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Biomedical subjects

H Pointner

Publications and source records attributed to H Pointner.

At least 55 records · Page 3Linked to original sources
PubMed1976

Extraction of gastrin from normal porcine pancreas.

Gastrin was extracted from normal porcine pancreas by the method used by Gregory and Tracy for the extraction of gastrin from the antrum. Radioimmuno-assay was used for identification at all stages of the extraction procedure. Gastrin of pancreatic origin was eluted from a sephadex G 50 and a DEAE-collulose column at the soma position as porcine antral gastrin. In disc electrophoresis (7.5% polyacrylamide in tris-glycin buffer) we found a single band which migrated the same distance from the origin as reference gastrin (synthetic human gastrin I). Densitometircally there was also a single band. The eluate of this electrophoretic band produced an increase in gastric acid secretion after intravenous injection in the rat. A dilution series was made from the extracted fraction and radioimmunologically tested with gastrin antibodies. The individual points of the dilution curve corresponded well with the standard calibration curve of gastrin. A certain discrepancy between the small amount of gastrin found by radioimmunoassay (500 pg/g fresh pancreas) and biochemical and biological findings could probably be explained by a different sensitivity of the gastrin antibody of the radioimmunological system to pancreatic gastrin. Our results prove that there is gastrin in the normal porcine pancreas.

Animals↗
PubMed1975

[The measurement of 75selenium in duodenal juice--an advance in the diagnosis of pancreatic function (author's transl)].

The radioactivity in the duodenal juice following the intravenous injection of 75Se-1-selenomethionine was measured in 17 normal subjects and in 14 patients with exocrine pancreatic insufficiency. The mean values of radioactivity were significantly lower during certain periods of the investigation in the patients with pancreatic insufficiency as compared with the normal subjects. The individual values, however, scattered widely and the standard deviations were large. There was a highly significant correlation between the volume of duodenal juice and the 75Se radioactivity, but no significant correlation was established between the duodenal juice radioactivity and bicarbonate or enzyme concentration i.e. specific parameters of exocrine pancreatic function. It is possible that observation period may bring less equivocal results, but this appears of doubtful practicability. Measurement of the 75Se-1-selenomethione radioactivity in the duodenal juice is only an indirect indicator of exocrine pancreatic function. It does not appear to represent any significant advance in the diagnosis of pancreatic insufficiency, since the information provided by these older-established methods is more reliable and is obtained within a similar space of time.

Cholecystokinin↗
PubMed1975

75Se-selenomethionine excretion in bile and pancreatic juice.

The radioactivity in the bile and pancreatic juice of two patients was measured after intravenous injection of 75Se-l-selenomethionine. The concentration of radioactivity in both fluids is almost equal. Thin-layer chromatography shows that the main part of radioactivity in pancreatic juice is due to unbound 75Se-selenomethionine, while free 75Se-selenomethionine is only a minor part of the total radioactivity appearing in the bile.

Bile↗
PubMed1975

Normal serum gastrin levels in patients with liver cirrhosis.

In 28 patients with cirrhosis of the liver, histologically confirmed by liver biopsy, serum gastrin concentrations were determined radioimmunologically afer an overnight fast. Mean value and standard deviation in the patients with cirrhosis (30.1 +/- 19.3 pg/ml) was not found to be significantly different from the mean value established in 275 normal subjects (39.7 +/- 21.3 pg/ml).

Gastrins↗
PubMed1975

[Treatment of exocrine pancreatic insufficiency with fungal lipase (author's transl)].

7 patients suffering from severe exocrine pancreatic insufficiency have been treated with a lipolytic enzyme extracted from Rhizopus arrhizus. Comparing the fungal lipase with a placebo the drug lowered the daily stool weight from 809 g to 443 g on an average, i.e. by 45.2%. The steatorrhea was reduced from 75.6 g/24 h to 32.9 g, i.e. by 56.5%. When incubating the enzyme in vitro in saline solutions of different pH for 1 h the loss of lipolytic activity is 13% at pH 5, 15% at pH 4, and 19% at pH 3. So the enzyme, like fungal proteases, is almost not altered by hydrochloric acid at concentrations found physiologically in the stomach.

Celiac Disease↗
PubMed1975

Hormone-induced diarrhea in man.

In a healthy subject infusions of either secretion or glucagon caused no diarrhea. A combinations of gastrin and glucagon induced severe watery diarrhea immediately after the end of the 3 hour infusion. No diarrhea occurred from the combination of secretin and gastrin.

Adenoma, Islet Cell↗