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Biomedical subjects

H Sachse

Publications and source records attributed to H Sachse.

At least 19 recordsLinked to original sources
PubMed1992

Manufacture and in vitro characterization of a solvent/detergent-treated human plasma.

We have developed a modified solvent/detergent (S/D) treatment to inactivate viruses in human plasma using 1% w/w final concentration of tri(n-butyl) phosphate (TNBP) and Triton X-100 and an incubation period of 4 h at 30 degrees C. The procedure inactivates > or = 10(6) chimpanzee-infectious doses (CID50) of HBV, > or = 10(5) CID50 of HCV, and > or = 10(6.2) tissue culture infectious doses (TCID50) of HIV. After virus inactivation, eleven plasma batches were lyophilized and 12 batches were deep-frozen until further use. The batches were characterized by extensive laboratory tests including measurement of clotting factors I-XIII, von Willebrand factor, plasminogen, inhibitors of blood coagulation and fibrinolysis, and other clinically important plasma proteins. All parameters were determined before and after S/D treatment. Twelve conventional single donor plasma units served as control. There were no marked losses of activities of clotting factors, antithrombin III, protein C, plasminogen, and C1-esterase inhibitor due to treatment. After the S/D step, the levels of these parameters were within the normal range in all batches. The same holds true for total protein, immunoglobulins, albumin, complement factors C3 and C4, haptoglobin, hemopexin, caeruloplasmin, alpha 1-antitrypsin, and pH. Protein S and alpha 2-antiplasmin activities decreased by about 50% and were frequently found to be slightly below the lower limit of the respective normal range after treatment. The interindividual variations of all proteins analysed were significantly lower than in the single donor plasma units. The S/D procedure did not lead to increases of markers indicating activation of hemostasis.(ABSTRACT TRUNCATED AT 250 WORDS)

Antiviral Agents↗
PubMed1987

Molecular cloning and expression of a synthetic DNA coding for the antimicrobial protein of bull seminal plasma.

A DNA carrying the coding sequence for the antimicrobial protein from bull seminal plasma (SAP) was obtained by enzymic ligation of six synthetic oligonucleotides. The 162 bp synthetic DNA fragment was cloned into the C-terminal part of the lacZ-gene employing the vector pUR289. Expression in E. coli in the presence of the inducer isopropylthiogalactoside (IPTG) led to the formation of a fusion protein, which was shown by immuno-blotting to contain immuno-reactive antimicrobial protein. Approximately 90 min after induction, the cells stopped growing and the culture was found to contain no viable cells 3 h after induction. We conclude from this observation that the beta-galactosidase-antimicrobial protein fusion product was toxic for the E. coli cell and that the SAP-residue attached to beta-galactosidase was responsible for the cytotoxicity.

Animals↗
PubMed1985

[Causes and prevention of complications in transurethral interventions. Interventions in the urethra].

The male urethra is extremely vulnerable because of the peculiar morphology. Many urethral lesions and their sequelae are due to the disproportion between the diameter of the urethra and the applied instrument. In order to adjust both calibers it is advisable to use an internal urethrotomy in stead of bougies. Complications which may be caused by catheterisation, and by the treatment of meatal stenosis or urethral strictures and how to avoid them are discussed. In spite of improvements in transurethral equipment and careful transurethral operative technique, perioperative prophylaxis with potent antibiotics, is recommended in order to prevent late complications due to infections.

Catheters, Indwelling↗
PubMed1980

[Transurethral deep desication].

With a special plate electrode introduced through a regular resectoscope it is possible to desiccate tissue in a depth of 6 mm. This "deep desiccation" is used especially in transurethral resection of urothelial tumors of the bladder and in the radical resection of carcinoma of the prostate. The indications for partial and total cystectomy should not be influenced by this procedure. The theoretical basis for the method is described.

Electrodes↗
PubMed1978

[Direct vision cold knife internal urethrotomy (author's transl)].

Direct vision cold knife internal urethrotomy avoids injury to neighboring structures with greater certainty than blind urethrotomy and is possible with all urethral strictures regardless of type or origin. The surgery is also easily performed, usually with local anesthesia, and on an ambulatory basis. The recurrence rate of 12% is relatively low and reoperation is easily performed. An important factor is the postoperative therapy, which requires good patient cooperation.

Female↗
PubMed1976

[Peracetic acid as a disinfectant in the fight against tuberculosis--experimental data-- (author's transl)].

M. tuberculosis is susceptible to peracetic acid as a Disinfectant. However, there is no unanimity how the transmission of Mycobacteriaceae can be prevented and which quantitative requirements are necessary for its action. In practical work protein-like substances and varying numbers of tubercle bacilli are important in the efficacy of sputum disinfection. The tuberculocide effect of peracetic acid is scarcely influenced by such admixtures or by the number of test organisms. A preliminary attempt for killing M. tuberculosis in sputum by means of peracetic acid was successful.

Acetates↗
PubMed1976

[Venous wall graft in Peyronie's disease (author's transl)].

One patient with Peyronie's disease (induratio penis plastica) was treated utilizing plastic surgery to correct the penile curvature. The plaques which invaded the corpora were transversely incised and a section of the greater saphenous vein was sewn into the gap. Postoperatively, the penis is quite straight and the patient has resumed normal sexual intercourse.

Humans↗