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Biomedical subjects

H P Rusch

Publications and source records attributed to H P Rusch.

At least 19 recordsLinked to original sources
PubMed1985

The beginnings of cancer research centers in the United States.

The first center for cancer research in the United States was started in 1898, and during the first half of the present century 19 additional cancer research centers had their origins. Most of them were initiated with funds from wealthy individuals who had experienced the tragic loss of a close relative. The early years of these centers and anecdotes about some of the early directors of the centers are described.

Cancer Care Facilities↗
PubMed1971

Nuclear acidic protein changes during differentiation in Physarum polycephalum.

A class of acidic nuclear phosphoproteins has been isolated throughout the mitotic cycle and at two points during differentiation in the slime mold Physarum polycephalum. The electrophoretic profiles of these proteins are reproducible and unchanging throughout the mitotic cycle, but reproducible changes occur during differentiation. The proteins are rapidly synthesized after mitosis, and their molecular weights range from 34,000 to 88,000. The proteins rapidly incorporate [(32)P]orthophosphate, and the content of alkali-labile phosphate increases 20 percent during the period after DNA synthesis. The proteins comprise 6.5 percent by dry weight of nuclear material while the DNA comprises about 5.5 percent. These acidic nuclear proteins may have a role in control of gene activity.

Amino Acids↗
PubMed1971

Synthesis and some properties of an actin-like nuclear protein in the slime mold Physarum polycephalum.

A protein was extracted from isolated nuclei of the slime mold Physarum polycephalum which could be labeled with radioactive precursors only during G(2) phase. The native protein was purified by extraction in low-ionic-strength buffer [10 mm tris(hydroxymethyl)aminomethane-hydrochloride] of isolated nuclei and by preparative polyacrylamide gel electrophoresis. It was extracted from isolated nucleoli. Its electrophoretic properties in three different polyacrylamide gel systems, its molecular weight (44,000 +/- 3,000), its precipitability by vincaleucoblastine, a vinca alkaloid, and its aggregation properties suggested that it might be actin. In a direct comparison with slime mold actin purified from the cytoplasm, no difference could be found between the two proteins in all these characteristics. The synthesis of cytoplasmic actin was not found to occur exclusively during G(2) phase. This suggested that nuclear actin was either synthesized independently from cytoplasmic actin or transported into the nuclei exclusively during G(2) phase. The possible role of nuclear actin during intranuclear mitosis is discussed.

Actins↗