Purification and characterization of lipoprotein lipase from rat brown fat.
Explore the source record for details and available documents.
Biomedical subjects
Publications and source records attributed to H Pellet.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Phase contrast microscopic observation of the urinary sediment has to be performed on freshly collected urine. This technique, which permits an excellent definition of the cells and the maintenance of intra cellular motility, gives therefore a great accuracy in differential diagnosis, especially for little round cells. Among these, polynuclear granulocytes are easily recognized, because of their lobulated nuclei and of their numerous intra cytoplasmic motile granulations. Lymphocytes and lymphoblasts have round, regular, homogeneous nuclei. Renal tubular cells exhibit eccentric nuclei, with smudged chromatine, and round, refringent cytoplasmic vacuoles. Rapid and easy, this technic should be used for routine microscopic study or urinary sediments.
Explore the source record for details and available documents.
Phase contrast microscopy allows a much better identification of the urinary sediment constituents than conventional bright field microscopy. The urinary sediment of 100 neonates admitted in a care unit has been examined by phase contrast microscopy. In all cases, squamous cells are far more numerous in newborn girls than in boys. An important rate of abnormal sediments has been observed: in 40 patients cytologic symptoms of renal ischaemia have been observed (hyaline or granulous casts and renal tubular cells, but red blood cells are uncommon). Numerous uric acid cristals were persent in 22 newborns, in correlation with a prolonged perinatal hypoxia. A considerable bacteriuria was seen in 21 neonates, without concomitant leucocyturia. In conclusion, only 20% of the investigated neonates had a normal urinary sediment.
Corticosterone is more concentrated in the white and brown fat of the Rats killed after ether and nembutal anesthesia, than by decapitation. Corticosterone in the plasma increases in the same manner. There results show that adipose tissue is a pool of dilution in balance with the circulating corticosterone.
Corticosterone and cortisol are assayed by fluorometry after separation of the corticoids by chromatography on silica gel in the plasma and in the adrenals of golden Hamster brought up in the laboratory. The ratio cortisol : corticosterone is 2.7 in the plasma and 2.8 in the adrenals.
In the Rat brown adipose tissue, the authors have observed by electron microscopy two different cellular aspects : some cells are more dark, they contain numerous glycogen granules, their mitochondrias are pressed close together and the mitochondrial matrix is darker then the cristae ; in the light cells, the mitochondrias are more sparse and moderately electron dense. The two cellular aspects are also observed on thick sections.
The suppression or reduction of the adrenal secretion in the Rat and in the golden Hamster results in a reduction of the weight of brown adipose tissue and of the volume of intra cytoplasmic vacuoles. When corticotropin is given to normal and hypophysectomized animals, the intra cytoplasmic vacuolar system and the corticosterone concentration of brown fat increase. After sham hypophysectomy and short ether intake, a considerable lipidic depletion and a high level of corticosterone are observed. The authors speculate that the by adrenaline. The morphologic responses are much more intense and rapid lipid content of brown adipose tissue is increased by corticotropin and reduced by adrenaline. The morphologic responses are much more intense and rapid in brown adipose tissue than in white adipose tissue.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.