Biomedical subjects

H Ohnogi

Publications and source records attributed to H Ohnogi.

6 recordsLinked to original sources

PubMed1996

The nucleotide sequence of human acylamino acid-releasing enzyme.

The nucleotide sequence of a cDNA coding for the human acylamino acid-releasing enzyme (AARE, also known as acylpeptide hydrolase) [EC 3.4.19.1] subunit has been determined. The amino acid sequence of human AARE subunit deduced from its cDNA nucleotide sequence showed a high degree of identity (91.5%) with both the corresponding proteins from the pig and the rat. The AARE cDNA shows 99.2% identity with a 3.3 kb cDNA transcribed from a locus (DNF15S2) on the short arm of human chromosome 3, whose deletion is associated with small cell lung cancer, taking into consideration that the sequence of the 3.3-kb cDNA previously reported was caused by misreading.

Amino Acid Sequence↗

PubMed1993

Antithrombotic effect and reperfusion by low molecular weight heparin in a canine model of coronary artery thrombosis.

The lability of an intracoronary thrombus, which is common in the case of unstable angina, indicates the presence of endogenous fibrinolysis. To prove our hypothesis that suppression of coagulation facilitates the thrombolytic process, low molecular weight heparin (LMWH) was given intravenously to dogs with intracoronary thrombi induced by copper coils. LMWH caused reperfusion in this model and suppressed the formation of intracoronary thrombi, thus supporting our hypothesis.

Animals↗

PubMed1993

[The cognition of intra-familial kinship terms among junior high school students].

A cognitive task concerning intra-familial kinship terms was imposed on 125 junior high school students (male 57, female 68). Forty-six questions were read successively and subjects were required to answer each item. These questions consisted of two types; one required the subjects to identify various familial relationship (e.g. "my uncle") from everyday description (e.g. "my parent's brother"), and the other presented relationship through legal degree of relationships (e.g. "my parent's parent's male child, other than my parent"), some questions of both types containing redundant information concerning gender. The main results were as follows. (1) Correct percentages on lineal relatives regardless of question type were very high. (2) High correlation between related gender-differentiated kinship terms was observed. (3) While questions employing ordinary expressions yielded higher correct percentages than those expressed in legal terms, redundant information reduced correct percentages when combined with ordinary expression.

Adolescent↗

PubMed1992

The primary structure of porcine aminoacylase 1 deduced from cDNA sequence.

A cDNA encoding the complete amino acid sequence of aminoacylase 1 (N-acylamino acid aminohydrolase, ACY-1) [EC 3.5.1.14], a dimeric metalloprotein having two Zn2+ in the molecule, which catalyzes the deacylation of N-acylated L-amino acids except L-aspartic acid, has been isolated from porcine kidney lambda gt10 cDNA library and sequenced. From sequence analysis of the cDNA and the N- and C-terminal amino acid analyses of the purified protein, it is deduced that porcine kidney ACY-1 consists of two identical subunits (M(r) 45,260), each of which consists of a single chain of 406 amino acids with acetylalanine at the N-terminus. A cDNA encoding porcine liver ACY-1 was also cloned. The amino acid sequence deduced from the nucleotide sequence of the cDNA from porcine liver was identical to that deduced for porcine kidney ACY-1. Northern blot analysis suggested that ACY-1 is more highly expressed in kidney than in liver. Comparison of the amino acid sequence of porcine ACY-1 with those of other Zn2+-binding metalloenzymes showed no significant homologies in either the overall sequence or the consensus sequences for the metal binding sites. This indicates that ACY-1 is a new type of metalloprotein.

Amidohydrolases↗

PubMed1986

Four-year-old children's visual attention in direct and observational learning.

The study was designed to compare direct learning (learning by performing) with observational learning (learning by observing) on a memory task that assessed 4-year-old children's selective attention. 17 pairs of kindergarteners of the same sex (aged 3 yr., 8 mo. to 4 yr., 4 mo.) participated in a central-incidental memory task. Data from a retention test indicated that the performers' mean central learning score was higher than their mean incidental score, while the observers showed no difference between their central and incidental learning scores, performers had a higher mean central learning score than observers. On the other hand, the frequency of the subjects' overt visual attention to the task in their learning phase was also counted. Both observers and performers showed high frequency of visual attention.

Attention↗

PubMed1985

Kindergarteners' selective attention in direct and observational learning.

The present study was designed to compare direct learning (learning by performing) with observational learning (learning by observing) in a version of central-incidental memory task. A 2 X 2 factorial design, containing methods of participation (performer, observer) and game-trials (1 and 5 games) was used. 80 kindergarteners (6 yr. old) were divided into four groups. In the performer and observer conditions, central recall scores were higher than incidental recall scores, five-game groups showed more central recall than one-game groups, five-game groups showed more incidental learning than one-game groups, and recognition scores were higher than recall scores. The results suggested that the observers' attention to stimuli may be inferred to be very similar to the performers', for the observers' score patterns of the two measures were similar to those of the performers.

Association Learning↗