Gene expression of protein kinase C subtypes in normal and psoriatic epidermis.
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Epidermis↗
Biomedical subjects
H Schaarschmidt
Publications and source records attributed to H Schaarschmidt.
Dermal cylindroma. Expression of intermediate filaments, epithelial and neuroectodermal antigens.
We report on immunohistochemical staining patterns in so-called apocrine tumors of skin with special emphasis on the dermal cylindroma. The results were compared with apocrine tubular adenoma, syringocystadenoma papilliferum and the normal eccrine sweat gland. A relationship of dermal cylindroma to the apocrine gland is suggested by expression of lysozyme and alpha 1-antichymotrypsin. The tumor shares keratin, epithelial membrane antigen (EMA) and EGF-receptor expression with eccrine and apocrine glands. The presence of intermingled cells with a coexpression of keratin and vimentin argues for a partial myoepithelia-like differentiation. Neuroectodermal antigens are missing. Therefore, dermal cylindroma is classified as an adnexal tumor of skin with a variable rate of cells of apocrine secretory, myoepithelial and undifferentiated phenotypes.
Adenoma↗
[Monosymptomatic Melkersson-Rosenthal syndrome with subsequent vulvitis and perivulvitis granulomatosa].
We report on an 40-year-old woman with granulomatous facial swelling followed by vulvitis and perivulvitis granulomatosa. The characteristic histopathology with epitheloid cell granulomas was seen in samples from both the face and the vulva. By immunohistochemistry the lesions were characterized as consisting mainly of histiocytic and CD-30-positive T cells. The possible aetiology is discussed briefly.
Adult↗
[Nevus spongiosus et albus mucosae].
An 8-year-old girl with a non-familial case of white sponge nevus (WSN) is presented. The differential diagnosis is discussed with reference to anamnestic, clinical and histopathological data. In keratin expression, WSN resembles the epithelia of the hard palate and the tongue.
Child↗
[Hutchinson-Gilford syndrome].
A case report on a 6-year-old boy suffering from the extremely rare Hutchinson-Gilford syndrome (progeria) is presented. The results of histopathological and immunohistological examination of the scar-like skin lesions are reported. Subcutaneous amorphous nodules were eosinophilic, PAS- und elastica-negative und remained unstained with antibodies against collagen type IV, vimentin, and collagenase. The dense perivascular infiltration consisted of CD4+, CD8-, alpha-1-antichymotrypsin-, MAC 387-, and some vimentin-positive cells. Perinodular blood vessels were more abundant and had a thickened wall. Collagen bundles were swollen. The epidermis appeared atrophic with focal basal cell degeneration.
Adipose Tissue↗
[Hidrotic ectodermal dysplasia syndrome--trichooculodermatovertebral syndrome].
We report on a 23-year-old women suffering from a hidrotic ectodermal dysplasia with baldness, xerodermia, kyphosis of the chest, hypopigmented mamillae, disturbances of the menstrual cycle, dysphonia and keratitis punctata superficialis recidivans. The complex condition is classified as a tricho-oculo-dermo-vertebral syndrome. Immunohistological findings suggest a combined alteration of epithelial differentiation of hair follicles and interfollicular epidermis.
Adult↗
[Unilateral systematic linear porokeratosis. Immuno- and lectin histochemistry].
An uncommon case of unilateral systemic linear porokeratosis in a women aged 22 years is reported in this paper. The results obtained from frozen-section investigations into lectin binding were indicative of reduced epidermal fucosylation and sialinization beneath the cornoid lamella. The basal stratum failed to react with a polyclonal antibody against calmodulin. Epidermal reaction, with AS-6 staining against urokinase, was of higher intensity. The above findings, as a whole, are likely to suggest accelerated keratinocyte migration to the stratum corneum in cases of linear porokeratosis and and should actually support assignment of the latter to the group of dermatoses with transepidermal elimination.
Adult↗
Pachyonychia congenita. Immunohistologic findings.
Pachyonychia congenita (PC) is a very rare hereditary disorder of keratinization. Immunohistological findings has so far been lacking. Reported in this paper is a case of Jadassohn-Lewandowsky type PC in a woman aged 18 years on which immunohistological investigations could be performed. Several monoclonal antibodies to filaggrin and keratin were used to stain tissue sections of lesional plantar skin, with a view to studying impairment of epidermal differentiation. While staining patterns comparable to those of normal skin were exhibited by anti-filaggrin and some antikeratins (RPN 1161, A51-B/H4), substantially altered immunostaining was recordable from other anti-keratins. Only superficial vital keratinocytes were stained by RKSE 60 against keratin 10 and K 8.12 against keratins 13 and 16. The authors, in other words, obtained information on expression of keratin 10, normally occurring in all suprabasal keratinocytes, as well as of the basal proliferation keratin 16 in the uppermost vital cell positions of PC lesion. The above results are likely to suggest impairment of keratin expression in cases of PC.
Adolescent↗
[Hyperpigmentation of the face].
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Humans↗
Human eccrine sweat glands--a histochemical approach.
Histogenesis of eccrine sweat glands is incompletely understood. Histochemistry of the human eccrine sweat gland of adult skin by the use of lectins as well as antibodies to neuroglandular antigen (NGA) and urokinase was in favour of a relative independent differentiation from interfollicular epidermis. Expression of NGA by sweat glands is a feature unique among skin appendages. The possible impact of our findings for sweat gland histogenesis is briefly discussed.
Antigens↗
[Nevoid bundle hairs].
We report on two cases of naevoid bundle hair of the scalp. The clinical picture is characterized by an abnormal grouping of hairs emerging from one follicle ostium. The hairs arise from separate follicles but fuse into a common outer hair root sheath in the above the sebaceous gland. The outer hair root sheath is characterized by its abnormal reactivity with monoclonal antibodies against keratin but normal reactivity with anti-filaggrin. Naevoid bundle hair is thought to be an atavistic hair growth pattern.
Antibodies, Monoclonal↗
Immunolocalization of cytokeratins in human eccrine sweat glands.
Eccrine sweat glands of adult human skin were described in terms of immunohistological distribution of cytokeratins using monoclonal antibodies. The results are in favour of a segmental cytokeratin expression and provide a feasible basis for the investigation of pathological conditions.
Adult↗
[Chondroid hidradenoma. Immunohistologic detection of cytokeratin and calmodulin].
The chondroid hidradenoma (chondroid syringoma, mixed tumor of skin) is a rare benign tumor with eccrine differentiation. It is composed of both adenoid structures surrounded by a mucoid stroma and "chondroid" single cells. Tumor cells with immunoreactive calmodulin were found predominantly in the centre of adenoid structures, whereas cells expressing cytokeratin were preferable located as the margins.
Adenoma, Sweat Gland↗
[Pachyonychia congenita. Presentation of a case and histological-histochemical findings].
We report on two cases of pachyonychia congenita, Jadassohn-Lewandowsky type, in a father and daughter. Histopathological examination revealed thickened, aggregated bundles of tonofilaments and an increased number of atypical keratohyalin granula, which is suggestive of an altered keratinization. Immunohistological staining with antibodies to cytokeratins (A45-B/B3, A51-B/H4, A53-B/A2, RPN 1161) was unchanged. Filaggrin could not be detected. Basal cells immunoreactive for calmodulin were markedly reduced or even absent in the rete ridges. Staining with a monoclonal antibody against Ki67 made epidermal cell hyperproliferation seem unlikely. The epidermal lectin binding was normal. C3 was detectable in vessel walls mainly of the stratum reticulare. The findings are discussed with reference to pachyonychia pathogenesis.
Adolescent↗
[Immunohistochemical detection of calmodulin. A contribution to the histogenetic classification of eccrine poroma and clear cell acanthoma].
In previous immunohistochemical studies calmodulin (CaM) was detected in the sweat duct. In further investigations we searched for CaM-reactivity in eccrine poromas and clear cell acanthomas. CaM was only detectable in whorl-like areas, so confirming the concept of a ductal origin of this tumor. CaM-reactivity was failing in both, intraepidermal and malignant eccrine poroma. Acanthomas with clear cell pattern failed to give uniform results, suggesting a heterogenous origin of clear cells in the tumours investigated.
Adenoma, Sweat Gland↗
[Acquired acral fibrokeratoma].
A 33-year-old man with an acral fibrokeratoma of the finger. Clinical, anamnestic, histopathologic and immunohistological findings suggest a traumatically induced connective tissue hyperproliferation with secondary epidermal hyperplasia in this benign fibroepithelial tumour.
Adult↗
[Acrosyringial keratinocytes--no abnormal differentiation in psoriasis vulgaris].
Histochemical investigations were performed in normal and psoriatic epidermis to evaluate possible differences in the acrosyringial reactivity. Luminal (abundant in glycoconjugates, immunoreactive) and outer (almost nonreactive) epithelial cells could be distinguished. Psoriasis did not affect the staining pattern. The findings argue against a primary involvement of acrosyringial keratinocytes in the abnormal differentiation pathway of psoriatic epidermal cells. Moreover, they underline the relative independence of the acrosyringium from interfollicular epidermis.
Biopsy↗
[A combination of psoriasis vulgaris and nevus cell nevus--clinical, histopathologic and histochemical findings].
In a study on 76 patients suffering from psoriasis, we found melanocytic nevi (MCN) within psoriatic lesions in 7%, and in perilesional areas of about 2 cm in 13% of the patients. Under magnification (operating microscope), we failed to detect any signs of psoriasis in both the epidermis covering the MCN and the adjacent epidermis. Histopathologically, 6 out of 7 MCN examined did not show any psoriatic alterations of the epidermis, and in all the 7 cases, the adjacent epidermis was free of psoriasis. Using unfixed frozen sections in histochemistry, we studied the lectin binding of FITC-labeled ConA and UEA I in 5 MCN. The epidermal reaction was comparable to that of psoriatic lesions. In contrast to psoriatic lesions, there was no staining of the spinous layer with a polyclonal antiserum against calmodulin, but only the staining of basal cells as in non-lesional psoriasis. We discuss possible 'protective' factors against psoriasis.
Cell Division↗