Biomedical subjects
H S Schneider
Publications and source records attributed to H S Schneider.
Clinical observation utilizing morphine sulfate and hydroxyzine pamoate for sedating apprehensive children for dental procedures: a nine-year report.
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Specific anti-influenza virus antibody production in vitro by lymphocytes from a subset of patients with hypogammaglobulinemia.
Specific anti-influenza virus antibody production in vitro was studied in peripheral blood mononuclear cells from 17 patients with hypogammaglobulinemia. Cells obtained from 6 of 12 patients with common variable hypogammaglobulinemia produced anti-influenza virus antibody, predominantly of the IgM isotype, when cultured in vitro with type A influenza virus. No antibody was produced in vitro, however, by cells from either of two patients with Bruton's type X-linked hypogammaglobulinemia or by cells from any of three patients with X-linked hypogammaglobulinemia and isolated growth hormone deficiency. These studies demonstrate that peripheral blood mononuclear cells from a subset of patients with common variable hypogammaglobulinemia retain the potential to produce specific antibody in response to antigenic stimulation.
Human T-cell subset requirements for the production of specific anti-influenza virus antibody in vitro.
Studies were undertaken to define the helper T-cell requirements for in vitro specific antibody production to influenza virus. Subpopulations of human T cells were separated on the basis of their reactivity with the monoclonal antibody OKT4. B cells cultured with OKT4+ T cells produced specific antibody to influenza virus, while B cells cultured with OKT4- T cells did not. Irradiation (1200 rads) of the OKT4- subset to potentially eliminate suppressor-cell activity did not augment the helper-cell function of that subset. Thus, unlike the cytotoxic T-cell response to influenza, help for an in vitro antibody response is mediated only by OKT4+ T cells.
Influence of intensive embryo collection on subsequent embryo production.
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Influence of day of collection on recovery rate in mature cycling mares.
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Specific anti-influenza virus antibody production in vitro by human peripheral blood mononuclear cells.
Cultures of human peripheral blood mononuclear cells stimulated in vitro with influenza virus were found to produce anti-influenza virus antibody. An enzyme-linked immunosorbent assay was used to measure cumulative antibody secretion into culture supernatants. Optimal antibody production was obtained with 2 x 10(6) peripheral blood mononuclear cells cultured in flat-bottomed wells for 12 days in 2 ml of RPMI 1640 medium supplemented with 10% fetal calf serum, antibiotics, and 4 mM L-glutamine. Antibody could be detected in cultures stimulated with live A/Hong Kong/68 (H3N2), purified formalin-inactivated A/Aichi/68 (H3N2), or live B/Hong Kong/68 influenza virus. Antibody of both IgG and IgM isotypes was produced. Under optimum conditions, the secreted IgG antibody was found to be directed preferentially at antigens on the stimulating virus. Antibody production was shown to require the cooperative interaction of B and T cells. Poisson analysis of B cell precursor frequency showed that approximately 1 in 40,000 B cells could be induced to produce IgG anti-influenza virus antibody in virus-stimulated cultures. Further investigation of this in vitro response should allow dissection of the cellular mechanisms involved in the regulation of antibody production in man.
[Side effects of a single large dose of laxative].
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Prevention is the best treatment.
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Parental education leads to preventive dental treatment for patients under the age of four.
Records from a pediatric dentist's practice, for selected periods of time over a twenty-three-year span (1966-1988), were reviewed to determine the number of patients under four years of age seen for initial dental visits and the reason for the first visit. Reasons given by parents seeking dental care for their children were categorized thus: preventive services, oral injuries, dental caries, and "other" conditions. A parental education system that stressed the benefits of a home-care program and early dental visits resulted in a 36.7 percent increase in children under the age of four seen for preventive services. Special note was taken of the influence of this program on the parents' decision to seek early preventive care for younger siblings of patients already in the practice.