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Biomedical subjects

H Rubio

Publications and source records attributed to H Rubio.

18 recordsLinked to original sources
PubMed1991

Production of platelet-derived growth factor by human lung cancer.

The production of platelet-derived growth factor (PDGF) was studied in small cell lung carcinoma, lung squamous carcinoma and lung adenocarcinoma cell lines, and in seven human lung tissues obtained from each type of lung cancer. By indirect immunofluorescence, PDGF was detected in all the cell lines. Likewise, five out of seven biopsies derived from patients with adenocarcinoma and squamous carcinoma, and four out of seven specimens with small cell carcinoma displayed a positive pattern for PDGF. In addition, lung carcinoma cell lines expressed both PDGF-A and PDGF-B/sis genes, as judged by Northern blot analysis. Biologically active, serum-free conditioned media obtained from all three cell lines stimulated the incorporation of [3H]thymidine into quiescent BALB/c-3T3 cells. This effect was abolished when IgG-PDGF antiserum was used. These findings suggest that an abnormal expression of PDGF occurs in the three more frequent types of lung cancer, which can play a potential role in neoplastic transformation and uncontrolled cell growth.

Adenocarcinoma↗
PubMed1991

Adenosine deaminase in the diagnosis of tuberculous pleural effusions. A report of 218 patients and review of the literature.

The activity of adenosine deaminase in the pleural fluid of 218 consecutive patients was studied. According to the etiology of exudative pleural effusions, the patients were divided into the following five groups: (1) tuberculosis; (2) lung cancer; (3) pneumonias; (4) miscellaneous; and (5) idiopathic. Patients with pleural tuberculosis presented significantly higher ADA activity than patients with nontuberculous pleural effusions (p less than 0.0001). The results indicated that in a population with a relatively high prevalence of tuberculosis, the analysis of ADA levels in pleural effusions constitutes a useful marker for the diagnosis which, in addition, can be made quickly and cheaply. Additionally, a comprehensive review of the literature on the role of ADA in the diagnosis of tuberculous pleural effusions is presented.

Adenosine Deaminase↗
PubMed1989

Tuberculosis of the larynx: clinical aspects in 19 patients.

Laryngeal tuberculosis continues to exist in spite of extensive tuberculosis eradication campaigns. We present 19 cases of laryngeal tuberculosis seen in our institution between 1982 and 1987. From the clinical findings it is clear that the localization of the lesions has changed since the preantibiotic era. Today the spread appears to be mainly via lymphatic and hematogenic routes, as opposed to the direct spread more common previously. The fibrosis caused by the lesions is incapacitating and frequently requires surgical correction.

Adolescent↗
PubMed1987

[Misoprostol and cimetidine in the treatment of active duodenal ulcer. Multicenter, double-blind, clinico-endoscopic study. The Argentine experience].

Ten Argentine centers participated in this cooperative trial. One hundred and sixteen patients with endoscopically proven duodenal ulcer entered the study and were randomly assigned to receive either Misoprostol 800 micrograms or Cimetidine 1200 mg, q.i.d. per day, during four weeks of treatment. Patients were allowed to take Amphogel tablets for relief of ulcer pain when necessary, and clinical, lab-tests and adverse effects were evaluated weekly. Ninety-nine patients (Misoprostol 54, Cimetidine 45) completed the study and were evaluable. Success/failure criteria were based on endoscopically documented complete healing at the end of treatment period. Healing rate of four week was 85.2 per cent (46/54) for Misoprostol and 75.5 per cent (34/45) for Cimetidine, with no statistically significant difference. It is concluded that Misoprostol and Cimetidine are highly effective and safety in the healing of active duodenal ulcer. Misoprostol is a new therapeutical alternative for the treatment of duodenal ulcer patients.

Adolescent↗
PubMed1985

Increase of unidentified HLA antigens in pulmonary tuberculosis.

We studied the HLA-A, B,C and DR antigens in 50 patients with advanced pulmonary tuberculosis in an effort to detect some genetic factors which may predispose to the development of this disease. We found a decrease in HLA-A3, A9, B5, B8, B13, B17 and B27. The incidence of "double blank" antigens in the locus A was 18% (2.5% in controls, p less than 0.0005) in the locus B 16% (1.5% in controls, p less than 0.0005) and 28% in the DR locus (7% in controls, p less than 0.0005). Our results suggest that the cells responsible for the immune response in patients with tuberculosis appear to have a diminished number of HLA antigens serologically determined. That phenomenon could be explained by several hypothesis like poor expression of these proteins or masking for another substance. In spite of the mechanism, this abnormal membrane expression of HLA antigens could be important for the development of the disease since the cell-cell interactions critical for the mounting of an adequate immune response might not be appropriate.

Female↗
PubMed1985

[Simple-blind clinical study with sublingual lorazepam vs. injectable diazepam as premedication in colonoscopies].

Those patients premedicated at least 90 minutes before coloscopy, with sublingual lorazepam, showed improved sedative effect, better tolerance and collaboration? Less discomfort and pain when air was, as usually insuflated, as compared with patients receiving intramuscular diazepam. Doctor's opinion coincided with the before mentioned preference for sublingual lorazepam prescribed at least 90 minutes before the endoscopic procedure. No adverse side effects were pointed out.

Administration, Oral↗
PubMed1981

[Percutaneous transhepatic biliary drainage in obstructive jaundice].

PTC was performed in 86 patients with obstructive jaundice, between February/80--March/81 diagnosing 20 cases of the hepatic hilium carcinoma, 14 of pancreatic carcinoma, and 2 multiplex abscess of the liver. PTC-D was successfully attempted on 16 patients, catheterizing the intrahepatic biliary tree in 15 and maintaining a good biliary flow in 10 of them. The catheter was on the correct position into the biliary tree in 6 patients, and the drainage continued for 7-20 days. General improvement was obtained in 83.33%, itching decreased in 40% and disappeared in 60%, cholestasis was reduced in 100% and sepsis in 75%. Complications of the technique were: pain during the introduction of the guide wire (18.75%) and transitory hemobilia (31.21%). PTC-D seems to be a procedure with a precisely indication in every transitory obstructive jaundice, in order to put the patient in better conditions to a definitive therapy: 1) Surgery 2) Prosthesis 3) External-internal biliary drainage.

Aged↗
PubMed1980

[Primary hydatic cyst of the tail of the pancreas. Segmental portal hypertension].

An uncommon purulent primitive hydatic cyst of the pancreatic tail with segmental portal hypertension and gastric varix is reported. Recognition of gastric varix on angiography and immunological diagnosis of hydatidosis clarified the ethiology of hematemesis. Surgical resection of the spleen and pancreas tail cured the patient's recurrent hemorrhages.

Adolescent↗
PubMedDate not supplied

Molecular analysis of p53 tumor-suppressor gene and microsatellites in preneoplastic and neoplastic lesions of the colon and esophagus.

Mutations in exons 4-8 of the p53 gene by the PCR-SSCP analysis in preneoplastic and neoplastic lesions of the colon (n=11) and esophagus (n=18) were screened. p53 overexpression by immunohistochemistry in 11 colonic lesions and 13 microsatellites, in all the patients (n=29), were also studied. A positive result concordancy between the three techniques was found in 1 adenoma and 2 adenocarcinomas of the colon, each with loss of heterozygocity of microsatellites. Metaplastic lesions of esophagus showed biallelic mutations and low frequency of microsatellite alterations. The relationship between genetic alterations in p53, microsatellites and type of colon and esophageal lesions is discussed.

Adenocarcinoma↗
PubMedDate not supplied

Propranolol-induced hyperreactivity in guinea-pig lung parenchyma strips: possible role of histamine.

In vivo and in vitro studies have demonstrated that beta-adrenoceptor blockers enhance the airway smooth muscle contraction to antigen, but the mechanisms are not fully understood. In the present work, we corroborated that propranolol (3.9 x 10(-6) M, 30 min incubation) induces hyperreactivity to antigenic challenge (cumulative concentration-response curve to ovalbumin, 0.01 to 100 micrograms/ml) in lung parenchyma strips from sensitized guinea-pigs. This hyperreactivity was enhanced by indomethacin (3.2 x 10(-5) M) and was unaffected by the lipoxygenase/cyclooxygenase inhibitor phenidone (1 x 10(-4) M). However, the histamine H1 receptor antagonist pyrilamine (1 x 10(-6) M) reduced the potentiation effect of propranolol. These results suggest that bronchoconstrictor prostaglandins, thromboxane A2 and leukotrienes, are not involved in the propranolol-induced lung parenchyma strips hyperreactivity to antigen in vitro, and that histamine may account, at least in part, for such propranolol effect.

Animals↗