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Biomedical subjects

H Middleton

Publications and source records attributed to H Middleton.

27 records · Page 2Linked to original sources
PubMed1991

The influence of maternal antibody and age of calves on effective vaccination against Leptospira interrogans serovar hardjo.

Twelve seronegative cows were vaccinated with an experimental bivalent Leptospira interrogans serovars hardjo and pomona vaccine late in their first pregnancy. Calves born of these dams were divided into 4 equal groups that received this vaccine at 4, 6, 10 and 18 weeks of age, respectively. Before vaccination the group geometric mean titres of maternally-derived circulating antibodies ranged from 2 to 25 for the microscopic agglutination (MA) test and 3 to 35 for enzyme-linked immunosorbent assay (ELISA) using a serovar hardjo outer envelope antigen. Post-vaccination peak titres were 645 to 1612 for MA and 562 to 1037 for ELISA, respectively. Calves vaccinated at the youngest age, had the highest pre-vaccination circulating maternal antibody titres, but showed the smallest rise in post-vaccination antibody titres. Circulating maternal antibody was detected in calves up to 13 weeks of age. All immunised calves were protected against a virulent challenge with serovar hardjo type Hardjobovis, regardless of their age or maternally-derived antibody titres. These findings indicate that calves as young as 4 weeks old, vaccinated in the presence of maternally-derived antibody, can be fully protected against homologous virulent challenge.

Age Factors↗
PubMed1990

Factors influencing general practitioners' management of psychosocial and physical problems: a study using case vignettes.

The reactions of a random sample of 72 Oxfordshire general practitioners to case vignettes illustrating psychosocial and physical presentations were assessed with a set of attitude rating scales. Two main underlying factors appeared to influence responses. The first reflected positive attitudes to both physical and psychosocial problems, and was associated with postgraduate experience in psychiatry and with older age. The second, reflecting measures of flexibility and responsibility for outcome, was associated with younger age and not having received vocational training. Other characteristics of the practitioners and their practices were not significantly related to these factors, and there was considerable unexplained variance. In addition, certain sex differences emerged: men general practitioners rated their ability to manage cases more highly than did their women colleagues, while women practitioners experienced more anxiety. These findings suggest that personal traits and qualities may remain a stronger determinant of general practitioners' reactions to patients' problems than formal training and qualifications.

Adult↗
PubMed1987

The neuroendocrine effects of oral imipramine.

The release of growth hormone, prolactin and cortisol following oral imipramine was studied in nine fit young men. Imipramine 100 mg, but not 40 mg, led to reliable rises in the circulating levels of all three hormones in the majority of subjects. These responses are likely due to the enhancement of central noradrenergic and serotonergic function as a result of reuptake inhibition. The safety, sensitivity and reliability of these responses make imipramine 100 mg orally a potentially valuable neuroendocrine challenge test.

Administration, Oral↗
PubMed1987

Evaluation of an enzyme-linked immunosorbent assay for the detection of Corynebacterium pseudotuberculosis infection in sheep.

Two enzyme-linked immunosorbent assays to measure antibody to the cell wall antigen (C-ELISA) and toxin antigen (T-ELISA) of C. pseudotuberculosis were evaluated on serum from 6 separate groups of sheep. For sheep naturally infected with C. pseudotuberculosis the sensitivity and specificity of the C-ELISA was 76% and 73% respectively and for the T-ELISA 67% and 77% respectively. For sheep slaughtered one year after artificial infection the sensitivity of both tests was greater than or equal to 83% and the specificity greater than or equal to 72%. For sheep slaughtered 4 months after artificial infection the specificity of both tests was less than 30% while the mean sensitivity was 85%. The C-ELISA in conjunction with the T-ELISA detected 92% of sheep with lung lesions.

Animals↗
PubMed1984

A case of Klinefelter's syndrome with acquired hypopituitarism.

The association of Klinefelter's syndrome (KS) and hypopituitarism has been described in previous isolated case reports, in most of which the hypopituitarism was partial. A case is described of a man with KS who acquired hypopituitarism in adult life. The diagnosis of KS was made at the age of 25 years on standard laboratory tests when he presented with failure of ejaculation and typical clinical features. Thirteen years later he presented with anaemia and further regression of secondary sexual characteristics. All aspects of anterior pituitary function were abnormal. The cause of the hypopituitarism was not determined, but may have been post-traumatic.

Adult↗