Biomedical subjects
Publications and source records attributed to H Parolis.
The primary structure of the acidic capsular antigen of Escherichia coli O8:K25:H9 was shown by monosaccharide analysis, methylation analysis, and by 1D and 2D 1H and 13C NMR spectroscopy to be composed of linear tetrasaccharide repeating units having the structure:
NMR spectroscopy was performed on the depyruvated capsular antigen of E. coli K103 and on the oligosaccharide obtained by depolymerisation of the native polysaccharide with a viral-borne endoglycanase. This capsular polysaccharide is the only one to be co-expressed with O group 101 and joins a small group of unusual capsular polysaccharides which possess pyruvic acid as the only acidic function. The primary structure was shown to be composed of the repeating unit: [formula: see text]
The primary structure of the acidic capsular antigen produced by E. coli K84 was shown by glycose and methylation analysis, and by 1D and 2D 1H and 13C NMR studies of the polysaccharide and an oligosaccharide produced by lithium-ethylenediamine degradation of the polysaccharide, to be comprised of linear hexasaccharide repeating units of the following structure: -->4)alpha-D-GaI pA-(1-->3)-alpha-D-Man p-(1-->4)-beta-D-GIc p-(1-->3)-alpha- D-Glc pNAc-(1-->2)-beta-D-Man p-(1-->3)-beta-D-Man pNAc-(1-->.
The primary structure of the acidic capsular antigen of Escherichia coli O8:K50:H- was shown by glycose analysis, methylation analysis, and one- and two-dimensional 1H and 13C NMR spectroscopy to be composed of repeating linear tetrasaccharide units having the structure: (formula: see text).
The structure of the exocellular capsular polysaccharide expressed by Escherichia coli O8:K48:H9 has been investigated by hydrolysis and methylation analysis, and by NMR spectroscopic studies of the polysaccharide and of the oligosaccharides generated by solvolysis with anhydrous HF. The capsular polysaccharide was shown to have the repeating unit: [formula: see text] where beta-L-Sug p represents 2,3-diacetamido-2,3,6-trideoxy-beta-L- mannopyranose.
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The structure of the capsular polysaccharide produced by Escherichia coli O20:K83:H26 was investigated by one- and two-dimensional 1H and 13C NMR spectroscopy, and by glycose and methylation analysis. The capsular polysaccharide was shown to be comprised of linear pentasaccharide repeating units with the structure: [formula: see text]
The primary structure of the acidic capsular antigen of Escherichia coli O8:K45:H9 was shown by glycose analysis, methylation analysis, and one- and two-dimensional 1H and 13C NMR spectroscopy to be composed of repeating branched tetrasaccharide units having the structure: [formula: see text] The polysaccharide contains the uncommon amino sugar 3-acetamido-3,6-dideoxy-D- galactopyranose, which has not been previously identified in a capsular antigen.
The capsular polysaccharide of Klebsiella K43 has been studied by glycose analysis, methylation analysis, and NMR spectroscopy, and by bacteriophage depolymerisation of the native polysaccharide. Additional evidence for the structure of the repeating unit came from base-catalysed degradation of the methylated polysaccharide, and from NMR spectroscopic analysis of the lithium-degraded polysaccharide and of the oligosaccharide-alditol derived from the repeating unit oligosaccharide obtained from a bacteriophage degradation. The polysaccharide was shown to have the repeating unit: [formula: see text]
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The structure of the capsular antigen of E. coli O8:K102:H- was investigated by methylation analysis, beta-elimination of the methylated polysaccharide, lithium-ethylenediamine mediated degradation, and by 1D and 2D 1H and 13C NMR spectroscopy of the lithium-degraded and native polysaccharides. The capsular antigen was shown to have the following branched pentasaccharide repeating unit: [formula: see text]
The annexed structure of the capsular polysaccharide of Escherichia coli O9:K35:H- (A104a) has been determined by glycose and methylation analysis, base-catalysed degradation, and 1D and 2D NMR spectroscopy on the polysaccharide and the products obtained by bacteriophage-mediated depolymerisation. [formula: see text]
The capsular polysaccharide from Escherichia coli K47 was investigated using mainly methylation analysis and 1H and 13C NMR spectroscopy and shown to have the following repeating unit: [Formula: see text]
The structure of the capsular antigen from Escherichia coli O8:K46:H30 was elucidated by methylation analysis and 1D and 2D 1H- and 13C-NMR spectroscopy, and by methylation analysis, 1D- and 2D-NMR spectroscopy, and FABMS of the oligosaccharide-alditol obtained after dephosphorylation of the polymer with aqueous hydrofluoric acid. The capsular polymer is of the teichoic acid type and has the following repeating unit. [Formula: see text]
The structure of the capsular polysaccharide from Klebsiella K15 has been re-investigated, principally by 1D and 2D 1H- and 13C-NMR spectroscopy of the oligosaccharide-alditol obtained by depolymerisation of the polysaccharide with a viral-borne endoglycanase followed by borohydride reduction of the isolated repeating oligosaccharide. The capsular polysaccharide was shown to have the repeating unit: [Formula: see text]
The primary structure of the O-deacetylated acidic capsular antigen of Escherichia coli O9:K38 was shown by glycose analysis, methylation analysis, and one- and two-dimensional 1H- and 13C-n.m.r. spectroscopy to be composed of repeating linear pentasaccharide units having the structure.
The structure of the exocellular glycocalyx polysaccharide of Pseudomonas fragi ATCC 4973, a bacterium implicated in the spoilage of meat, has been determined using hydrolysis, methylation analysis and 1D- and 2D-n.m.r. spectroscopy. The polysaccharide, which aids in the adhesion of the cells to each other and to the meat tissue, has the regular repeating unit ----4)-3-O-[(R)-1-carboxyethyl]-alpha-D-Glcp-(1----3)-beta-D-ManpNAc+ ++- (1----4)-beta-D-Glcp-(1----. Random partial O-acetylation occurred in some preparations of the polysaccharide.
The structure of the capsular polysaccharide from Escherichia coli O8:K87:H19 was investigated by methylation analysis and by one- and two-dimensional 1H- and 13C-n.m.r. spectroscopy. The repeating unit was shown to be a branched pentasaccharide with the structure (formula; see text)