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Biomedical subjects

H Ripoll

Publications and source records attributed to H Ripoll.

15 recordsLinked to original sources

Age-related differences in the preparatory processes of motor programming.

This article investigates the mechanisms underlying the age-related differences in information processing in the production of motor responses, especially the development of feedforward mechanisms. No age-related differences have emerged from developmental studies aiming at analyzing motor programming. Nevertheless, age effects have seldom been studied in function of motor preparation. The aim of the present experiment was (1) to study age differences in motor preparation, and (2) to validate the early maturation of movement parameters specification. Two conditions were used (1) no advanced information on the movement to be made was given to the subject (neutral prime), and (2) advanced information on which direction should be followed was provided to the subject, allowing him to prepare a response based on a prime (primed condition). Four age groups were studied 6, 8, 10 and 22 years. Our results showed mainly an early maturation of the programming processes. More specifically, (1) beyond 6 years of age, children are capable of using the information provided by the prime to prepare their movement in advance, (2) costs and benefits of pre-programming do not vary significantly with age, (3) deprogramming-reprogramming of effector and direction is quite similar across the four age groups.

Adult

Effects of tennis practice on the coincidence timing accuracy of adults and children.

This study examines the development of perceptuomotor processes involved in coincidence timing tasks according to age and experience in tennis. Tennis players and novices, 7, 10, 13, and 23 years of age, were tested in a coincidence timing task which consisted of estimating the arrival of a simulated moving object on a target. The effect of three different motions were analyzed: constant velocity, constant acceleration, and constant deceleration. Results showed that (1) timing accuracy improves mainly between the ages of 7 and 10 years; (2) tennis practice accelerates the development of timing accuracy; and (3) acceleration or deceleration of the moving stimulus had no effect on the timing accuracy of any of the tested groups, suggesting a continuous visual control of the trajectory. Theoretical implications for the development of perceptuomotor processes involved in coincidence timing tasks are discussed.

Acceleration

Effect of expertise on coincident-timing accuracy in a fast ball game.

The aim of this study was to examine the effect of intensive practice in table tennis on perceptual coincident timing. The main question was whether the perceptual demands encountered in fast ball sports produce modifications of the perceptual visual system. Expert table tennis players and novices were compared in a perceptual task which consisted of estimating, by pressing a key, the arrival of a moving stimulus at a target. The stimulus, which was presented either at constant velocity or at constant deceleration, reproduced as closely as possible the natural visual demands encountered in table tennis. The difference between the time of response and the time of arrival of the stimulus at a target position was measured over 40 trials for each of the 16 participants. The results showed no effect of expertise under the constant-velocity condition but an effect under the decelerative condition, indicating that experts were less trajectory-dependent than novices. This result was interpreted as reflecting a better adaptation of the perceptual system of experts to the constraints encountered during table tennis and specifically to the perceptual demands resulting from varied and decelerated ball trajectories. Finally, some limitations of the coincidence anticipation procedure are highlighted, concerning its use in practical settings for evaluating athletes or detecting sport talents, and the need for the simulation conditions during testing to reproduce as closely as possible the perceptual demands of real life is discussed.

Adaptation, Physiological

Age differences in using precued information to preprogram interception of a ball.

The present experiment examined the development of programming interception of a ball's movement across three groups of children ages 6, 8, and 10 years, who were compared with adults. In an interception task we manipulated the subjects' preparation by using Rosenbaum's 1980 precuing procedure. Two levels of precued information were used concerning the effector specified (right or left arm) and the direction of the arm projection (outside or inside). We focused particularly on RT and response errors. Analysis indicated RT decreased across the age groups and errors decreased mainly in the nonprecued condition. A critical period in improvement might be at age 8 as children use precue information to program their movements as efficiently as adults. The duration of effector programming was similar for children and adults but was more precise for the older subjects. For children as well as for adults, the specification of direction occurs after movement initiation and not before.

Adult

Covert orienting to central visual cues and sport practice relations in the development of visual attention.

This study examines the development of controlled covert orienting of visual attention, according to the age and the level of performance of tennis players. Practicers and nonpracticers age 13, 16, and 25 participated in a covert orienting task. On each trial, subjects responded to a target which appeared in one of four locations arrayed horizontally across the display. Three central cues were used: neutral, the cue did not specify in which location the target would appear; valid, the target was present in the cued location on 80% of the trials; invalid, the target was present in one of the uncued locations on the remaining 20% of the trials. The results showed that (1) practicers were not faster than nonpracticers in processing the signals, (2) observers of all ages oriented attention voluntarily to the cued locations, (3) central cues had a smaller effect on older and practiced subjects, and (4) developmental and sport practice factors had similar effects on orienting efficiency. The implications of these findings for theories of attentional development are discussed.

Adolescent

Uncertainty and visual strategies in table tennis.

Ball games are characterized by perceptual uncertainty and time-pressure. Of interest is the visual-search pattern carried out when these characteristics change. This is the case in table-tennis situations when comparing a drill situation, when one kind of stroke is constantly repeated, and a match situation, when the stroke is more often unpredictable. We analysed, during play, the visual-search pattern of five expert table tennis players to examine the effect of uncertainty on visual behavior. Direction of gaze was recorded by a video-oculographic recorder (NAC Eye Mark Recorder IV). Analysis showed that (i) visual fixations towards opponent player were only systematic in a match contrary to drill where they occurred less frequently. (ii) Whatever the situation, only the first part of the ball's trajectory was visually tracked. This occurred immediately after the opponent's release of the ball. Nevertheless, visual tracking was more frequent and of longer duration in a match. (iii) Analysis of motor behavior showed that the duration of the movement preparation was longer in a match while the duration of the execution phase was unchanged.

Eye Movements

Malondialdehyde production from spermine by homogenates of normal and transformed cells.

The oxidation of spermine in vitro by a mixture of polyamine oxidase and diamine oxidase from pig kidney gives rise to malondialdehyde via 3-aminopropanol as the intermediate. Conversely, with spermidine, under similar experimental conditions, no evidence could be obtained for malondialdehyde formation within the limits of sensitivity of the assay (2.0 nmol). The activities of both these enzymes show about a 2-fold increase in normal rat kidney cells (LA31 NRK) transformed by the temperature sensitive mutant of Rous sarcoma virus (LA31) and incubated at the non permissive temperature (39 degrees C) compared to the activities in LA31 NRK at the permissive temperature (33 degrees C). These same enzymatic activities show no temperature dependent changes in normal rat kidney cells (NRK) or in these same cells infected by the wild type virus (NRK B77). In extracts derived from Friend erythroleukemic cells induced to differentiate by dimethyl sulfoxide or hexamethylene bis acetamide, spermine oxidation takes place more efficiently than in non induced cells. A rise in diamine oxidase activity is seen in LA31 NRK (39 degrees C) 12 h after the temperature shift, whereas morphological manifestations of normalcy are seen only at 48 h. The Km of diamine oxidase is 10(-6) M for putrescine and 10(-3) M for 3-aminopropanol. A possible mechanism involving the well documented acetylation of putrescine [23,26] is proposed for diverting intracellular putrescine away from cytosolic diamine oxidase and towards intramitochondrial monoamine oxidase.

Aldehydes

Analysis of visual scanning patterns of pistol shooters.

Visual behaviour of pistol shooters of various levels of experience was studied using a video-oculometric technique to record the direction of gaze. A spatio-temporal analysis of the fixation patterns of subjects (five international pistol shooters and five international pentathletes) found that patterns were related to the level of skill. The pentathletes, whose level was lower, positioned their gaze on the weapon sight and visually followed the movement of the weapon towards the target. The pistol shooters appeared to use negative feedback in bringing up their weapons, so reducing the angle formed by the weapon, target and gaze. They fixed their gaze directly on the target or between the target and the weapon. Observations showed a prolonged time of attack (means = 1.107 s vs 0.558 s; P less than 0.001) and a shorter stabilization time (means = 0.714 s vs 1.216 s; P less than 0.001) for the most expert shooters. The psycho-physiological mechanisms which were the basis of these patterns and the reasons why shooters chose them are discussed.

Humans

Differences in polyamine availability and insertion into fibronectins released from normal and transformed cells.

1. Fibronectin released from transformed rat kidney cells compared with that released from normal rat kidney cells shows a 50% increase in amino group availability. 2. No such changes were observed in thiol and carboxy group availability or in sialic acid content. 3. The increased amino group availability is not due to a greater polyamine content, which was about 0.04 pmol/mg of protein. 4. Transglutaminase mediated the insertion of spermidine into normal cell fibronectin with linear kinetics. With fibronectin from transformed cells (temperature-sensitive mutant or wild-type), kinetics typical of substrate inhibition were observed. 5. Immunochemical analysis with an anti-polyamine antiserum and an anti-(human fibronectin) antiserum showed that fibronectins from normal and transformed cells react differently. The significance of these results is discussed in the light of changes in the secondary structure between the two fibronectins.

Animals

Diamine oxidase and polyamine oxidase activities in normal and transformed cells.

1. The activity of diamine oxidase (EC 1.4.3.6) in normal rat kidney cells and in normal rat kidney cells transformed by avian sarcoma virus (B77 strain) growing in tissue culture varies with the stage of growth. There is an initial stimulation of activity by 24h after seeding, followed by a steep decline during exponential growth (48-72h). Enzyme activity decreases even further as the cells reach saturation density (confluence) after 4 days in culture when the activity in normal rat kidney cells is twice as high as that in transformed cells. 2. Differences of about the same order of magnitude are observed between transformed human cells HeLa, HEp2 (a human epithelioid carcinoma) and normal human fibroblasts, in chicken cells between normal myeloblasts and leukaemic myeloblasts, and in rats between biopsy material from normal mammary tissue and 9,10-dimethylbenz[a]anthracene-induced mammary tumours. 3. Polyamine oxidase activity also varies with the growth of transformed rat kidney cells, but shows no significant variation with the growth of normal rat kidney cells between 24 and 96h after seeding. The activity in cells at confluence is from 3- to 5-fold lower in the transformed than in the normal rat kidney cells. 4. A similar 5-10-fold decrease in activity has been found in 9,10-dimethylbenz[a]anthracene-induced mammary tumours in rats and in human oesophageal tumours. 5. Possible reasons for these observations and the contribution of these two enzymes to cellular putrescine concentrations are discussed.

Amine Oxidase (Copper-Containing)

In vivo antitumor activity of 4-amino 4-methyl 2-pentyne 1-al, an inhibitor of aldehyde dehydrogenase.

4-amino-4-methyl-2-pentyne-1-al (AMPAL), a new irreversible inhibitor of aldehyde dehydrogenase (ALDH) has been assayed for its in vitro and in vivo antitumor activity. In vitro, AMPAL inhibits the proliferation and the ALDH activity of L1210 and RBL5 cell lines. In vivo, AMPAL significantly increases the mean survival time of mice i.p. grafted with leukemia (L1210, P815, MBL2, EL4, RBL5 cell lines) or carcinoma cells (Krebs cell line), without haematopoetic toxicity. No carcinostatic effect was observed against the P388 leukemia and the 3LL Lewis lung carcinoma. A possible relationship between the ALDH isoenzyme activity of the tumor and its sensitivity to AMPAL is discussed in the light of previous reports concerning the role of aldehydes in cell growth control.

Aldehyde Dehydrogenase