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Biomedical subjects

H Rohr

Publications and source records attributed to H Rohr.

At least 19 recordsLinked to original sources
PubMed1985

Stereologic and fine-structural studies of prostatic acinar basal cells in the dog.

There are two distinct types of epithelial cells in the lining of the glandular acini of the prostate in adult male Beagle dogs, i.e., the columnar secretory epithelial cells and the basal cells. In contrast to the secretory epithelial cells, basal cells exhibit an abundance of micropinocytotic vesicles on their basal surface. Blood capillaries are often found in the stromal tissue in close proximity to these cells and their walls frequently display chains of fenestrations bridged by diaphragms. Stereological analysis shows that the volume density of the basal cells in the reference volume of acinar parenchyma is 0.056, and there are approximately 132.14 million cells per cm3 of prostatic tissue. An average basal cell has a volume of 373.5 micron3, and the volume densities of its nucleus, rough endoplasmic reticulum, Golgi apparatus, mitochondria and micropinocytotic vesicles, are 0.49, 0.04, 0.04, 0.094 and 0.013, respectively. These data are distinctly different from those that have been reported for the prostatic secretory epithelial cells of the same animals.

Animals↗
PubMed1983

In vivo complement activation by polyanion--polycation complexes: evidence that C5a is generated intravascularly during heparin--protamine interaction.

In vitro studies have established the complement-activating potency of polyanion-polycation interaction. By transferring the heparin-protamine model to an animal (rabbit) system that picks up ongoing intravascular complement activation by documenting acute granulocytopenia due to margination, evidence that intravascular polyanion-polycation complexing leads to instantaneous activation of the C system in vivo that goes beyond C1, C4, and C2, and results in generation of biologically highly reactive products, such as C5a, is provided. Dependence of the phenomenon on an activable complement system is documented by its complete abolishment when animals were complement depleted by cobra venom factor administration. Therefore, activation of the complement system may play a causative role for untoward effects following heparin neutralization by protamine under clinical conditions. Furthermore, these studies suggest an in vivo role of nonimmune generation of inflammatory mediators by interaction of naturally occurring polyions.

Agranulocytosis↗
PubMed1982

Sonometrics of the prostate.

Determination of prostatic size by rectal examination is inaccurate if objective and reproducible values are desired. Sonometrics, a combination of transrectal sonography and biometrics, is a simple method to determine prostatic weight. The optomanual image analyzing system (BIT PAD-TRS 80) consists of a digitizer tablet, cursor, processing unit and keyboard (TRS 80). The photographic records or the image of the prostate on the scan can be measured with the optomanual image analysis system by tracing the circumference of the prostate. Using the computer one can calculate length, area and circumference of the sonogram. When the postoperative and sonometrically estimated weights were compared a good correlation coefficient was found. The studies on age-dependent growth of the prostate show an age-volume relationship similar to that shown in the study by Swyer. As demonstrated in patients with hypothalamic hypogonadotropic hypogonadism change in prostatic volume also can be assessed in small prostates.

Aged↗
PubMed1982

Spermiometrics: objective and reproducible methods for evaluating sperm morphology.

An approach was made to replace the subjective evaluation of human sperm morphology by an objective method applicable in routine laboratories. 20 patients out of infertile marriages (G2 and G3) were compared with 10 normal, fertile men (G1). Two different methods were used. Highly developed instruments and techniques were used in method 1, and cheap and simple ones, designed for routine laboratories, in method 2. Head area, circumference, length, and maximal midpiece width of 100 spermatozoa/subject were determined by a semi-automatic image analysis system. Head variation and average midpiece width were clearly bigger in G2 and G3 than in G1. As a result, formulae were developed, determining the pathological alteration of sperm head and midpiece for the individual semen. In addition, an objective classification into different sperm types was elaborated. Using the developed formulae, results of methods 1 and 2 are well in agreement. Thus, method 2 is recommended for routine laboratories, allowing an objective and reproducible morphology determination within 30 min.

Clinical Laboratory Techniques↗
PubMed1979

Quantification of human sperm morphology and motility by means of semi-automatic image analysis systems.

Sperm morphology and motility are determined using a semi-automatic image analysis system under opto-manual control. The system consists of a microscope equipped with a drawing-tube, a digitizer-tablet, and a cursor interfaced to a small microcomputer. A light-emitting diode is mounted on the cursor, visible as a bright red pilot-light in the microscopic field. Sperm head and midpiece as well as the pathway of motile spermatozoa are traced with the cursor's red pilot light. The microcomputer calculates area, circumference, and length simultaneously. Motility and sperm density are determined altogether using microchamber technique. In a selected group of male subjects with normal, doubtful, and pathological semen analyses biometrical analyses of sperm morphology and motility were performed. Sperm morphology is best described by the variation of the head size and by the average midpiece width. Motility is best described by % motile spermatozoa and mean velocity determined 1 hour after semen collection. Biometrical semen analyses are superior to subjective evaluations regarding degree of information, objectivity, and reproducibility.

Cell Survival↗