Biomedical subjects
H P Bauer
Publications and source records attributed to H P Bauer.
Perfusion of the psoas muscle of the rabbit. Metabolism of a homogeneous muscle composed of "fast glycolytic" fibres.
A method was developed for the hemoglobin-free perfusion of the rabbit psoas muscle in situ. This muscle consists almost exclusively of fast-twitch (type IIb) glycolytic fibres and was therefore used as a model of a homogeneous muscle of the glycolytic, metabolic type.
The concentrations of glucose 1,6-bisphosphate and other regulatory metabolites, and the activities of enzymes of the glycogen metabolism in the perfused rabbit psoas muscle.
The following parameters were determined in the rabbit psoas muscle after perfusion in the presence of either insulin, propranolol, or isoproterenol: Concentrations of cyclic AMP, glucose 1,6-bisphosphate, fructose 2,6-bisphosphate, glucose-1-phosphate, glucose 6-phosphate, and fructose-1,6-bisphosphate. Maximum and "regulatory" activities of the enzymes glycogen phosphorylase, glycogen synthase, phosphofructokinase, and histone-phosphorylating protein kinase.
Phosphofructokinase activity and the binding of enzymes to glycogen particles in the perfused psoas muscle of the rabbit.
The activity of phosphofructokinase in the perfused rabbit psoas muscle was investigated after perfusion in the presence of either propranolol or isoproterenol, and after 48 hr starvation. The phosphofructokinase activities were correlated with the concentrations of glucose 1,6-bisphosphate in the muscles. A considerable fraction of enzymes of the glycogen metabolism and of phosphofructokinase was bound to glycogen particles. The extent of binding was not regulated by the glycogen content.