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Biomedical subjects

H McEwan

Publications and source records attributed to H McEwan.

4 recordsLinked to original sources

Use of telephone enquiries to a microbiology laboratory as a proxy measure of reporting efficiency.

AIMS: To assess the efficacy of a bacteriology service in respect of the time interval between collection of specimen and receipt of final report using the number and type of incoming telephone enquiries as a proxy measure. METHODS: For three months, all incoming telephone enquiries regarding the results of bacteriology specimens were monitored. Specimen type, date of sampling, the sender's location and the reason for making the telephone enquiry were recorded. RESULTS: The number of telephone enquiries made during the study was 1170, about 5% of the total number of samples received. Most enquiries related to results of urine cultures. These accounted for 33.9% of the calls, but only 5% of the total number of urines cultured. Enquiries relating to blood cultures were the next largest group accounting for 14.9% of calls, but 11% of the blood cultures received resulted in a telephone enquiry. The most frequent reason for making the telephone call was that the sender had not received the final report. CONCLUSIONS: Clinicians may have unrealistic expectations of the time taken to examine a specimen. A requirement for reporting sterile blood cultures after a shorter incubation period was found, and the value to patient management of earlier reporting of negative urine samples was identified.

Bacteriology

Collagenolytic activity of crevicular fluid from pericoronal gingival flaps.

Fluids from normal and inflamed gingival flap tissues, overlying partially erupted lower third molars, were incubated on reconstituted collagen gels and examined for collagenase activity. Collagenase activity was observed only in fluids originating from tissues with pericoronitis. However, when fluids from clinically normal tissues were treated with NaSCN, some also showed collagenase activity. Only the fluids from normal-looking tissues with a history of pericoronitis showed collagenase activity after NSCN treatment. No enzyme activity was observed in treated or untreated fluids from normal tissue from patients with no history of pericoronitis. It was concluded that the fluid collagenase activity originated from the adjacent gingival tissue because (1) the activity of the untreated fluid observed in this study seemed to parallel the acitivity of the adjacent gingival tissue, based on our observations in a previous study; (2) NaSCN affected the fluid collagenase in the same way that others found it to affect gingival tissue collagenase; and (3) the disc gel electrophoretic pattern of the breakdown products of collagen produced by the GCF enzyme was similar to that produced by gingival tissue collagenase and differed from that produced by a bacterial collagenase reported to exist in human gingival fluid.

Adolescent