[Bibidobacterium cystitis].
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Biomedical subjects
Publications and source records attributed to H S Hieng.
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Esterase B of Escherichia coli has been purified 56 fold with recovery of 39%. The apparent molecular weight as determined by gel filtration was approximately 57000. The pI as determined by isoelectric focusing was 4.6. This enzyme exhibited Michaelis-Menton kinetics with apparent Km of 0.25 mM for l-naphtyl acetate. It remained stable at 60 degrees C but was sensitive to pH values below 6. The esterase activity was completely inhibited by Di-isopropyl-fluorophosphate (DFP) but was resistant to iodoacetamide and to EDTA.
The increase, during a 5-year period (1975-79), in staphylococcal infections resistant to gentamicin and tobramycin has been evaluated in various units of a large Parisian hospital. Out of 3,788 infections, 539 were due to staphylococci that were resistant to gentamicin, tobramycin and kanamycin (GKTo phenotype). All isolates were methicillin-resistant strains, and 53% belonged to serotype 18. Depending upon individual hospital units two patterns of resistance were observed: a progressive one extending to 1979, with an annual average of 9% resistant strains, and a rapid one, which culminated in 1978 with 34% resistant strains.