in this article within the framework of the real time on-line decision support system (RODOS) for off-site nuclear emergency management in Europe. Data assimilation capabilities, based on Kalman filters, are under development for several modules of the RODOS system, including the atmospheric dispersion, deposition, food chain and hydrological models. The use of such a generic data assimilation methodology enables the propagation of uncertainties throughout the various modules of the system. This would in turn provide decision makers with uncertainty estimates taking into account both model and observation errors. This paper describes the methodology employed as well as results of some preliminary studies based on simulated data.

Algorithms↗
PubMed2002

The swarming and mating behaviour of Anopheles gambiae s.s. (Diptera: Culicidae) from São Tomé Island.

The swarming and mating behaviours of the forest cytoform of Anopheles gambiae s.s. were investigated on 194 evenings and 14 mornings between April 1997 and November 1999 in a peri-urban area of the island of São Tomé, West Africa. Males swarmed 2-3 m above markers of horizontal contrast such as those formed between grass areas and footpaths, or bushes. Evening swarms started 2 min before sunset in sheltered sites and a minute or two later in exposed ones. It took approximately 5 mins from the arrival of the first male for the swarm to reach estimated maximum numbers. Mating pairs were first seen 7 min after the start of swarming. Maximum numbers of pairs in copula were observed 8 min later. Up to 270 pairings were seen in the 20 min period before darkness. Removal of males had no effect on the number of females arriving at the swarm. Males were attracted to sounds that approximated the female flight tone but not to filter paper samples of squashed virgin females swung through the swarm. A much-reduced amount of swarming and a single mating were recorded at dawn. The same locations for swarming were used at different times and at different heights by ants, Culicoides sp. and Culex quinquefasciatus.

Africa, Western↗
PubMed2001

[General practitioners' knowledge of and attitude to assessment and treatment of women with urinary incontinence. A questionnaire among general practitioners in Denmark].

INTRODUCTION: Urinary incontinence is a common problem for adult women, and the need for assessment and treatment of incontinence is expected to increase in the future. The aim of this study was to elucidate the general practitioners' (GPs) knowledge about and attitude to women with urinary incontinence. METHODS: A questionnaire was posted to 1700 randomly selected GPs in 1998 and 1999. RESULTS: A total of 1071 (63%) GPs responded at least once. Five hundred (29%) returned the questionnaire both years. About 50% expressed a positive interest in the management of urinary incontinence. Only 24% felt that their knowledge was sufficient to manage incontinence. About 50% and 66% of the GPs would probably refer a patient with stress incontinence or urge incontinence to a specialist. The GPs' proposals for assessment and treatment were mainly consistent with good clinical practice. There were only minor changes in knowledge and attitude from 1998 to 1999. DISCUSSION: GPs' interest in urinary incontinence is moderate and management is characterised by a high referral rate to a specialist. Most GPs. consider their knowledge to be inadequate. There is need for education to ensure sufficient knowledge and to change the attitude, so that first-line assessment and treatment of urinary incontinence is carried out in general practice.

Adult↗
PubMed2001

Drug prescribing among Danish children: a population-based study.

OBJECTIVES: To characterise the pattern of drug prescribing in Danish children below 19 years of age. METHODS: We used the Odense Pharmacoepidemiologic Database to identify all individual prescriptions for 0- to 19-year-olds in the County of Funen, Denmark, in 1998. The drug consumption was analysed by the defined daily dose methods and anatomical chemical classification system, using analytical templates for individual-based drug utilisation statistics. RESULTS: Of the examined population, 52.6% had one or more prescriptions issued. Overall, 10% of the children accounted for 67.5% of drug prescriptions. Approximately 40% of the children had a prescription issued before they were 1 year old. In 1- to 2-year-olds, 85% received a prescription. Thereafter, the 1-year prevalence of drug use declined to 40%. The total drug consumption was similar between boys and girls until the early teens; thereafter the girls had a prescription issued twice as often as boys. In all age groups, respiratory drugs dominated and drugs used in dermatology was the second largest group. The third largest group was systemic anti-infective agents for children below 12 years of age, whereas it was psychotropic drugs among the oldest children. CONCLUSION: There is the same skewness of drug consumption among Danish children as among Danish adults, but the types of medication differ. Drug use is most extensive among children below 2 years but drug prescribing was considerable in all age groups.

Adolescent↗
PubMed2001

Antibacterial properties of human amnion and chorion in vitro.

OBJECTIVE: The purpose of the present study was to explore the direct effects of amnion and chorion on bacterial growth in vitro including the antibacterial spectrum. Chorioamniotic membranes were obtained under sterile conditions from 13 healthy women undergoing elective cesarean section at term. Likewise, chorioamniotic membranes were obtained from 10 healthy women with spontaneous vaginal delivery at term. Five strains of Hemolytic streptococci group B (GBS) were tested and one clinical isolate of the following species or bacterial groups: Hemolytic streptococcus group A, Staphylococcus aureus, Staphylococcus saprophyticus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter calcoaceticus and Lactobacillus species. Bacteriological media included (1) blood-agar medium; (2) a transparent agar medium for submerged cultures; and (3) a nutrient broth medium. RESULTS: An inhibitory effect of fetal membranes against a range of bacteria was found. Consistent results were obtained in experiments with cultures on agar and cultures suspended in agar (membranes from eight women in both studies). In experiments with liquid cultures (seven women) only chorion showed a marginal inhibitory effect. All strains were inhibited, but the most pronounced inhibition was obtained for streptococcus group A, S. aureus and S. saprophyticus by both chorion and amnion. CONCLUSION: This study demonstrated an inhibitory effect of the fetal membranes on a diverse panel of bacteria

Agar↗
PubMed2001

Sensory changes during the ovulatory phase of the menstrual cycle in healthy women.

This study compared the pain sensitivity in healthy women at the abdomen and lower back (presumed referral areas of menstrual pain), thigh and arm (control areas), in the menstrual, ovulatory, luteal and premenstrual phases of confirmed ovulatory cycles, with that of males. The pressure pain threshold (PPT) and pinch pain threshold (PiPT) was determined by an electronic pressure algometer, heat pain threshold (HPT) by a contact thermode and tactile threshold (TT) with von Frey hairs. The abdominal PPT was significantly lower in females in all menstrual phases as compared to the control sites ( p<0.0007). The abdominal and lower back HPT was significantly lower in females in all menstrual phases compared with control areas, and to the sites in males ( p<0.002). The TT was significantly reduced in females compared with males ( p< 0.013). There was no difference in the PiPT between females and males. In males, the HPT, PPT and TT were not different within any site. During the ovulatory phase, the HPT was significantly reduced at the abdomen and the PPT at the back compared with the menstrual, luteal and premenstrual phases (p<0.0002). There were no within-menstrual phase variations in the PiPT and TT at any site, or for the HPT and PPT at the control areas. The reduced thresholds in menstruating women may be due to the presence of latent uterine algogenic stimuli, and the increased levels of oestrogen and leuteinizing hormone at ovulation may enhance nociception by acting both at the peripheral and central level, resulting in the hypersensitivity changes at the abdomen and lower back areas.

Abdominal Pain↗
PubMed2001

Fluvoxamine inhibits the CYP2C9 catalyzed biotransformation of tolbutamide.

OBJECTIVE: Our objective was to examine the interaction between fluvoxamine and tolbutamide to confirm that fluvoxamine inhibits CYP2C9. METHODS: The study was carried out as an open, randomized, crossover design with 14 healthy participants. In period A, all volunteers took 500 mg of tolbutamide orally. In period B, the volunteers were randomly assigned to one of two groups. Each group took either 150 mg or 75 mg of fluvoxamine a day for 5 days (day -3 to day 2). The groups then took 500 mg of tolbutamide as a single dose (day 0). In both periods, blood and urine were sampled at regular intervals. Plasma was analyzed for tolbutamide, and urine was analyzed for tolbutamide and its two metabolites, 4-hydroxytolbutamide and carboxytolbutamide by means of HPLC. RESULTS: During treatment with fluvoxamine, there was a statistically significant decrease in the median of the total clearance of tolbutamide, from 845 mL/h to 688 mL/h, among the volunteers who received 75 mg/d. There was a reduction that reached borderline statistical significance in the group that received 150 mg/d of tolbutamide. The clearance by means of 4-hydroxytolbutamide and carboxytolbutamide was significantly reduced in both groups (ie, from 901 mL/h to 318 mL/h in the group that received 150 mg of tolbutamide per day and from 723 mL/h to 457 mL/h in the group that received 75 mg of tolbutamide per day). Thus there was a tendency toward a more pronounced inhibition of the 4-hydroxylation during treatment with 150 mg/d of fluvoxamine compared with 75 mg/d, but the difference was not statistically significant. CONCLUSION: Fluvoxamine is a moderate inhibitor of CYP2C9 in vivo.

Adult↗
PubMed2001

Bulinus nyassanus is an intermediate host for Schistosoma haematobium in Lake Malawi.

At Cape Maclear on the Nankumba Peninsula, close to the southern end of Lake Malawi, Schistosoma haematobium is highly prevalent in the local people and many tourists become infected with this parasite each year. A 'Bilharzia Control Programme' was initiated in this area in August 1998, as a development collaboration between the Government of Malawi, the Danish Agency for Development Assistance (Danida), and the Danish Bilharziasis Laboratory. Although Bulinus globosus is a known host for S. haematobium, B. nyassanus has not previously been incriminated as an intermediate host. However, schistosome-infected B. nyassanus were discovered in surveys to identify transmission sites on the peninsula. Experimental infections of wild-caught B. nyassanus with S. haematobium proved successful and S. haematobium eggs were found in hamsters experimentally exposed to cercariae retrieved from schistosome-infected, field-collected B. nyassanus. These are remarkable observations since, although there are very few reports of diploid members of this species group being experimentally infected with S. haematobium, B. nyassanus is a diploid member (2n = 36) of the truncatus/tropicus group. Bulinus nyassanus is probably responsible for transmission in Lake Malawi, along rather exposed shorelines, devoid of aquatic macrophytes, with a substrate of sand or gravel.

Animals↗
PubMed2000

New challenges in schistosomiasis control in Morocco.

Cases of schistosomiasis were first detected in 1914. However, there is strong evidence that it was prevailing in the southern part of Morocco long before then. As reported from different African countries, over the last three decades, the development of irrigation has led to the spread of the disease to the north and centre of the country. Thereafter, a national programme of schistosomiasis control was launched by the Ministry of Health in 1983. The programme was based on case detection and treatment, snail control using chemicals, and health education. It has succeeded in reducing the prevalence and intensity of infection to a level low enough to allow an elimination programme to be launched in 1994. The aim is to reach a complete elimination of the disease by the year 2004 in all provinces affected. Though substantial progress was made since the programme started, there is a potential risk of outbreaks in many affected provinces. Therefore, an integrated approach including measures against the intermediate host is needed to reach the goal of elimination. The present paper sheds some light on the features of schistosomiasis in Morocco and presents an evaluation of environmental methods of control of Bulinus truncatus in Tessaout Amont and Akka oasis irrigation schemes. The role of community involvement in planning and implementing environmental measures against the snail intermediate host in modern and traditional irrigation schemes is also discussed.

Animals↗
PubMed2000

Schistosoma haematobium in Morocco: moving from control to elimination.

In this article, Hammou Laamrani and colleagues summarize the Moroccan schistosomiasis control programme and discuss the challenges ahead for schistosomiasis elimination. In 1994, a programme was initiated by the Moroccan Ministry of Health to eliminate schistosomiasis from Morocco by the year 2004. In 1997, this objective had been achieved in three out of 20 affected provinces. This article discusses the background and strategies of this programme, as well as the achievements, the problems encountered and the challenges ahead, along with suggestions as to how to reach the goal of elimination of urinary schistosomiasis in Morocco, and possibly elsewhere in Africa.

Animals↗