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Biomedical subjects

H Ohoka

Publications and source records attributed to H Ohoka.

26 records · Page 2Linked to original sources
PubMed1988

[Combination chemotherapy with cisplatin and UFT malignant tumors of the head and neck--experimental study of human nasopharyngeal carcinoma (A2L/AH) xenografted to nude mice].

Combination therapy with cis-platinum (CDDP) and UFT was examined with human nasopharyngeal carcinoma (A2L/AH) implanted in nude mice. The tumor growth of A2L/AH was inhibited in the group administrated UFT 20mg/kg, but was not in 10mg/kg group in comparison with the control group. An inhibition rate (IR) of the tumor growth was 80.2 and 21.3% respectively. The group received CDDP (5mg/kg, q7d X 3, 2mg/kg, q7d X 3 and 1 mg/kg, qd X 6) by i. p. injection, resulted in 75.2, 37.4 and 23.1% inhibitions respectively. While, the response rate in the group treated with CDDP (1 mg/kg, qd X 6) and UFT (10mg/kg) showed a synergistic effect (IR; 66.3%) which was higher than in the group administrated CDDP (2mg/kg, q7d X 3) and UFT (10mg/kg) (IR; 58.3%).

Administration, Oral↗
PubMed1988

Thomsen-Friedenreich antigen in bladder cancer tissues detected by monoclonal antibody.

Monoclonal antibodies against Thomsen-Friedenreich antigen (T-Ag) were obtained by the hybridoma technique. The expression of T-Ag in 73 specimens of bladder cancer was examined by the immunofluorescence method using these monoclonal antibodies. Seventeen (53%) of grade I, 18 (44%) of grade II and 5 (50%) of grade III were diffusely stained with anti-T. Of the T-negative tumors, 14 (44%) of grade I and 13 (32%) of grade II showed positive staining after neuraminidase treatment (cryptic T-positive) while only 1 (10%) of grade III was cryptic T-positive. On the contrary, only 1 case (3%) of grade I was cryptic T-negative while 10 (24%) of grade II and 4 (40%) of grade III (were. The T-antigen expression and the histological grades correlated significantly (p less than 0.05). Twenty-eight (70%) of the T-positive cases and 9 (60%) of the cryptic T-negative cases recurred while only 3 (11%) of the cryptic T-positive cases did (p less than 0.01). The monoclonal antibodies were more useful than peanut lectin (PNA) or conventional polyclonal antibodies on the detection of T-antigen.

Antibodies↗
PubMed1985

Thomsen-Friedenreich antigen in bladder tumors as detected by specific antibody: a possible marker of recurrence.

TUR specimens of non-invasive transitional-cell carcinomas were examined for their expression of Thomsen-Friedenreich (T) antigen by indirect immunofluorescence staining using rabbit IgG antibody which was raised with desialated glycophorin. Nine (45%) out of 20 tumors of low grade (Grade I and II), and 5 (56%) of 9 tumors of high grade (III) were diffusely stained with anti-T (T-positive), whereas T-antigen in normal tissue was cryptic and stained only after neuraminidase treatment (cryptic T-positive). Of the T-negative tumors, 9 (45%) of the low grade but only one of the high grade tumors, were stained positively for the cryptic T-antigen. The rest of the tumors were devoid of the cryptic T-antigen. Eighty percent of both Tumors expressing T-antigen and those lacking the cryptic T-antigen recurred within two years. Recurrence was not influenced by initial histological grade.

Antibodies↗
PubMed1984

In vitro protein synthesis using D-amino acids and its evolutionary significance.

The in vitro incorporations of D-Trp or -Ala into peptides were investigated using a crude system. Although none of aminoacylation to the former was observed under the present condition, the latter was utilized for peptide synthesis at the same rate as the opposite enantiomer. The results of analyzing the peptides showed that the peptides, which were synthesized with either D-, L- or DL-Ala as the substrate, were composed of mostly similar molecular size. The study of incorporations of radioisotopes (14C-D- and 3H-L-Ala) into peptides also indicated that D-Ala was by no means inferior to L-Ala for the substrate. Basing upon the present experiment, the evolutionary significance for utilizing D-amino acids is discussed.

Amino Acids↗