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Biomedical subjects

H R Gilbert

Publications and source records attributed to H R Gilbert.

At least 19 recordsLinked to original sources

Vocal fundamental frequency characteristics of infant hunger cries: birth to 12 months.

The vocal fundamental frequency (Fo) characteristics of four infants' hunger cries are reported. The infants were audio recorded at monthly intervals during the first year of life. In general, each infant's Fo increased across the one-year period. The increase in Fo was attributed to the transition from early reflexive crying to intentional vocalization behavior, rather than anatomical and physiological growth influences. Discussion focuses on the regulation of infant crying behavior across the first year of life, as well as the diagnostic implications of these findings.

Aging

A continuous-flow model for phonatory reaction time.

The purpose of this study was to validate a proposed continuous-flow model of phonatory reaction time by investigating the temporal order of selected laryngeal and neurophysiological events involved in a phonatory reaction time task. Ten normal speakers participated in a phonatory reaction time task. Laryngeal positioning movements prior to vocal fold closure (laryngeal shift) and onset of vocal fold vibration (acoustic onset) were recorded with an electroglottograph. P300 brain potentials were collected simultaneously, and they served as an index of a central process underlying reaction time. The obtained temporal ordering of laryngeal shift, P300, and acoustic onset supported a continuous-flow model of phonatory reaction time. Use of this model might yield information that is more accurate in explaining physiological function and more precise in describing temporal patterning than the serial model.

Adult

Nasal airflows in parkinsonian speakers.

Velopharyngeal control in individuals with Parkinson's disease (PD) was studied by obtaining rates of nasal airflow during syllable repetitions. Nasal airflows were described with respect to disease progression, phonetic context, and speaking rate. Significant differences in nasal airflows were obtained for disease progression and phonetic context. The data suggested that velopharyngeal control deteriorates systematically with disease progression. Parkinson's patients at mild and moderate levels of disease progression differentiated the nasal consonant from the oral consonants with increased nasal airflow associated with the nasal consonant. Differences, while not significant, were also observed in nasal flow rate values when the PD patients increased speaking rate from 1 to 5 syllables per second.

Aged

A respirometric technique to evaluate velopharyngeal function in speakers with cleft palate, with and without prostheses.

There is a paucity of respirometric quotient (RQ) data on individuals with velopharyngeal inadequacy. Paesani (1964) reported data using a technique that involved separate productions of the same task to obtain the RQ. The RQ values obtained were greater than unity, which is theoretically impossible. In the present study, respirometric quotients, the ratio of oral air volume expended to total volume expended, were obtained using separate but simultaneous productions of oral and nasal airflow. RQ values were calculated for 10 speakers with cleft palate, with and without their prosthetic appliances, and 10 normal speakers. As a group, those with cleft palate and without their appliances exhibited RQ values that were significantly lower than values obtained from the normal speakers and from speakers with the appliances in place. These findings indicated that there were no statistically significant differences in RQ values when comparing sentence repetition and counting tasks. These values were lower than those obtained for the nonnasal syllable repetition tasks, with the /mv/ syllable repetition task generally being associated with the lowest RQ value of any of the speech tasks. The correlation between RQ values and perceptual judgments was -.60, indicating that there was modest agreement between the two measures. As RQ values decreased, perceptual judgments of nasality increased.

Adolescent

Relation between voice profile ratings and aerodynamic and acoustic parameters.

The present study sought to examine the relation between Wilson Voice Profile ratings and certain aerodynamic and acoustic variables. Subjects in this study were 20 adults with voice disorders and five adults with no voice disorders. A series of stepwise multiple correlations were used. The variance in perceptual ratings accounted for by aerodynamic and acoustic variables included in the present study ranged from 29% for tension ratings of connected speech to 81% for pitch ratings of connected speech for the female speakers. It is clear from the present findings that ratings on the Wilson Voice Profile are only partially accounted for by the variables included in the present study.

Adolescent

Laryngograph as a measure of vocal fold contact area.

The present investigation sought to provide additional information concerning the laryngograph as a means to study vocal fold contact area. Subglottal pressures were sensed simultaneously with the laryngographic signal while the speaker produced a variety of speech tasks. The onset and cessation of the subglottal pressure waveform was studied relative to the laryngographic and speech waveforms. Differences were noted for voiced-voiceless contrasts for bilabial stop consonant production and vocal effort changes during the three vowels studied. Also a high-resistance polymer strip was placed between the vocal folds and gradually removed while simultaneous laryngographic recordings were obtained during sustained productions of the vowel /a/. An increase in the amplitude of the laryngographic waveform upon withdrawal of the polymer strip strongly supported the concept that the laryngographic signal is generated directly by the change in conductance due to alterations in the area of vocal fold contact.

Adult

Preparation, purification and properties of a crosslinked trimer of G-actin.

Phenylenebismaleimide has been used to form crosslinks between actin monomers [Knight, P. and Offer, G. (1978) Biochem. J. 175, 1023-1032]. We have purified a trimer of actin monomers as well as a dimer and a mixture of higher molecular weight oligomers. The trimer is much more effective than the dimer in enhancing the rate of polymerization while higher oligomers do not appear to be any more effective than the trimer. A lag in the polymerization process, as measured fluorescence enhancement of trace pyrene-actin, still occurs in the presence of trimers serving as the nuclei, suggesting that the mechanism for polymerization is more complex than nucleation followed by elongation.

Actins

Relation of selected acoustical parameters and perceptual ratings to voice quality of Down syndrome children.

Judges using the Wilson Voice Profile rated the voice quality of 14 noninstitutionalized, 7- to 10-year-old Down syndrome children and a group of nonretarded children. The Down syndrome children received ratings different from the nonretarded children on the Severity subscale of the Wilson Voice Profile. Voice samples were analyzed acoustically in terms of fundamental frequency, fundamental frequency range, frequency and amplitude perturbations, and spectral noise-to-harmonic component ratios. Frequency perturbations, amplitude perturbations, and spectral noise-to-harmonic component ratios were significantly greater for the Down syndrome children. There were also significant correlations between judges' Severity ratings and frequency perturbation levels, amplitude perturbations levels, and spectral noise-to-harmonic component ratios.

Child

A respirometric technique for evaluating velopharyngeal closure in children.

Respirometric quotients, indices of velopharyngeal closure, were determined from oral and nasal air volumes measured separately but simultaneously. Ten children aged 6-10 years were the subjects. The quotients were significantly lower for speech stimuli involving nasal sounds than for those involving nonnasal sounds. In contrast to the quotients obtained in previous research with children, the quotients obtained in this study were close to, but not in excess of, unity.

Child

A fluorescent probe for conformational changes in skeletal muscle G-actin.

Actin from rabbit skeletal muscle has been modified with the fluorescent label N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylenediamine (1,5-I-AEDANS). Under conditions where the actin is in the unpolymerized form (G-actin), the addition of Mg2+ or KCl results in enhancement of the fluorescence. Titration of the labeled G-actin with Mg2+ at varying concentrations of CaCl2 gives, by extrapolation, a value for the dissociation constant for Mg2+ of 35 microM in the absence of Ca2+ and a calculated value of 10 microM for Ca2+ in the absence of Mg2+. The two metal ions compete with each other. The fluorescence enhancement induced by Mg2+ is reversed by the addition of Ca2+ and both processes are time-dependent, indicating a reversible conformational change of G-actin as a consequence of addition of divalent metal. KCl also enhances the fluorescence of the labeled G-actin but does not appear to compete with the divalent metal ion. The enhancement of the fluorescence is very rapid and any conformational change induced by KCl is probably different from that induced by divalent metal ions. Finally, it is shown that loss of fluorescence of the labeled G-actin may be associated with inactivation of the actin.

Actins

Speaking fundamental frequency in three groups of hearing-impaired individuals.

The purpose of the present investigation was to provide speaking fundamental frequency data for three groups: 4-6, 8-10, and 16-25-yr-old hearing-impaired individuals. The hearing-impaired children between the ages of 4 to 6 and 8 to 10 yr exhibited mean speaking fundamental frequency values that were 55 Hz and 41 Hz higher, respectively, than values obtained for normally hearing children of similar ages. Fundamental frequency values for postpubescent hearing-impaired males and females were 20 Hz and 30 Hz higher, respectively, when compared to values reported in the literature for normally hearing individuals of similar age and sex.

Adolescent

Spectral properties of fricative consonants in children.

Twenty-one preadolescent children were asked to produce stimulus words with the fricatives /f/, /v/, /s/, /z/, /s/, and /3/ in various phonetic environments. Results indicated that the major resonances were higher than values reported for both male and female adult speakers. Also significant differences existed among the major resonances of the consonants studied.

Child

Malate dehydrogenases. The lack of evidence for dissociation of the dimeric enzyme in kinetic analyses.

The kinetic parameters of beef heart cytoplasmic and pig heart mitochondrial malate dehydrogenases have been examined over a wide range of enzyme concentration. No significant changes are observed in these properties. In conjunction with active enzyme sedimentation and sedimentation equilibrium experiments, it is concluded that there is no evidence for dissociation of the dimeric enzyme at any enyzme level in the kinetic analyses. Thus, if dissociation occurs, it must be too slow to be of significance in determining the kinetic properties of the enzyme. It is shown that unless a subunit and its dimeric form have identical kinetic and substrate binding characteristics, the kinetic parameters should change as a function of enzyme concentration.

Animals