PubMed Health⌕ Search

Biomedical subjects

H SHELDON

Publications and source records attributed to H SHELDON.

At least 19 recordsLinked to original sources

Studies on cartilage. III. The occurrence of collagen within vacuoles of the golgi apparatus.

Electron microscopic observations on chondrocytes from rabbit ear cartilage 72 hours after papain has been given intravenously show that many vacuoles in the Golgi apparatus contain a material with a speckled appearance. Some Golgi vacuoles also contain a material with a banded pattern which can be identified as a collagen. The significance of this observation is discussed.

Animals↗

Studies on cartilage: electron microscope observations on normal rabbit ear cartilage.

Normal rabbit ear cartilage studied with the light and electron microscope shows chondrocytes in which large lipide spherules, and an abundance of glycogen, a few small mitochondria, and relatively few elements of the endoplasmic reticulum can be identified. The chondrocytes contain, in addition, a material which stains strongly with acid fuchsin and appears in the electron microscope as a relatively dense felt-work. In electron micrographs, the matrix of normal rabbit ear cartilage consists of two components: a uniformly distributed moderately dense substance which appears as a fine meshwork without any particular pattern extending from cartilage cell border to cartilage cell border; and a three-dimensional anastomotic network of more dense material, which is best described as "felt-like" lying between the cells. The similarity between the felt-like material of the matrix and the elastic fibers described in previous electron microscope observations is discussed.

Animals↗

An electron microscope study of the epithelium in the normal mature and immature mouse cornea.

1. An electron microscope study at high resolution of the corneal epithelium of the normal mature and immature mouse revealed new information regarding the submicroscopic appearance of these cells. 2. Two thin dense lines separated by a less dense area constituted the structure of the limiting surface membrane of epithelial cells; the thickness of this membrane was about 80 A. 3. Some differences in the appearance of the cytoplasm and mitochondria of cells from the immature mouse cornea and the appearance of the cytoplasm and mitochondria of cells from the adult mouse cornea were observed. 4. The basement membrane appeared as a dense band about 600 A wide separating the basal epithelial cells from the substantia propria. Suggestions of periodicity were seen in some phosphotungstic acid-treated specimens. 5. Round bodies believed to be bacteria were seen on the surface of the outer epithelial cells in the adult mouse cornea but not in the immature, unopened eye.

Animals↗