An acute anaerobic infection following endodontic treatment.
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Biomedical subjects
Publications and source records attributed to H R Attebery.
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Feces from 25 subjects with colonic polyps (multiple adenomatous, large single, or single with atypia) and from 25 matched control subjects were studied by detailed quantitative aerobic and anaerobic techniques, using a large battery of culture media and several atmospheric conditions. Over 55% of organisms detected on microscopic count were recovered anaerobically. In several cases, there were significantly different numbers of organisms of specific types recovered from the two different populations studied. However, these differed from organisms with "statistical significance" noted in a previous study from this laboratory involving two different diet groups (Japanese Americans on either a Japanese or a Western diet). Specific differences in bacteriology between groups with different risks of bowel cancer, noted in earlier British studies, were not noted in our present or previous study.
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Fecal bacterial cultures from 40 normal humans yielded Megasphaera elsdenii from four individuals and Acidaminococcus fermentans from 10 individuals, with two individuals having both organisms.
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A total of 300 patients undergoing various types of urological procedures was studied for incidence of bacteremia. An osmotically stabilized anaerobic broth with sodium polyanethol sulfonate (Liquoid) yielded more positive blood cultures than any other culture system and was also the best system by far for recovery of anaerobes. The membrane filter showed faster growth and, therefore, facilitated faster identification of the infecting organism. There was a 31% incidence of bacteremia in the patients having transurethral resection of the prostate, 17% in the cystoscopy group, 24% in the urethral dilation group, and 8% in the urethral catheterization group. The organisms found most frequently isolated in blood cultures were enterococci and Klebsiella pneumoniae. Notable were a relatively large number of anaerobes and two protoplasts. The major source of the bacteremia was previous urinary tract infection, but evidence is presented which indicates that the prostate gland and the normal urethral flora are other significant sources.
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A double-closured method introduced for Hungate tubes eliminates clamps or special racks to hold the rubber stoppers in place while the tubes are being processed. The system prevents stoppers from being dislodged by gas-producing anaerobes and keeps the stoppers sterile so that the closed system of transfer can be conveniently utilized. Open and closed systems for handling a gas-producing anaerobe were compared. Ten tubes containing Clostridium perfringens were opened; the organism was detected in the air by use of an impingement collector. The gloved hand of the operator also revealed the test organism. A similar trial with culture material removed by the closed system (syringe transfer) resulted in no detectable contamination of the environment.
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