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H Rothe

Publications and source records attributed to H Rothe.

54 records · Page 3Linked to original sources
PubMed1991

The penile plication procedure: an alternative method for straightening penile deviation.

The number of reported cases of congenital and acquired penile deviation is evidently increasing. We describe our experience with the penile plication procedure, which we used to treat 40 patients with penile deviation. Postoperatively, all patients regained the ability for sexual intercourse, with potency remaining unimpaired. Of the patients 96% were completely satisfied with the result of the operation. Sustained complications have not been observed. To avoid major trauma the surgical intervention should not include removal of ellipsoids from the penile tunica albuginea as proposed by Nesbit. Since the penile plication method produces equally good results in terms of performance and appearance, this therapy should be preferred.

Adolescent↗
PubMed1991

Decreased expression of adhesion molecules on monocytes in recent onset IDDM.

The expression of adhesion molecules in monocytes of patients with recent onset type I diabetes was analysed. Monocytes were identified as CD14-positive cells by flow cytometry. The percentage of monocytes expression LFA-1 alpha, ICAM-1 and HLA-DR was slightly lower in recent onset type I diabetes (n = 13) compared to normal subjects (n = 15) and was significantly decreased after activation of cells with lipopolysaccharide and interferon-gamma for 5-24 hr. Receptor densities on adhesion molecule-positive monocytes and the expression of LFA-1 beta were normal. These data indicate that monocyte trafficking is abnormal in recent onset type 1 diabetes.

Adolescent↗
PubMed1990

Tumour necrosis factor alpha production is upregulated in diabetes prone BB rats.

Following activation peritoneal macrophages from diabetes prone BB rats secreted strikingly higher amounts of tumour necrosis factor alpha than found for macrophages from diabetes resistant or normal Wistar rats. Enhanced tumour necrosis factor alpha production was detected prior to the occurrence of insulitis. Cultures of macrophages derived from precursor cells in diabetes prone BB rat bone marrow also showed upregulated tumour necrosis factor alpha secretion upon challenge with endotoxin and interferon gamma. Tumour necrosis factor alpha hypersecretion may contribute to autoimmune diabetes by affecting thymic and post-thymic T-cell maturation and by promoting pancreatic islet inflammation.

Animals↗
PubMed1990

Biological properties and partial purification of a growth factor from porcine spleen.

3T3-Fibroblast growth is enhanced in a dose-dependent way by a factor isolated from porcine spleen after limited proteolysis. The factor is not dialyzable and is stable for 5 min at 100 degrees C and at pH 2 at room temperature. Trypsin or collagenase treatment does not affect its biological activity. The stimulation of cell growth is independent of serum concentration in the culture medium but is accelerated by insulin supplement. The biochemical properties of this factor indicate a novel growth promotor different from other growth factors.

Animals↗
PubMed1990

Regression estimation from grouped observations applied to structurally diverse D2-receptor antagonists.

Four groups of chemically diverse dopamine D2-receptor antagonists with different group size were studied. The total sample consisted of a mixture of distributions with some influential observations, and the grouped data were characterized by different dispersion matrices but equal mean vectors. Thus, it was difficult to employ pattern recognition approaches. Design repair was used to develop a quantitative structure-activity relationship equation. It was shown that the inhibition constant of all groups depends on the true lipophilic constant.

Animals↗
PubMed1990

Obscure phenomena in statistical analysis of quantitative structure-activity relationships. Part 1: Multicollinearity of physicochemical descriptors.

Multicollinearity of physicochemical descriptors leads to serious consequences in quantitative structure-activity relationship (QSAR) analysis, such as incorrect estimators and test statistics of regression coefficients of the ordinary least-squares (OLS) model applied usually to QSARs. Beside the diagnosis of the known simple collinearity, principal component regression analysis (PCRA) also allows the diagnosis of various types of multicollinearity. Only if the absolute values of PCRA estimators are order statistics that decrease monotonically, the effects of multicollinearity can be circumvented. Otherwise, obscure phenomena may be observed, such as good data recognition but low predictive model power of a QSAR model.

Chemical Phenomena↗
PubMed1990

[Perineal tumors in male patients].

Perineal tumors are rare and are malignant primaries in 66% of cases. We treated three patients with perineal tumors. Histological examination led to a diagnosis of benign tumor (lipoma, granulomatous tumor) in two patients and a diagnosis of local perineal metastasis of a malignant nonseminomatous germ cell tumor in the third. After local tumor excision all patients stayed free of disease for 1-3 years. Neither physical nor clinical examination (standard blood parameters, ultrasound, X-ray of pelvis, urethrocystoscopy and rectoscopy) allowed determination before surgery of whether any of these perineal tumors was benign or malignant; percutaneous biopsy was the only way to find this out preoperatively. Radical excision of the perineal tumor is the treatment of choice. If extended dissemination has not taken place, malignant perineal tumors are curable by radical resection in nearly 80% of cases.

Adult↗
PubMed1988

[Design, goals and preliminary results of the first national breast cancer study--the Federal Ministry of Research and Technology study of "small breast cancer"].

In the multicentre trial sponsored by the Federal Ministry for Research and Technology (= BMFT), mastectomy is compared with breast preservation in patients with pT1 NO MO breast cancer. On the basis of that protocol breast preservation can also be performed by smaller institutions thus implementing the health policy programme of effecting new treatment modalities on a broad basis. We can already recognise a substantial improvement in treatment standards applied by the different disciplines in the hospitals involved in the study. Special emphasis is placed on an optimal patient information about the possible treatment modalities involving the patients in the treatment decision. The evaluation of various prognostic factors will result in additional information on the biology of breast cancer. As a consequence, the definition of the group of patients suitable for breast preservation therapy can be clarified further.

Breast Neoplasms↗
PubMed1988

[Immunohistochemical tumor diagnosis in breast cancer--use for assessing the stage and biology of so-called "small breast cancer"].

Immunohistochemical methods assure the discrimination between neoplastic and reactive Lesions. Epithelial and mesenchymal neoplastic processes can absolutely be distinguished by antibodies directed to intermediate filaments. Growth activity of breast cancer can be evaluated using antibodies against epidermal growth factor receptor and Ki-67 proliferative protein. With antibodies directed to nuclear steroid receptor proteins semiquantitative determinations (immunoreactive score = IRS) and evaluation of genotypic heterogeneity are possible and may be helpful for therapeutic decisions.

Antigens, Neoplasm↗
PubMed1982

Organophosphate poisoning in marmosets.

Due to faulty use of a chlorpyriphos-diazinon combination as a pesticide within a laboratory animal house, 19 marmosets of 2 species (Saguinus fuscicollis and Callithrix jacchus) of 98 at risk died from organophosphorous poisoning (19%) mortality). The clinical signs were those of cholinesterase inhibitors. The morphological lesions consisted predominantly of haemorrhages in several organs, particularly the CNS, and of bone marrow necroses.

Animals↗
PubMed1981

Chronic tubulointerstitial nephritis and wasting disease in marmosets (Callithrix jacchus).

Six marmosets (Callithrix jacchus), 20 months to 16 years old, died from a disease characterized by weakness and paralysis of the hind legs, weight lose, anemia and transient diarrhea. The lesions most prominent at necropsy were subacute to chronic tubulointerstitial nephritis, subacute to chronic pancreatitis, and generalized hemosiderosis. Chronic protein deficiency is believed to be the underlying pathogenic mechanism, since the diet contained no more than 15% animal protein during the last two years, and increasing the amount of protein was accompanied by disappearance of the syndrome.

Animals↗
PubMed1975

Some aspects of sexuality and reproduction in groups of captive marmosets (Callitbrix jacchus).

Several aspects of sexual and reproductive behaviour of Callithrix jacchus were studied for almost five years. The most important results are as follows: (1) In artificial groups (AG), heterosexual interactions were observed with varying frequencies between all group members; in the family groups (FG), however only between the parents (a-male and a-female). (2) In general, sexual interactions between two animals in the AG were tolerated by the other group members; in the FG, the offspring tried to disturb all copulations between the parents. (3) Pre- and postcopulatory play, and copulation with intromission and ejaculation are limited to the highest ranking group members. No difference was found between the highest and lower ranking males in the duration of copulation and number of pelvic thrusts. (4) The breeding privilege is correlated with rankorder; that is, only the most dominant male and female reproduce. This gives the impression of a monogamous relationship between the a-animals. (5) Independent of follicle maturation and ovulation, the a-females indicate estrous characterized by a considerable increase in willingness to copulate, associative behaviour between the a-male and the a-female (both most likely influenced or caused by pheromone activity), as well as aggressive behaviour between the a-female and the lower ranking females in the AG. Conflicts between the dominant and lower ranking group members, especially the females in AG, usually cause the group to split. The ability of the lower ranking females to reproduce is adversely affected by the stress to which they are subjected. (6) The parents of the FG are extremely tolerant of their sexually mature adult sons and daughters; expulsion of offspringe by the parents has not occurred as yet in the FG. (7) There is no certain evidence of cyclic visible changes of the genital region or menstrual bleeding in C. jacchus. (8) Captive C. jacchus females breed all year long; gestation is 144--146 days. (9) Homo- and autosexuality is reported for the males of the FG.

Animals↗
PubMed1975

[Observations, analyses and experiments on the use of the hand in Callithrix jacchus Erxleben, 1777. Part II. Fine analysis of prehensive behavior].

The use of the hand of the common marmoset (Callithrix jacchus Erxleben, 1777) was analysed on the basis of observations and experiments conducted on 56 animals of all age. During arboreal locomotion Callithrix jacchus makes use of the hands for support and for grasping to maintain its balance, The palmar region serves as the main means of support, while the findgers in the locomotor grip also contribute to maintaining balance and support. The greater the need for maintaining balance, for example, during vertikal climbing or in movement along the narow branches, the mre complete the djustment of the hand form on the surface and the more complete the alignment of the hand on the branch will be. Additional support is supplied by the longitudinal palm furrow, caused by the radio-ulnar adduction of the hand edges and by the clinging properties of the volar pads...

Age Factors↗
PubMedDate not supplied

Serum IFN-gamma and IL-10 levels are associated with disease progression in non-obese diabetic mice.

BACKGROUND: The goal of the present study was to determine whether cytokines in the peripheral blood of naive NOD mice correlate with the disease process and thereby would provide a marker for monitoring disease activity. METHODS: Female NOD mice (5, 10 and 14-16 weeks of age) were investigated in a cross-sectional study. In the group of 14-16-week-old mice, non-diabetic and diabetic mice were analysed as different subgroups. The Th1 cytokine (IFN-gamma) and the Th2 cytokine (IL-10) were quantified in serum by sandwich enzyme-linked immunosorbent assay (ELISA). Pancreatic mRNA for IFN-gamma and IL-10 was determined by reverse transcriptase-polymerase chain reaction (RT-PCR) from the same animals. RESULTS: Serum levels of IFN-gamma were initially low but increased with age in NOD mice, reaching the highest levels at diabetes onset (p<0.002 compared to 10 weeks). A similar rise was noted in IFN-gamma gene expression in pancreatic lesions. In contrast, an early peak of serum IL-10 levels was observed in non-diabetic NOD mice (10 weeks) at a stage where non-destructive insulitis occurs. With increasing age a continuous loss of IL-10 until progression towards diabetes was observed. The pancreatic IL-10 mRNA expression correlated with serum IL-10 changes. As a consequence, the ratio of IFN-gamma/IL-10, reflecting the Th1/Th2 balance in the serum, was significantly increased in diabetic compared to non-diabetic NOD mice (p<0.005). CONCLUSION: These results demonstrate, for the first time, that an increased Th2 pattern in the non-diabetic stage preceding a Th1 shift is associated with the development of diabetes in naive NOD mice. Serum cytokines correlate with disease progression and pancreatic cytokine expression during prediabetes. Soluble cytokines measured in the periphery are therefore promising surrogate markers of diabetes development.

Animals↗
PubMedDate not supplied

Intervention in autoimmune diabetes by targeting the gut immune system.

BB rats and nonobese diabetic (NOD) mice spontaneously develop autoimmune insulin dependent diabetes and serve as models for human type I diabetes. During progression of the disease the cytokine pattern elaborated by islet infiltrating immune cells shifts from a Th2 or Th0 toward Th1 type. Only the latter is associated with "destructive" insulitis. We discuss here attempts to modulate disease progression by targeting the gut immune system with bacterial immunostimulants. Oral dosing of diabetes prone BB rats with lipopolysaccharide (LPS) or the Escherichia coli extract OM-89 lead to a Th2-shift of pancreatic mRNA expression. In vitro studies showed that repeated exposure toward LPS or OM-89 lead to downregulation of proinflammatory macrophage responses. In the NOD mouse, repeated oral dosing of OM-89 caused a Th2 shift in the gut cytokine gene expression, probably because of desensitization of macrophages and other antigen presenting cells. Concomitantly, diabetes prevention by oral insulin was improved. In conclusion, oral dosing with bacterial immunostimulants dampens Th1 type immune reactivities of the gut immune system and thereby promotes oral tolerance mechanisms. Downregulation of proinflammatory immune reactivities by repeated exposure to bacterial stimulants requires intact desensitization mechanisms in macrophages or other antigen presenting cells.

Adjuvants, Immunologic↗