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H Meyer-Rienecker

Publications and source records attributed to H Meyer-Rienecker.

At least 19 recordsLinked to original sources
PubMed1991

Immunoadsorption and plasma exchange in multiple sclerosis: complement and plasma protein behaviour.

Three groups of patients suffering from acute attacks or progressive multiple sclerosis (MS) are under investigation. First results revealed remarkable clinical improvements of patients with acute attacks in the groups treated by therapeutic plasma exchange (TPE) and immunoadsorption (IA). Only slight or no improvements were seen in the patients of the control group treated only with steroids. Plasma protein levels (IgG, IgM, IgA, fibrinogen) were considerably reduced in patients of the TPE group after each treatment procedure as expected. The same holds true concerning the total hemolytic capacities (THC) of the complement of the classic (CP) and the alternative (AP) pathway. On the other hand in the IA group only slight decreases of plasma proteins (about 20%) were observed, but the behaviour of THC's were quite similar than those seen in the patients of the TPE group. The THC decreases in both groups can be explained by removal of all complement factors (TPE group) or by the adsorption of single factors (IA group) of both complement pathways according to earlier in vitro investigations. The THC decreases in patients of both groups suffering from acute MS attacks could mean an "antiinflammatory" effect and could--at least partially--contribute to the clinical improvements of these patients.

Blood Proteins↗
PubMed1991

Classical and modern methods of cerebrospinal fluid analysis. Report on the First All-German Symposium of the Society for Laboratory Medicine (FRG) and the Study Group for CSF Analysis and Clinical Neurochemistry of the Society for Psychiatry and Neurology (formerly GDR) in Marburg a. d. Lahn, October 5-6, 1990.

The aim of the symposium was to prepare an inventory of cerebrospinal fluid (CSF) analysis used in Germany, and to evaluate them in comparison with modern methods. From the large field of CSF analysis, four main topics were selected, all related to the practical application of the methods. The following conclusions were drawn: Classical techniques of cytodiagnosis are clinically important. Therefore, manual and mechanized techniques must be further improved with respect to counting, collection, and differentiating of CSF cells. As cytokines and complement factors are early mediators of diverse processes in CNS, highly sensitive techniques must be developed for their routine analysis e.g. in CNS inflammation. Recent efforts to detect specifically viral and bacterial agents (e.g. by polymerase chain reaction, Particle Counting Immuno-Assay, Enzyme Immuno-Assay) or antibodies (e.g. affinity-mediated immunoblot, specific antibody index) in CSF must be continued in order to develop definite and practicable assays for daily routine. For the detection of intrathecally produced antibodies, qualitative procedures appear to be more reliable then quantitative ones, provided that the former are highly sensitive and specific.

Animals↗
PubMed1988

Incidence of multiple sclerosis: a periodic or stable phenomenon.

For diseases of unknown aetiology, the question as to whether the incidence is constant or variable is very important. A study on multiple sclerosis in a defined northern area of the German Democratic Republic showed a prevalence of 68.6 and an incidence rate of 3.0. Retrospective and prospective investigations concerning an observation period of 22 years revealed cyclic periods (6-7 years) of high incidence rates (up to 4.5) interrupted by shorter intervals (4-5 years) with low rates (about 1.8). The differences (1963-1968 vs. 1969-1973, 1974-1978 vs. 1979-1983) are significant. In accordance with the findings of Kurtzke et al. on a cyclic outbreak of multiple sclerosis in the Faroes and Iceland, our results are considered to be a consequence of environmental factors, such as epidemic viral infections.

Adolescent↗
PubMed1979

[Aspects of immunologic diagnosis of brain tumors. Results with tumor-associated membrane antigens and the electrophoretic mobility test (author's transl)].

In cell-mediated immune processes the products of antigen-sensitive lymphocytes are detectable as charge-changing lymphokines by means of the cell electrophoretic mobility method (EM test). the improvement of the method concerns the measuring procedures (indicator particle and automation) and the application of specific antigens. The employment of special membrane antigens (3 M KCl extracts) from tissue of normal brain and brain tumors is decisive for further differentiating results. The application of tumor-associated membrane antigens (TAA) from various kinds of brain tumors shows some diagnostically important reaction patterns. Using a common tumor antigen, the TAA-X3 mixture, a (1) characteristic result of general significance for neoplasms can be obtained; in applying the different TAA of brain tumors the findings in EM test point out an (2) organospecific reactivity and/or a (3) histogenetic reaction pattern. The distinct neuroepithelial and mesodermal brain tumors produce predominantly an organospecific reaction and only partly a histogenetical profile. For this type of reactivity to the TAA in brain tumors, a series of factors depending on neuroimmunological specialities are important.

Adult↗
PubMed1976

[The macrophage electrophoretic mobility LAD test--a diagnostic method for multiple sclerosis (author's transl)].

With the MEM test (Field) one can establish a cellular immune reaction becarus the sensitized lymphocytes release the macrophage slowing factor (MSF) upon interaction with the appropriate antigen. A macrophage migration inhibition was detected in some neurological diseases with destruction of the parenchyma. The modification MEM-LAD (linoleic acid depression) test made further differentiation possible in the 146 neurological patients and normals. The reduction of macrophage mobility inhibition was 94.7 + 4.7% in multiple sclerosis (MS) cases as compared with that of normals of 55.1 + 3.7% and of other neurological diseases of 47.8 + 7.1%. There were no significant differences due to the course and duration of the disease or to immunosuppressive therapy. The pathogenically important results in relatives of MS patients with values between the MS and normal group (78.5 + 0.7%) in mothers suggested a familial (genetic) disposition. The same value was found in a monozygotic twin of an MS patient. The results in the children studied showed that besides the endogenic metabolic component the aetiopathogenically important exogenic factors can operate early in life. In correlation with the principle of the MEM-LAD test the suppressive action of linoleic acid can result in a further therapeutic concept.

Adolescent↗
PubMed1976

Mixed lymphocyte reaction in multiple sclerosis.

The mixed lymphocyte reaction (MLR) as measured by the macrophage electrophoretic mobility (MEM) test is markedly reduced between unrelated multiple sclerosis (MS) patients. This is not the case with other neurological diseases (OND). Within an MS family, the MLR between the propositus and members of the family falls into the low (MS type) range or into the normal or OND range. Those members who give the low results are those who have a MEM-LAD test result of about 77 per cent, i.e. halfway between that of normal and MS. There is thus a parallelism between the anomalous response to linoleic acid and an unexpectedly low MLR with known MS lymphocytes. Lymphocytes from apparently normal children who have a high (MS-type) linoleic acid depression result take part in an MLR with MS cells, as if they were themselves true MS cells. Some possible implications these findings may have for the pathogenesis of MS are discussed.

Adolescent↗
PubMed1976

[Cerebrospinal fluid diagnosis of multiple sclerosis].

Starting from the knowledge accumulated with respect to the etiopathogenesis and the components of immunoreaction a minimal to maximal program by steps has been developed for the cerebrospinal fluid (CSF) in multiple sclerosis (MS). It is based on fundamental methods (I), special supplementary methods (II), and relatively specific immunological methods (III). For MS five possible conditions of the CSF could be determined from alterations of cells and variations in protein: a (1) typically complete and (2.) typically incomplete immunoreactive encephalomyelitic (encephalitic) syndrome, a (3.) nonspecific CSF-syndrome of low degree and less typical character (in the sense of an acute or subacute irritation syndrome) an (4.) atypical syndrome of a considerable degree, and a (5.) normal condition of the cerebrospinal fluid. The significance of immunoreactive cerebrospinal fluid syndromes to the diagnostic criteria of multiple sclerosis as well as further relatively disease-specific methods (such as the MEM test and MSF assay) of determining cellular immunity, are discussed.

Cell Count↗
PubMed1976

[Basic principles and application of cerebrospinal fluid immunoelectrophoresis].

The basic principles, prerequisites, and limitations of the immunoelectrophoresis of the cerebrospinal fluid are discussed. Major emphasis has been placed on the characteristics of normal and pathological immunoelectropherograms of the liquor cerebrospinalis as well as on the results of studies of inflammatory and neuroimmunological diseases. The detection of a barrier profile, the method of determining special qualitative alterations of immunoglobulins of the liquor cerebrospinalis due to neuroimmunological diseases, and additional applications are discussed in detail. The procedure has been critically reviewed because the results obtained are, for the most part, of a general and nonspecific character and the method cannot be standardized adequately and also does not yield sufficient quantitative data. The advantages (differentiated qualitative changes) and disadvantages (technique troubles) are pointed out.

Blood-Brain Barrier↗
PubMed1975

[Use of the macrophage-electrophoretic-mobility test in the diagnosis of tumours of the central nervous system (author's transl)].

The macrophage-electrophoretic-mobility (MEM) tests of Field and Caspary demonstrates the macrophage-slowing factor (MSF) produced by specifically sentised blood lymphocytes after incubation with the homologous antigen. Besides other diseases with a cellular immune reaction like multiple sclerosis malignant tumours show a reduction of the macrophage mobility in the cell electrophoresis. A significant inhibition in the MEM- test was found in 30 out of 33 tumours of the CNS. Glioblastomas, astrocytomas, spongioblastomas, ependymomas, neurinomas, meningiomas, and a myxofibroma showed a positive result (more than 10% inhibition). The results in ectodermal tumours (pituitary adenoma, craniopharyngioma) differed; secondary brain tumours showed the greatest inhibition.

Adolescent↗