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H Mashimo

Publications and source records attributed to H Mashimo.

25 records · Page 2Linked to original sources
PubMed1986

Conservation and diversity in the class I genes of the major histocompatibility complex: sequence analysis of a Tlab gene and comparison with a Tlac gene.

The thymus leukemia (TL) antigens, encoded by class I genes in the Tla subregion of the major histocompatibility complex (MHC), are cell surface molecules expressed on thymocytes of certain strains of mice and on certain T-cell leukemias. In order to study the fine structure and interrelationships of genes of the Tla subregion, a Tla-specific probe was isolated from the TL-encoding T13c gene of BALB/c mice (Tlac haplotype). The probe hybridized with two Tla genes in the Tlac haplotype (T13c and T3c) and with only one in the Tlab haplotype (T3b). Examination of this subset of Tla genes (T3b, T3c, and T13c) by restriction enzyme analysis and oligonucleotide hybridization studies confirmed that T3b is the allele of T3c and that T3c and T13c may have arisen by duplication. The T3b gene, while not transcribed in the tissues of the TL- strain C57BL6, was shown to be transcriptionally active in the TL-expressing leukemic cell line ERLD derived from that strain. The T3b gene was cloned and its complete DNA sequence was determined. These data permit complete comparison of two Tla-region genes, T3b and its homologue T13c, and allow us to conclude that these genes show extraordinarily high sequence conservation, in contrast to alleles of the H-2K- and H-2D-region genes. Comparison of T3b with other class I sequences in the H-2 and Qa subregions suggests that the T3-subset genes are the most divergent from other class I genes.

Alleles↗
PubMed1981

Human T cell surface antigens bearing a structural relationship to HLA antigens.

Three cell surface antigens that are structurally related to the human major histocompatibility antigens (called HLA antigens) have been characterized from the leukemic T cell line MOLT-4. One antigen is a glycoprotein of Mr 49,000 recognized by two monoclonal antibodies. OKT6 and NA1/34, and is associated with a Mr 12,000 subunit that crossreacts serologically with beta 2-microglobulin but can be distinguished from it by sodium dodecyl sulfate/polyacrylamide gel electrophoresis. A second antigen, defined by the monoclonal antibody OKT10, is a Mr 46,000 protein associated with a small subunit distinct from beta 2-microglobulin. The OKT10 antigen is not restricted to T cells and is found on all T and B lymphoblastoid cell lines tested. The third protein is a beta 2-microglobulin-associated glycoprotein of Mr 43,000 that is serologically distinct from the OKT6 (NA1/34), OKT10, and HLA antigens. It is found on some, but not all, T cell lines but is absent from any other hematopoietic cell lines tested.

Antigen-Antibody Complex↗