Biomedical subjects
Publications and source records attributed to H N Guttman.
A peptide isolated from rats habituated to a sound stimulus has been given the structure (see article) Glu-Ala-Gly-Tyr-Ser-Lys-OH. A synthesis of this compound afforded a product that different from the natural material on the basis of chromatographic and physiological comparisons. The proposed sequence must therefore be in error.
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Inducible malic enzyme (l-malate:NAD oxidoreductase [decarboxylating], EC 1.1.1.39) was isolated from Lactobacillus plantarum and purified about 100-fold with 27% yield of the original activity. Kinetic studies with the purified malic enzyme yielded the following results: pH optimum, 7.6 to 8.0; K(m) for l-malate, 0.38 mM; K(m) for NAD, 0.072 mM; and K(m) for MnCl(2), 0.048 mM. It was shown that this enzyme was inhibited by high concentrations of substrate and nicotinamide adenine dinucleotide (NAD), indicating it may be regulated by substrate or NAD. Molecular weight of 130,000 +/- 10,000 was determined by Sephadex gel filtration and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The isoelectric point, determined by isoelectrofocusing, was 4.3 at 7 C. Isoelectrofocusing also resolved three active peaks which focused at pH 4.19, 4.31, and 4.40.
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Two independent research groups replicate alteration of dark preference to dark avoidance by mice injected with synthetic scotophobin, a pentadecapeptide.
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Simple washing of live Euglena gracilis cleans the pellicle of external coating material. When these cleaned cells are viewed with a scanning electron microscope, organelle outlines can be seen through the thinned pellicle. To view organelles directly, fixed Euglena cells are gently broken first or first frozen and then broken as part of their preparation for scanning electron microscopy. Subsequent viewing at magnifications of x 5,000 to x 21,000 reveals organelles, storage granules, endoplasmic reticulum, and nuclear pores, while retaining great depth of focus.
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