The nodulin 24 protein family shows similarity to a family of glycine-rich plant proteins.
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Amino Acid Sequence↗
Biomedical subjects
H Richter
Publications and source records attributed to H Richter.
Financing health services in Sub-Saharan Africa: options for decision makers during adjustment.
The financing of health services has become an increasingly critical and urgent issue in many developing countries particularly in Sub-Saharan Africa. This paper analyses options available to policy makers. The possible effects and side effects of strategies are described based on the experience from different countries. The dangers of simplistic solutions are discussed. A cautious approach is recommended taking into consideration the lessons learned in other regions accompanied by a careful ongoing evaluation especially regarding the ability to pay of the poorer sections of the population. Providing for equity in health care should be an important guiding principle. It therefore appears to be necessary to find an appropriate mix of public and private sector interventions with elements of cost-sharing for services and drugs, insurance schemes and more efficient use of available resources.
Africa↗
Maintenance of left ventricular function (90%) after twenty-four-hour heart preservation with deferoxamine.
During 24-h in vitro heart preservation and reperfusion, irreversible tissue damage occurs caused by reactive oxygen intermediates, such as superoxide radicals, singlet oxygen, hydrogen peroxide, hydroperoxyl, hydroxyl radicals, as well as the peroxynitrite radical. Reduction of the related oxidative damage of reperfused ischemic tissue by free radical scavengers and metal chelators is of primary importance in maintaining heart function. We assessed whether deferoxamine (DFR) added to a cardioplegia solution decreased free radical formation during 24-h cold (5 degrees C) heart preservation and normothermic reperfusion (37 degrees C) in the Langendorff isolated perfused rat heart. The deferoxamine treated hearts were significantly (p less than .001) better preserved than the control hearts after 24 h of preservation with regard to recovery of left ventricular diastolic pressure, contractility (+dP/dt), relaxation (-dP/dt), creatine kinase release, and lipid peroxidation. DFR preserved cell membrane integrity and maintained 93% of left ventricular contractility. The evidence suggests that DFR reduces lipid peroxidation damage by reducing free radical formation and thereby maintaining normal coronary perfusion flow and myocardial function.
Animals↗
Postprandial hyperlipemia does not inhibit platelet aggregation.
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Adult↗
[Risk factors of atherosclerosis--primary examination of Vienna employees].
In the course of an atherosclerosis intervention study, a basic medical check-up of 2105 out of 4800 employees of a Viennese banking house was carried out (37.1 +/- 11.0 years, 54.6% females). Apart from liver and kidney function parameters, an extensive lipid status was determined, the blood pressure was measured, and a cardiovascular-centered case history ascertained. The mean cholesterol level in females was 208.3 +/- 54 mg/dl and that in males was 226.8 +/- 61.1 mg/dl. The HDL-cholesterol level in males was 43.8 +/- 11.9 mg/dl; that in females (54.9 +/- 13.4 mg/dl) was significantly higher. The mean value of LDL-cholesterol in the entire group was 141.6 +/- 51.4 mg/dl and it was significantly higher in males, as well as in participants with known hypertension. 33.4% of the persons stated that they smoked. The obtained data will serve not only to discover potential interrelations between the individual risk factors of atherosclerosis in Austria, but also in particular as a basis for interventional strategies on the part of the company's medical services.
Adolescent↗
Immobilization of soluble fibrin on factor XIIIa-coated polystyrene beads mediated by N-terminal fibronectin fragments. II. Demonstration of covalent adducts of fibrin peptide chains and fibronectin fragments.
Previous experiments had shown that the free N-terminal fibronectin 30-kDa-domain mediates binding of soluble 125I-fibrin to transamidase-coated polystyrene beads (Hörmann et al., Biol. Chem. Hoppe-Seyler 368, 669-674, 1987). Now, the formation of covalent adducts of the N-terminal fragment with fibrin peptide chains is demonstrated. Binding of soluble 125I-fibrin was performed in presence of N-terminal fibronectin 30-kDa or 70-kDa fragments. The material adsorbed was removed from the beads under reducing conditions and analysed by dodecylsulfate gel electrophoresis followed by autoradiography. The 30-kDa fragment gave rise to bands of 80 kDa and 180-200 kDa which were lacking in the products of the 70-kDa compound. Instead, they showed bands at 120 kDa and ca. 280 kDa. Evidently, those bands represented covalent adducts of fibrin peptide chains or their dimers with the 30-kDa or the 70-kDa fragment, respectively. In addition, dimeric gamma-chains and alpha-chain polymers of fibrin were present indicating partial polymerization of bead-attached fibrin.
Electrophoresis, Polyacrylamide Gel↗
[Mobilization following deep venous thrombosis of the leg].
A simple score for mobilisation of patients with deep venous thrombosis of the leg is proposed. By this score the significantly earlier mobilisation of patients with deep venous thrombosis is possible without risk.
Double-Blind Method↗
[Studies on the cell and tissue tolerance of a polymeric bone replacement material].
BOP (Biocompatible Osteoconductive Polymer) shows no cytotoxicity in vitro. After six months of subcutaneous implantation in mice a weak cellular tissue reaction is seen histologically. The hard tissue reaction after six months of implantation in fractured tibia bone shows less connective tissue between the implant material BOP and bone.
Animals↗
[Congenital clavicular pseudarthrosis].
Three children with congenital clavicular pseudarthrosis are reported in this paper. Reference is made to indications for surgery and to the surgical approach.
Child↗
[A new method of transosseous pull-out wire fixation in ligamentous injuries of the metacarpophalangeal joint of the thumb].
Surgical repair is usually indicated for rupture of the ulnar collateral ligament of the MP-joint of the thumb. The pull-out wire technique is commonly used for repair of typically distal ligamentous ruptures or avulsion fractures at the base of the proximal phalanx. The method's disadvantages include skin or soft tissue necrosis beneath the button on the radial side of the thumb. When postoperative swelling subsides, the formerly taught wire loosens and so does the repair. Because of this inherent danger, a special steel sleeve which is drawn over the wire on the radial side of the thumb until bone contact is made was developed. Thus, a strong and unyielding fixation of the ulnar collateral ligament repair or avulsed bone chip is certain. The follow-up for 41 patients treated by this technical modification is presented; there were no complications, including wound infections.
Bone Wires↗
[Psychological studies of families with children and adolescents following surgery for congenital heart defects (tetralogy of Fallot and aortic coarctation)].
Sixty-two children with operated congenital heart disease (34 with tetralogy of Fallot and 28 with coarctation) ranging in age from 9 to 16 years underwent a detailed psychological examination. The results were compared with children without structural heart disease and with test norm values. The aim of the study was to check findings on intelligence and explore aspects which had been hardly considered previously (for instance learning capability, concentration, mental work-up of disease etc.). We had assumed that there were differences between the Fallot group and that of coarctation, since the former had been handicapped physically prior to surgery. There were differences in terms of logical thinking, where children with coarctation turned out to be better. As far as other variables were concerned there was no significant difference (such as behavioural features), nor was there any difference between boys and girls. The results show that psychic "problems" of cardiac children do not resolve automatically after surgery, but there may be children with persisting psychological peculiarities who require guidance, occasionally even psychotherapy.
Adolescent↗
Cell-binding and internalization of soluble fibrin by platelets.
Centrifuged human platelets bound soluble 125I-labelled fibrin, mediated by a plasma factor. Binding was inhibited by D-phenylalanyl-L-prolyl-L-arginyl- chloromethane (PPACK), which specifically blocks thrombin. As the binding-promoting principle was adsorbed to barium citrate, it was tentatively characterized as prothrombin, suggesting that it might be converted to thrombin at the cell surface. The peptide GRGDSP failed to inhibit binding, thus eliminating the glycoprotein IIb/IIIa complex as a receptor. Most likely, a thrombin - fibrin complex is recognized by a cell receptor, possibly protease-nexin I. In a platelet concentrate, the cells also internalized 125I-labelled fibrin, providing evidence that platelets are involved in the clearance of circulating fibrin - monomer complexes. Engulfment was again inhibited by PPACK or hirudin but not by an antibody against the glycoprotein IIb/IIIa complex.
Amino Acid Chloromethyl Ketones↗
Plasma free N-terminal fibronectin 30-kDa domain as a marker of endothelial dysfunction in type 1 diabetes mellitus.
The plasma free N-terminal fibronectin 30-kDa domain was measured in 44 type 1 diabetic patients and in 20 healthy subjects. A significantly raised mean concentration of a free N-terminal fibronectin 30-kDa domain was found in plasma of diabetic patients with proliferative retinopathy as compared with healthy persons (P less than 0.001). A positive correlation was observed between free N-terminal fibronectin 30-kDa domain and von Willebrand factor in plasma of all examined subjects (r = 0.62, P less than 0.01). A similar correlation was present between 30-kDa domain and albuminuria (r = 0.56, P less than 0.01). However, no relationship was found between fibronectin 30-kDa domain and control of diabetes as assessed by fructosamine concentration. The free N-terminal fibronectin 30-kDa domain may be used as a marker of actual endothelial cell dysfunction in diabetes.
Adult↗
Chicken gizzard 5'-nucleotidase is a receptor for the extracellular matrix component fibronectin.
The smooth muscle cells of chicken gizzard harbor the ectoenzyme 5'-nucleotidase. The purified enzyme was reconstituted into 3H-labeled proteoliposomes which were used as a model to study the association of a membrane protein with fibronectin. We demonstrated that the binding process between proteoliposomes and fibronectin has the qualities of a receptor-ligand interaction, i.e., is saturable and specific. In contrast to the association of fibronectin with integrins, the interaction with 5'-nucleotidase does not require divalent metal ions. Synthetic peptides containing the RGD-sequence or a monoclonal antibody interfering with binding of other receptors to the cell-binding domain of fibronectin did not abolish the interaction with 5'-nucleotidase. This indicates that the RGDS-sequence does not represent the major contact site for the AMPase and that the 5'-nucleotidase belongs to a separate class of fibronectin receptors with distinct properties as compared to the integrins.
5'-Nucleotidase↗
[The effect of drugs on laboratory diagnosis. The effect of ascorbic acid on selected automatic laboratory methods].
The paper presents further investigations for a critical survey on the influences of drugs on laboratory methods. In controversion to the meanings you can find in the literature that ascorbic acid is most one of the important drugs to interfere with laboratory results we couldn't see in our systematical experimental investigation such results. Only in very extremly cases it seems to be right. Selected parameters of clinical chemistry (glucose, lactic dehydrogenase, aspartal-aminotransferase, alanine aminotransferase, alkaline phosphatase, protein, albumine, creatinine, butanol extractable iodine, ferrum) show under therapeutic conditions no influences of ascorbic acid, which can lead to diagnostic or therapeutic false interpretations. Above all the often mentioned example that glucose estimations in blood (reduction methods) can disturb if ascorbic acid is present, is abstracted in an uncritical manner how our experimental results may show.
Ascorbic Acid↗
[Red cell metabolism and function in shock (author's transl)].
In patients with shock the metabolism and O2-function of the erythrocytes were studied following separation according to cell-age by density layer centrifugation using a new supporting medium. Metabolic acidosis in patients with postoperative shock (n=8) and in patients with shock due to severe congestive heart failure (n=12) provoked decreased levels of 2,3-diphosphoglycerate, ADP, ATP and reduced glutathione. Raised lactate/pyruvate ratios were observed also in both groups, whereas the O2-function of the erythrocytes was markedly disturbed in all patients. Patients of both groups received glucocorticoid-infusions resulting in a significant improvement of the extent and pattern of the red cell metabolic disorder.
Adult↗
[Late sequalae of ovarian torsion in the child].
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Age Factors↗
A scanning microinterferometer with correction of errors caused by overlapping 'ghost' images.
An experimental setup has been built to correct the errors caused by overlapping 'ghost' images when scanning and integrating measurements are carried out with the Jamin-Libedef interference microscope. First a reference field with the object, producing the 'ghost' image, is scanned and the values of the optical path difference (OPD) of each point in the reference field are stored in a computer. Subsequently, the OPD of the object to be measured is calculated for each point by adding the measuring result to the average OPD of five points, located around the corresponding point in the reference field. The applicability of the setup has been tested by measurements with Sepharose beads, Hela cells and the Zeiss microinterference refractometer. The difference between measurements on the same object with and without overlapping 'ghost' image was about 10%. The reproducibility was tested by repeated measurements on the same cell whereby a standard deviation of 1.1% was found.
Computers↗