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Biomedical subjects

H R Buckley

Publications and source records attributed to H R Buckley.

11 recordsLinked to original sources
PubMed1979

Characterization of antigens from Aspergillus fumigatus. IV. Evaluation of commercial and experimental preparations and fractions in the detection of antibody in aspergillosis.

Commercially available preparations of Aspergillus fumigatus, experimental extracts and fractions of A. fumigatus, and extracts of 5 other aspergilli were evaluated in a blind manner for their abilities to detect antibody in 66 serum specimens from patients with aspergilloma, allergic bronchopulmonary and invasive aspergillosis, and unconfirmed aspergillosis. Rocket immunoelectrophoresis was more sensitive than immunodiffusion in 2 dimensions. Although qualitative differences in antigen composition could be detected, commercial preparations compared favorably with the best experimental extracts in detecting positive specimens. Extracts from young, actively-growing myceliums were most effective and produced the largest numbers of precipitin bands. In contrast to all unfractionated preparations, several fractions of A. fumigatus were devoid of substances that react with C-reactive protein of serums, yet were as effective as the best preparations in detecting serums positive for Aspergillus.

Antibodies, Fungal↗
PubMed1978

Increasing the predictive value positive of the precipitin test for the diagnosis of deep-seated candidiasis.

Three hundred fifty human sera were tested by double immunodiffusion, crossed-line electrophoresis, and crossed immuno-affinoelectrophoresis with a concanavalin A intermediate gel for precipitating antibodies to antigens present in cytoplasmic extracts of Candida albicans. Sera from 48 of 287 hospitalized patients at risk of invasive candidiasis contained precipitating antibodies to Candida antigens. Of these 48 sera, 27 had precipitating antibodies only to cell-wall antigens present in the cytoplasmic extract, and 21 sera had precipitating antibodies to both cytoplasmic and cell-wall antigens. The latter sera came from patients who were 2.5 times as likely to have deep-seated candidiasis as those patients with precipitins exclusively to cell-wall antigens. Sera from seven of 22 patients with vaginal candidiasis and 10 of 41 patients with other fungal infections had precipitating antibodies to C. albicans cell-wall antigens; only two of these sera also contained precipitating antibodies to the cytoplasmic antigens. Crossed immunoaffinoelectrophoresis with concanavalin A reduced the number of false-positive results and increased the predictive value positive of the precipitin test for deep-seated candidiasis from 31% to 71%.

Antigens, Fungal↗
PubMed1977

Cell wall antigens in soluble cytoplasmic extracts of candida albicans as demonstrated by crossed immuno-affinoelectrophoresis with concanavalin A.

Crossed immuno-affinoelectrophoresis with Concanavalin A precipitated four antigens from soluble extracts of the yeast and mycelial phases of Candida albicans. Two of these antigens were also present in spheroplast preparations. One of the Concanavalin A precipitated antigens was destroyed by periodate and none were digested by pronase. Sequential treatment with pronase and periodate destroyed two of the remaining three antigens. The Concanavalin A precipitated antigens appear to be glycoproteins, two of which are associated with the cell wall of C. albicans.

Antigens, Fungal↗
PubMed1977

Characterization of antigens from type A and B yeast cells of Histoplasma capsulatum.

The antigenic composition of cytoplasmic extract and culture filtrate antigens of type A and B yeast cells of Histoplasma capsulatum grown in a synthetic medium was studied. These preparations from type A and B yeast cells contained varying amounts of protein, carbohydrates, and protein-carbohydrate complexes. The antigenic analysis of these preparations was performed by antigen-antiserum absorption with subsequent immunodiffusion and cross-immunoelectrophoresis with absorption in situ in an intermediate gel. All protein antigens observed in culture filtrate of either type A or B yeast cells were also present in the cytoplasmic extracts of the same type. The cytoplasmic extract of type A and B yeast cells each contained certain characteristic antigens that were not shared by the other type. Rocket immunoelectrophoresis with different molecular weight fractions of the antigenic preparations from both types indicated the polydisperse nature of Histoplasma yeast cell antigens.

Antigen-Antibody Reactions↗
PubMed1975

An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida Albicans.

A chemically defined medium composed of 6 amino acids, biotin, inorganic salts and glucose for the growth of yeast and mycelial phases of Candida albicans at 25 degrees C and 37 degrees of C respectively was developed based on the aminopeptidase(s) profile of the fungus. This medium has proved successful in maintaining the growth characteristics of both phases during serial transfers. The relative pathogenicity, virulence, invasiveness and immunogenicity of the yeast and mycelial phases are discussed.

Amino Acids↗
PubMed1975

Cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans.

Crossed immunoelectrophoresis with absorption in situ was used to distinguish the cytoplasmic antigens unique to the mycelial or yeast phase of Candida albicans from cytoplasmic antigens shared by both phases. The soluble cytoplasmic extracts of each growth phase had at least six distinct antigenic constituents not shared by the other phase. This technique is recommended for the analysis of closely related antigenic complexes.

Animals↗