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H S Taper

Publications and source records attributed to H S Taper.

63 records · Page 4Linked to original sources
PubMedDate not supplied

Oligofructose modulates lipid metabolism alterations induced by a fat-rich diet in rats.

The aim of this study was to test the hypothesis that non-digestible fructans, namely oligofructose (OFS), are able to decrease post-prandial lipaemia in rats fed a high-fat diet composed of 10% lard, 4% corn oil and 0.15% cholesterol. Male Wistar rats were divided into three groups: group 1 was fed a standard low-fat diet (AO4), group 2 received the high-fat diet (HF) and group 3 received 10% (w/w) OFS in the HF diet (HF-OFS) for 3 weeks. The OFS supplementation reduced post-prandial triglyceridaemia by more than 50%, compared to both AO4 and HF groups. It also protected rats against the increase in free cholesterol serum level induced by the HF diet. The OFS did not prevent the HF-induced hepatic accumulation of triglycerides, phospholipids and cholesterol; however, histochemical analysis showed smaller lipid droplets in the liver of HF-OFS rats as compared to HF rats. Our results suggest that, when given in HF diet, OFS decreases serum triglycerides through an extra-hepatic event, namely by enhancing triglyceride-rich lipoprotein catabolism.

Animals↗
PubMedDate not supplied

Ultrastructural aspects of autoschizis: a new cancer cell death induced by the synergistic action of ascorbate/menadione on human bladder carcinoma cells.

Scanning and transmission electron microscopy were employed to further characterize the cytotoxic effects of a ascorbic acid/menadione (or vitamin C/vitamin K3) combination on a human bladder carcinoma T24 cell line. Following 1-h treatment T24 cells display membrane and mitochondrial defects as well as excision of cytoplasmic fragments that contain no organelles. These continuous self-excisions reduce the cell size. Concomitant, nuclear changes, chromatin disassembly, nucleolar condensation and fragmentation, and decreased nuclear volume lead to cell death via a process similar to karyorrhexis and karyolysis. Because this cell death is achieved through a progressive loss of cytoplasm due to self-morsellation, the authors named this mode of cell death autoschizis (from the Greek autos, self, and schizein, to split, as defined in Scanning. 1998; 20: 564-575). This morphological characterization of autoschizic cell death confirms and extends the authors previous reports and demonstrates that this cell death is distinct from apoptosis.

Ascorbic Acid↗
PubMedDate not supplied

Inhibitory effect of dietary inulin or oligofructose on the development of cancer metastases.

The possible influence of continuous dietary treatment with 15% inulin or oligofructose on the development of lung metastases of a transplantable liver tumor in young male C3H mice was investigated. Microscopical examination demonstrated a distinct inhibitory effect of this dietary treatment on the development of lung metastases of this tumor. There were 59% of mice bearing lung metastases in the control group, 36% in the inulin fed group and 35% in the oligofructose fed group. The total number of lung metastases was 37 in the control group, 18 in inulin fed and 6 in oligofructose fed mice. Several possible, mechanisms hypothetically involved in this astonishing inhibition of the development of lung metastases by dietary treatment with inulin or oligofructose are discussed.

Animals↗
PubMedDate not supplied

Non-toxic sensitization of cancer chemotherapy by combined vitamin C and K3 pretreatment in a mouse tumor resistant to oncovin.

The effects of combined vitamin C and K3 i.p. injected 3 hours before i.p. administration of single dose of oncovin, to which the ascites liver tumor in mouse (T.L.T.) was completely resistant, were investigated. This pretreatment sensitized the tumor resistant to oncovin, whereas a separate pretreatment with vitamin C or K3 alone was without any effect. This tumor sensitization to the chemotherapy was completely suppressed by catalase pretreatment, thus indicating that hydrogen peroxide generation with subsequent oxidative stress and its consequences may be involved here. Since this sensitization was without any increased general and organ toxicity, its possible introduction into classical protocols of human cancer treatment would be without any supplementary risk.

Animals↗
PubMedDate not supplied

Effects of sodium ascorbate (vitamin C) and 2-methyl-1,4-naphthoquinone (vitamin K3) treatment on human tumor cell growth in vitro. II. Synergism with combined chemotherapy action.

The growth inhibitory effects of a combined application of sodium ascorbate (Vitamin C) and 2-methyl-1,4-naphthoquinone (Vitamin K3) together with various chemotherapeutic agents has been examined on in vitro cultured human endometrial adenocarcinoma (AN3CA) cells. Combined vitamin treatment and chemotherapy in well defined conditions of cell confluence and at the dose levels applied result in a synergistic effect on growth inhibition. The combined vitamins when reaching their own synergistic cytotoxicity levels frequently obscure the additional synergistic effects attributable to the chemotherapeutic agents. Apart from the specific cytotoxic characteristics of the chemotherapeutic drugs examined, the formation of reactive oxygen radicals during treatment, possibly accentuated by less defined secondary mechanisms, appears essentially responsible for the observed stimulated cytotoxicity.

Adenocarcinoma↗
PubMedDate not supplied

Potentiation of radiotherapy by nontoxic pretreatment with combined vitamins C and K3 in mice bearing solid transplantable tumor.

BACKGROUND: The effect of intraperitoneal and oral pretreatment with combined vitamins C and K3 on the single dose radiotherapy of a transplantable solid mouse tumor have been investigated. MATERIALS AND METHODS: Groups of mice bearing intramuscularly transplanted liver tumors, were orally and parenterally pretreated with combined vitamins C and K3 and locally irradiated with single doses of 20, 30, or 40 Gy of X-rays. After this treatment tumor dimensions were measured twice weekly and the approximate tumor volume in groups of pretreated vitamins and irradiated mice was compared to the groups of mice only irradiated and to the absolute control groups without any therapy. RESULTS: This nontoxic pretreatment produced statistically significant potentiation of radiotherapy induced by 20 to 40 Gy of X-rays doses in groups of 11 to 20 mice. Combined vitamins C with K3 most probably constitute a redox-cycling system producing hydrogen peroxide and other active oxygen species to which cancer cells are selectively sensitive due to their frequent deficiency in enzymatic defense system against free oxyradicals agression. CONCLUSIONS: A possible introduction of such nontoxic and selective potentiation procedure into classical protocols of human cancer therapy appears to be generally accessible and without any additional risk for patients.

Animals↗
PubMedDate not supplied

Inhibition effect of dietary inulin and oligofructose on the growth of transplantable mouse tumor.

BACKGROUND: The influence of 15% inulin or oligofructose incorporated in to the basal diet on the growth of transplantable mouse tumor (TLT) was investigated. MATERIAL AND METHODS: This dietary treatment was performed starting at day 7 before tumor transplantation and continued until the end of observation. The results were evaluated by the mortality rates in the ascitic form of tumor, or by twice weekly solid tumor measurements, with vernier caliper. Mortality rates in ascitic tumors and mean solid tumor surface in these experimental groups was compared with those of animals from control groups fed basal diet without supplementary beta (2-1) fructans. RESULTS: The growth of both forms of transplantable mouse tumors was significantly inhibited by the supplementation of the diet with inulin or oligofructose. CONCLUSION: Such a nontoxic dietary treatment appears to be easily used ad without any risk for patients, and is applicable as an adjuvant factor to classical protocols of human cancer therapy.

Analysis of Variance↗