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Biomedical subjects

H Saied

Publications and source records attributed to H Saied.

At least 19 recordsLinked to original sources
PubMed2000

Preprotachykinin gene expression in goldfish brain: sexual, seasonal, and postprandial variations.

Recently, we described the complete nucleotide sequence of gamma-preprotachykinin (gamma-PPT) mRNA and the deduced amino acid sequence of the precursor on the basis of molecular cloning and sequence analysis of cDNA from goldfish brain. In the present study, gamma-PPT gene expression in the brain of goldfish was examined using quantitative Northern blot analysis. The results showed that the gamma-PPT gene is highly but differentially expressed in the olfactory bulbs, hypothalamus, and posterior brain regions. There are sexual dimorphism and seasonal variations in gamma-PPT gene expression. In addition, the postprandial changes in gamma-PPT gene expression in the olfactory bulbs and hypothalamus suggest that tachykinin peptides are involved in regulation of feeding behavior in goldfish.

Animals↗
PubMed1999

Postprandial, seasonal and sexual variations in cholecystokinin gene expression in goldfish brain.

Recently, we described the complete nucleotide sequence of cholecystokinin (CCK) mRNA and the deduced amino acid sequence of the precursor on the basis of molecular cloning and sequence analysis of cDNA from goldfish brain. In this study, we investigated the hypothesis that CCK has a role in feeding behavior by examining CCK gene expression in the brain of goldfish using Northern blot. We showed that CCK gene is widely but differentially expressed in broad areas of the goldfish brain, including the olfactory bulbs, telencephalon and preoptic region, hypothalamus, optic tectum-thalamus and posterior brain regions, with highest levels in hypothalamus. We found that CCK mRNA levels in goldfish olfactory bulbs, telencephalon-preoptic region, optic tectum-thalamus, and posterior brain were influenced by sex at least sometime of the seasonal gonadal cycle, with female fish having higher levels than males during at least one of the four seasonal sampling times. We also observed a transient and acute increase in the CCK mRNA levels in the olfactory bulbs, telencephalon-preoptic region, hypothalamus, and posterior brain at 120 min after a meal. These widespread postprandial changes in CCK gene expression in goldfish brain indicate that CCK peptides have multiple roles in regulation of feeding behavior in goldfish. This supports the idea that CCK plays a role as a satiety factor in goldfish.

Animals↗
PubMed1989

[Hydatid cysts in children. Diagnostic and therapeutic aspects. Apropos of 1195 cases].

Hydatid cysts represent a true social problem in Tunisia where 2.1% of rural inhabitants are affected. Hydatid disease is responsible for 10% of the country's surgical activity and costs approximately 800 $ per patient. From April 1967 through January 1987, 1,195 cases of hydatid cyst in children aged 2 to 15 years were treated at the Children's Hospital in Tunis. The involved organ was the lung in 643 cases, the liver in 486 cases, the spleen in 27 cases, and the kidney in 18 cases. With the exception of seven patients with malignant forms, surgery was always performed, with a 1.4% mortality rate and a 2% morbidity rate. We analyze the diagnostic and therapeutic particularities of the various locations of hydatid cyst. Diagnosis rests mainly on ultrasound findings and treatment on conservative procedures as hydatid disease in children carries a good prognosis.

Adolescent↗
PubMed1989

[Gastric duplications in children. Apropos of a case manifested in a newborn infant].

We report a case of gastric duplication with neonatal manifestations. Diagnosis of this infrequent malformation is readily established in most cases upon roentgenologic and ultrasound examinations. Etiopathogenesis is unknown. Therapy is surgical. Complete exeresis involves mutilation because the supernumerary stomach shares the vascular supply to the normal stomach, and because a cleavage plane is lacking. Therefore, incomplete resection should be performed as the condition is benign and good results are consistently achieved with this procedure.

Gastrectomy↗
PubMed1988

[Hydatid cyst of the lung in children. Apropos of 643 cases].

Six hundred forty-three lung hydatic cysts were observed during a 20-year-period (1967-1986) in the Children's Hospital of Tunis. The mean age of the patients was 5 years (2-15 years) and sex ratio 1.6. 91.2% of the patients had a single cyst, 8.8% had multiple cysts. The association of lung and liver localisations was the one most commonly observed (10%). Intra-pleural rupture of the cyst occurred in 3 cases and intra-bronchial rupture (vomica) in 5 cases. 653 thoracotomies were performed for 710 cystectomies, 3 lobectomies and one pneumonectomy. The results are excellent with a 3% post-surgical morbidity and a 0.5% mortality. Nevertheless, hydatic disease is a serious public health problem in endemic areas.

Adolescent↗
PubMed1988

[Peptic complications of gastroesophageal reflux in children. Apropos of 62 cases].

This paper describes 62 cases of peptic complications due to gastro-oesophageal reflux in infants and children at the Children's Hospital in Tunis over a 20 years period. The age of the patients ranged from 6 months to 14 years with a majority of males. Failure to thrive, anemia and respiratory complications were the most common features. Upper gastro-intestinal series and endoscopy were performed in all patients and showed the presence of associated hiatus hernia in 53 patients, oesophagitis in 62 and oesophageal stricture in 33. All patients had a primary course of medical treatment. Fifty-three patients required Nissen's fundoplication. Thirty-one patients required oesophageal dilatation. A complete cure of the structures was achieved in all patients but 7. In the whole series, 4 children died.

Adolescent↗
PubMed1987

[Diagnostic and therapeutic strategy of bilateral nephroblastoma (apropos of 4 cases)].

Four cases of bilateral nephroblastoma out of a total number of 99 cases have been observed during a period of 18 years at the INSE of Tunis (4%). The frequency of associated anomalies, the familial incidence, the young age of the patients are underlined. The main therapeutic modalities are discussed. Based on the most recent studies published in the literature, the prognosis of bilateral nephroblastoma has been improved in recent years with a possibility of survival at 2 years exceeding two thirds of the cases.

Abnormalities, Multiple↗
PubMed1987

[Hereditary nature of nephroblastoma. Apropos of a case of 3 siblings].

Three nephroblastomas, including one bilateral tumour, have been detected in three children belonging to the same family and born from a consanguinous marriage. The clinical and diagnostic particularities compared to non familial "sporadic" nephroblastomas are studied. The pathogenesis with the incidence of chromosomal anomalies, the diagnosis and the frequency of bilateral diseases along with the associated deformities and a non invasive therapeutic attitude are discussed.

Child, Preschool↗
PubMed1987

[Bilateral neuroblastoma. Study of 5 cases and review of the literature].

Five cases of bilateral nephroblastoma out of total of 99 cases have been observed during a period of 18 years at the Children's Hospital of Tunis (5%). The frequency of associated anomalies, the familial incidence, the young age of the patients are underlined. The main therapeutic modalities and prognosis are envisaged.

Antineoplastic Combined Chemotherapy Protocols↗