Biomedical subjects

H Qin

Publications and source records attributed to H Qin.

86 records · Page 5Linked to original sources

PubMed1994

[Protection against experimental hepatic injury by fengxiang yigankang capsule].

Protective effect of Fengxiang Yigankang (FXYGK) capsule against hepatotoxicity induced by CCl4 and acetaminophen (AAP) was studied. It was found that the FXYGK capsule inhibited markedly malonic aldehyde (MDA) formation of liver induced by CCl4 and AAP. It blocked also depletion of reduced form of glutathione (GSH) of damaged liver induced by AAP. In addition, FXYGK could decrease serum alanine aminotransferase levels induced by CCl4 (P < 0.05). The results of histopathological examination showed that the FXYGK capsule (0.45, 0.9 and 1.8 g/kg) could also reduce significantly fatty degeneration of liver (P < 0.05).

Acetaminophen↗

PubMed1994

[Action of Astragalus membranaceus on left ventricular function of angina pectoris].

The action on left ventricular function of Astragalus Membranaceus (AM), a Qi-tonic, in 20 patients with angina pectoris was studied by means of Doppler Echocardiogram (DEC). It showed that cardiac output increased from 5.09 +/- 0.21 to 5.95 +/- 0.18 L/min 2 weeks after AM was administered (P < 0.01), and no improvement of left ventricular diastolic function appeared. Adenosine triphosphatase activity was not inhibited by using AM, which was different from that of digitalis.

Adenosine Triphosphatases↗

PubMed1993

Isolation and characterization of a linear plasmid from the entomopathogenic fungus Ascosphaera apis.

An extrachromosomal DNA plasmid was isolated from both mating types of the entomopathogenic fungus Ascosphaera apis and named pAaL. The subculture in which pAaL was first identified originated from mummified honey bee larvae from an apiary in Wyoming. Very similar, homologous plasmids were found in 9 out of 10 of isolates collected from diverse geographic locations. The plasmid is found inside the mitochondria, has the same buoyant density as mitochondrial DNA in bisbenzamide--CsCl gradients, and does not contain sequences homologous to either mitochondrial DNA or genomic DNA. The plasmid is linear, double-stranded, of 12 kilobase pair, and has a higher copy number than the mitochondrial DNA. Endonuclease and exonuclease digestions suggest that an inverted repeat is probably present at each terminus and that pAaL has two blocked 5' ends, probably due to the presence of terminal binding proteins. Restriction site data showed pAaL to be AT-rich. There were no apparent differences in the growth rate, culture appearance, and reproductive cycle of plasmid-bearing or plasmid-free A. apis isolates. pAaL was stably inherited in the plasmid-free strains, but it was lost in the progeny of crosses and reciprocal crosses between the plasmid-free strain and plasmid-bearing strains. The biological function of this plasmid has not yet been determined.

Ascomycota↗

PubMed1993

Simple microwave and thermal cycler boiling methods for preparation of cervicovaginal lavage cell samples prior to PCR for human papillomavirus detection.

Sample preparation is an important step in the detection of viral DNA by the polymerase chain reaction (PCR) technique. The method used should achieve release of cellular DNA with the minimum of manipulation steps so as to reduce the possibility of contamination. The present report demonstrates that either microwaving or 20 min of boiling in the heating block of a thermal cycler lead to satisfactory results in the detection by PCR of human papillomavirus in cervicovaginal epithelial cell specimens obtained by lavage. Since each of these methods uses only one step the possibility of contamination is greatly reduced compared to the widely used proteins K/nonionic detergent extraction procedure.

Carcinoma in Situ↗

PubMed1993

Intelligence function in children with acute lymphoblastic leukemia after treatment.

In order to evaluate the effects of chemotherapy and central nervous system prophylaxis on the intelligence function of children with acute lymphoblastic leukemia (ALL), 147 patients were divided into 2 groups according to their different treatment regimens. Group A included 75 patients with ALL who were diagnosed and treated from 1981 to 1986. Group B included 72 ALL patients diagnosed and treated from 1988 to 1990. A control group included 73 healthy children who shared the same education and environmental background with the patients. Chinese revisions of Wechsler measures of intelligence quotient were used to estimate verbal IQ, performance IQ and total IQ in the 3 groups. American-produced Systate software was used to analyze the data. Results showed that IQ distribution, verbal IQ, performance IQ and total IQ of group A were obviously lower than those in the control group. Verbal IQ and total IQ of group B were also reduced. Significant differences of verbal IQ and performance IQ as well as total IQ were also found between groups A and B. Multiple regression analysis showed negative correlations between IQ and cranial irradiation dosage, times of intrathecal MTX and time after irradiation.

Antineoplastic Combined Chemotherapy Protocols↗

PubMed1991

Localization of human glandular kallikrein-1 gene to chromosome 19q13.3-13.4 by in situ hybridization.

Humans possess 3 fully characterized kallikrein-like genes. The gene expressed in kidney, pancreas and salivary gland (KLK), and the gene encoding prostate-specific antigen (APS) have been localized to chromosome 19q13.2-qter. The present study describes the localization of the remaining gene, hGK-1, which has highest homology to and a similar tissue specificity of expression as the APS gene. Using a [3H]-labeled probe derived from a hGK-1 genomic clone, we demonstrated hybridization confined to the q13.3 and q13.4 bands of chromosome 19 and suggest that kallikrein genes may possibly be located near the border of these two bands.

Chromosome Mapping↗

PubMed1991

[Case control study on risk factors of cerebral thrombosis].

In order to explore the pathogenic risk factors or protective factors of cerebral thrombosis, 1:1 matched case control study was done in 110 cases diagnosed by computerized tomography (CT). Both cases and controls were selected from several provincial and municipal hospitals in Jinan, Shandong Province, China. Every case was matched with one control on sex, race, age, occupation, residential area, educational level and economic status. 31 factors were analysed and 9 risk or protective factors were found by univariate analysis and multiple stepwise regression. Using the same 9 factors as independent variables, conditional logistic regression was performed and 4 factors were confirmed as pathogenic risk factors or protective factor of cerebral thrombosis (alpha = 0.01). They are high blood pressure (beta = 3.46, OR = 7.57), abdominal skinfold thickness (beta = 3.21, OR = 3.77), familial aggregation of stroke (beta = 2.25, OR = 12.64) and high level HDL2-C (beta = -2.99, OR = 0.16). Moreover, reliability of collective data and control o: bias were evaluated and discussed.

Adult↗

PubMed1990

Localization of human cardiac beta-myosin heavy chain gene (MYH7) to chromosome 14q12 by in situ hybridization.

The genes coding for each human cardiac myosin heavy chain (alpha-MHC and beta-MHC, MYH6 and MYH7, respectively) are tightly linked and the alpha-MHC gene has been assigned to chromosome 14. In order to provide a more precise regional localization, in situ hybridization experiments were carried out using a 3H-labeled probe derived from a beta-MHC genomic clone. The results demonstrated that the human cardiac MHC genes are located within the q12 band of chromosome 14.

Chromosome Banding↗

PubMed1990

[Diagnosis value of lateral pharyngeal roentgenography in snoring disease].

In this paper, X-ray findings of the lateral pharynx before uvulopalatopharyngoplasty in 14 cases of snoring disease were studied. The results are as follows. There is less than 5 mm in anterior-posterior airway of pharyngeal cavity (13 cases), and 8 mm (1 case), in diameter. Minimum 2 mm, average 4.4 mm. There is less than 25 mm length of uvula (7 cases), 28-35 mm (7 cases). Minimum 22 mm, average length is 27.9 mm. Shape of uvula is thick and long (12 cases), thick and short 1 case, thin and long 1 case. The etiology, abnormal X-ray finding and X-ray examination of the lateral pharynx, etc. in snoring disease have been discussed.

Adult↗

PubMedDate not supplied

Determinants of estimated insulin resistance and beta-cell function in Indian, Creole and Chinese Mauritians. The Mauritius NCD Study Group.

Associations with insulin resistance and islet beta-cell function have been studied cross-sectionally in a population-based sample of 4278 Mauritians comprising Asian Indian, Creole and Chinese subjects. Insulin resistance and beta-cell function were estimated by a computer solved model based on fasting plasma glucose and insulin concentrations. Insulin resistance increased with declining glucose tolerance, whereas beta-cell function was highest in subjects with impaired glucose tolerance (IGT) and lowest in those with non-insulin-dependent diabetes mellitus (NIDDM). Indian subjects had the highest beta-cell function, while ethnic differences in insulin sensitivity were less marked. This may indicate that deranged beta-cell function rather than insulin resistance is the primary determinant of hyperinsulinaemia and glucose intolerance in Asian Indians. beta-Cell function declined with age more sharply than did insulin sensitivity, suggesting that the age-related decline in glucose tolerance is primarily related to loss of beta-cell function. Body mass index, waist/hip ratio, physical inactivity and female sex were independently associated with insulin resistance and beta-cell function. Subjects with a family history of diabetes had increased insulin resistance, irrespective of glucose tolerance. This or similar models may have application in longitudinal population-based studies which seek to determine the relative contributions of insulin resistance and beta-cell function to the aetio-pathogenesis of NIDDM.

Adult↗

PubMedDate not supplied

Conjugation of unprotected trisuccin, N-[tris[2-[(N-hydroxyamino)carbonyl]ethyl]methyl]succinamic acid, to monoclonal antibody CC49 by an improved active ester protocol.

For the conjugation of the trihydroxamate bifunctional chelating agent N-[tris[2-[[N-(benzyloxy)amino]-carbonyl]ethyl]methyl]succinamic acid (trisuccin, 1) to antibodies, we originally used the corresponding 2,3,5,6-tetrafluorophenyl active ester followed by the postconjugation removal of the benzyl protecting groups by catalytic hydrogenation. It was of interest to us to design a conjugation protocol capable of incorporating deblocked hydroxamates into peptides and proteins. Reported procedures that were expected to be compatible with the functionalities present in trisuccin were used with no success, as judged by the lack of ability of the products to radiolabel with 188Re. A simple conjugation method was then developed utilizing the o-nitrophenol (ONP) activated ester of the unprotected trisuccin, N-[tris[2-[(N-hydroxyamino)carbonyl]ethyl]methyl]succinamic acid, 3, which eliminates the need for the postconjugation deblocking. An assay for indirect estimation of the active ester content, based on the concentration of its decomposition byproduct, ONP-OH, was developed. Comparison of the indirectly estimated concentrations with those obtained directly from purified products showed > 90% accuracy for this assay. This procedure has the advantage of rapidly using the unpurified active ester, eliminating the possibilities of its decomposition through solvolysis or self-condensation by the unprotected hydroxamate functions. A colorimetric assay was developed for estimation of the number of ligands per molecule of protein. This assay and the fact that all conjugates consistently radiolabeled with 188Re show that this procedure conjugated the unprotected hydroxamate ligands to the CC49 monoclonal antibody. These results indicate the potential applicability of this technique to conjugation of unprotected hydroxamate derivatives with other proteins and peptides.

Antibodies, Monoclonal↗

PubMedDate not supplied

Reassignment of human renin gene to chromosome 1q32 in studies of a (1;4)(q42;p16) translocation.

The human renin gene (REN) has been assigned to chromosome 1q42. Linkage studies are, however, inconsistent with this localization. We therefore reexamined the question of the location of REN using a patient whose distal chromosome 1q arm was translocated to chromosome 4 [(1;4)(q42;p16)]. In situ hybridization using a 3H-labelled REN probe demonstrated hybridization signals confined to the q32 band of chromosome 1, with radioactivity in the translocated 1q42 region being similar to the low levels along all other chromosomes.

Chromosome Mapping↗

PubMedDate not supplied

Recombinant vaccinia virus expressing cytokine GM-CSF as tumor vaccine.

The efficacy of a recombinant vaccinia virus (rvv-GM-CSF) expressing the granulocyte macrophage colony stimulating factor (GM-CSF) as tumor vaccine was evaluated in the murine B16-F10 melanoma model. The vaccine was prepared by infection of irradiated tumor cells with rvv-GM-CSF. Control vaccine was B-16 cells infected with a recombinant vaccinia virus expressing Escherichia coli beta-galactosidase (rvv-lacZ). Pre-vaccination of naive C57BL/6 mice later inoculated with tumor cells and treatment of mice bearing tumors with GM-CSF vaccine inhibited tumor development and prolonged survival. Lung metastasis of B-16 was also inhibited by treatment with GM-CSF vaccine. The vaccine effects appeared to be tumor cell specific. The efficacy of the vaccine was comparable to a retroviral vaccine (MFG-muGM-CSF) in this system. The vaccine was also effective when rvv-GM-CSF was directly injected into the tumor. These data suggest that this vaccine approach has potential for use in cancer treatment, especially for patients with easily accessible tumors.

Animals↗

PubMedDate not supplied

Recombinant vaccinia expressing interleukin-2 for cancer gene therapy.

Use of a recombinant vaccinia virus expressing human interleukin-2 (IL-2) was evaluated for preparation of tumor vaccines. A/J mice were immunized against neuroblastoma (C1300) cells using a preparation of C1300 cells infected/transfected with the recombinant virus, vCF13, expressing IL-2. A second recombinant vaccinia, vSC8, expressing Escherichia coli beta-galactosidase, was used as a control. After three weekly immunizations with virus-transfected cells, the mice were challenged with 1 x 10(6) unmodified C1300 cells and tumor development was monitored. Tumor development in the mice was inhibited by immunization with vCF13-transfected cells, compared to those vaccinated with vSC8-transfected cells (P < .008). A group of mice (7/15) immunized with vCF13-transfected cells followed by tumor challenge survived more than 60 days, at which time all mice immunized with the control vaccine were dead (p < .006). Five of the mice treated with the vCF13 vaccine were alive for more than 75 days (P < .05), after which they were rechallenged with another dose of 1 x 10(6) unmodified tumor cells. Tumor development was not apparent in these mice for more than 45 days following the second challenge, suggesting that these mice were completely protected by this immunization. These results demonstrate that recombinant vaccinia virus expressing IL-2 may be useful for cancer gene therapy.

Animals↗