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Biomedical subjects

H Okabe

Publications and source records attributed to H Okabe.

At least 19 recordsLinked to original sources
PubMed1979

High and low density lipoprotein cholesterol in myocardial and cerebral infarction.

High density lipoprotein (HDL) and low density lipoprotein (LdL) cholesterol levels were measured in fasting blood samples from 950 healthy subjects and 188 aged patients by a new simple method. The HDL-cholesterol levels and HDL/LDL-cholesterol ratios are significantly higher in females than in males. In the healthy subjects, there are slight decreases in the levels of HDL-cholesterol and HDL/LDL-cholesterol ratio with aging in both sexes. The patients with myocardial infarction had significantly lower HDL-cholesterol levels and HDL/LDL-cholesterol ratios as compared to those of the group without infarction. On the contrary, no differences in total lipoprotein cholesterol levels were observed in the patients with cerebral infarction. The results, obtained in respect of electrocardiographic findings after the isoproterenol stress test, suggest that the HDL-cholesterol levels and HDL/LDL-cholesterol ratios may be related not only to the established myocardial infarction, but also to the presence of coronary atherosclerosis and stenosis.

Adult↗
PubMed1979

Improved method for simultaneous determination of cholesterol in high- and low-density lipoproteins.

A previously described procedure for simultaneous determination of cholesterol in high- and low-density lipoproteins (HDL and LDL) [Clin. Chem. 24, 1504--1508 (1978)] has been improved by using centrifugation instead of ultrafiltration. Addition of NICl2 (2.0 mmol/L) to the reagent produced a good separation of HDL from the complex of low- and very-low-density lipoprotein-heparin-Ca2+ on centrifugation at 3000 rpm for 15 min. Replicate analyses for high-density lipoprotein cholesterol by the present method demonstrated the following intra-assay precision: mean = 389 mg/L, SD = 11 mg/L, CV = 2.8%. The present (y) and original (x) methods gave results that agreed reasonably well (n = 50, r = 0.960, y = 1.0x + 0.9). The enzymic method for HDL- or (HDL + LDL)-cholesterol after the separation of their fractions gave erroneous results, in particular in the cases of hyperbilirubinemic sera and in (HDL + LDL) fractions.

Cholesterol↗
PubMed1978

A portion of the feline leukaemia virus genome is not endogenous in cat cells.

Although viral sequences closely related to feline leukaemia virus are represented in multiple copies in cellular DNA of all domestic cats, a specific fraction was present only in the virus-infected cells. This fraction was detected by viral cDNA enriched by a prior absorption of a total complementary DNA (cDNA) transcript with normal cat liver DNA. The recycled cDNA hybridized well with the cellular DNA of virus-infected cells, but to a lesser extent with DNA from uninfected cat cells. The probe was used to differentiate virus-positive from virus-negative tumour tissues of cats. The same approach with cDNA of another endogenous feline virus, RD114, failed to show any difference between a virus-infected cell line and normal cells, including both virus-inducible and non-inducible lines.

Animals↗
PubMed1978

Virology and histopathology of the trigeminal ganglia of Americans and Japanese.

Herpes simplex virus in the trigeminal ganglia of humans was studied in Philadelphia, Pennsylvania, United States of America, and in Kyoto, Japan. The prevalence of recurrent herpes labialis and of clinically latent herpes simplex virus within trigeminal ganglia was determined in inhabitants of the two cities. In addition, a comparison was made of the prevalence of mononuclear cell infiltration in the trigeminal ganglia of Americans and Japanese. Recurrent herpes labialis was found to be significantly less common in the Japanese city than in the American city. Herpes simplex virus was rescued less commonly from the trigeminal ganglia of cadavers in Japan than in America. The difference was significant. The frequency of mononuclear cell infiltration in the trigeminal ganglia of Americans and Japanese is not significantly different. These observations, as well as previously reported serological studies, suggest that despite the ubiquitous nature of herpes simplex virus in America and Japan, the Japanese have less clinically overt disease caused by this virus.

Adolescent↗
PubMed1978

Waldenström's macroglobulinaemia with Ig E M-component.

A case of Waldenström's macroglobulinaemia with Ig E M-component was presented. A 76-year-old Japanese woman had a history of the operation for mammary carcinoma. Although neither lymphadenopathy nor hepatosplenomegaly was present, the immunohaematological findings typical for Waldenström's macroglobulinaemia were observed in this patient. Coexistence of the Ig M and Ig E M-components was recognized in the patient's serum protein. Positive reaction for FITC-conjugated anti-epsilon or rhodamine-conjugated anti-mu was noted in proliferating lymphoplasmacytic cells of the patient's bone marrow, but these reactions were not simultaneously observed in a single cell. The finding suggests that two separate populations of M-component-producing cells were present.

Aged↗
PubMed1978

Molecular mechanisms involved in the differential expression of gag gene products by clonal isolates of a primate sarcoma virus.

Clonal isolates of an early passage stock of woolly monkey sarcoma virus (WSV) have been shown to code for different numbers of woolly monkey helper leukemia virus gag gene products. In the present report, the molecular mechanisms responsible for their differential expression of gag gene products have been analyzed. Three WSV RNA genomes were shown to possess sedimentation coefficients consistent with the differences demonstrated in their allotments of helper viral sequences. The WSV variant (WSV clone 9) that expressed no detectable proteins was shown to contain the largest amount of helper viral information. Moreover, there was no additive hybridization of the WLV complementary DNA probe by RNA of this WSV clone and that of a WSV clone coding for several gag gene products. These results suggest that the lack of expression of gag gene products by WSV clone 9 is not due to a major deletion of helper viral gag gene sequences. Similar levels of WLV-specific RNA were demonstrated in cells nonproductively transformed by each WSV clone, arguing that the ability to express gag gene proteins was not related to the magnitude of viral RNA transcription. Taken together, the results are most consistent with a mechanism by which small deletions or point mutations in the genomes of some WSV variants result in premature termination of translation or synthesis of immunologically nonreactive gag gene proteins. The present findings have implications concerning the effects of evolutionary selective pressures on helper viral genetic information in mammalian transforming viruses.

Base Sequence↗
PubMed1978

Reiteration frequency of feline type C viral genomes in homologous and heterologous host cell DNA.

Differences in the multiplicity of proviral sequences of two feline endogenous viruses, RD-114 and FeLV, in homologous and heterologous host DNA were examined by viral cDNA hybridization using cellular DNA fractionated with respect to reiteration frequency. The endogenous proviral DNAs in cat cells were fractionated predominantly with intermediate-repeated sequences, while RD-114 proviral DNA in virus-infected human cells was fractionated together with unique-sequence DNA. These differences were consistent with the results of kinetic analysis using unfractionated DNA.

Animals↗
PubMed1978

Simultaneous determination of serum cholesterol in high- and low-density lipoproteins with use of heparin, Ca2+, and an anion-exchange resin.

Cholesterol concentrations in serum high-density and low-density lipoproteins are simultaneously determined simply, specifically, and rapidly by use of the precipitation method with heparin, Ca2+, and an anion-exchange resin. The isolation of lipoproteins is reproducible, selective, and complete, as judged by electrophoresis on polyacrylamide gel and by immunoelectrophoresis, with use of samples with very-low-density lipoprotein triglyceride concentrations of less than 3.5 g/liter. The precision of the present method is as good (CV, 2.8-3.1%) as that for the method used by the U.S. Lipid Research Clinics (CV 2.0-3.2%). The present method and the heparin-Mn2+ method of the Clinics gave results that agreed reasonably well (for low-density-lipoprotein cholesterol r = 0.935, P less than 0.001; for high-density-lipoprotein cholesterol r = 0.837, P less than 0.001). we also describe the relations between high- or low-density lipoprotein cholesterol and total cholesterol, and between cholesterol concentrations in these two lipoprotein classes.

Adult↗