Biomedical subjects
Publications and source records attributed to H Sawahara.
Carboxyfluorescein (CF) and Calcein (CAL) have almost the same fluorescent spectrum. However, only CAL forms a nonfluorescent chelate with the Co2+ ion. It was proven that this phenomenon can be used for the fractional determination of CF and CAL. And this fractional determination method was available for a study on liposomes.
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The effect of environmental temperature on the content of cytochrome P-450 or the activity of nicotinamide adenine dinucleotide phosphate-cytochrome c (NADPH-cytochrome c) reductase was examined by using microsomes prepared from rats kept at 30 degrees C or 15 degrees C for 14 d. The warm exposure (30 degrees C for 14 d) resulted in a significant reduction of the activity of NADPH-cytochrome c reductase though the content of cytochrome P-450 was not changed. The level of thyroxine and 3,3',5-triiodothyronine was reduced by the warm exposure. From the present observation, it was suggested that the effect of environmental temperature on the metabolism of drugs was due to the activity of NADPH-cytochrome c reductase mediated by the level of thyroid hormone.