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H Sedlmeier

Publications and source records attributed to H Sedlmeier.

16 recordsLinked to original sources
PubMed1994

[The efficiency of photometric determination of skatole in slaughtered swine].

In a group of 264 boars, including both cryptorchides and hermaphrodites, skatole was measured using a photometric method developed in Denmark. It resulted in a concentration of skatole equivalents (SE) in the fat of 0.14 ppm in belly tissue and 0.18 ppm in flomen tissue on average, respectively. 10.6% of belly and 16.3% of flomen tissue exceeded the limiting value of 0.25 ppm skatole for boar taint. The photometric determination of skatole is based on a relatively unspecific reaction according to Ehrlich. Therefore, other derivatives of indole concerning the decomposition of tryptophan are recorded, and the results must be declared as SE. In comparison with HPLC, the photometric method yields values that are too high, indole is always found with skatole in a considerable amount, and the other skatole equivalents seem to play a subordinate part.

Adipose Tissue↗
PubMed1994

[The nutritive value of carp (Cyprinus carpio)].

From January 1991 to February 1992 the composition of filleted carps was investigated. Special attention was given to a possible correlation to origin and season. The gutted, fresh fish were weighted and only the fillets were used for the investigation. All samples were analysed in duplicate by official methods. The results showed that the protein concentration ranged between 13.0 and 21.9%, the lipids content ranged between 0.3 and 23.9%, moisture ranged between 59.8 and 84.2%, and ash content ranged between 0.0 and 1.6%.

Animals↗
PubMed1990

Prolonged radioprotective activity of WR-2721 linked to dextran.

The radioprotective agent WR-2721 was linked to dextran and poly(glutamic acid) respectively, to obtain a prolonged radioprotective ability. Male mice were administered these water soluble polymer conjugates one to 72 hours prior to a whole body irradiation with X-rays. A prolongation of radioprotective efficiency was achieved with two dextran-(WR-2721)-conjugates. For a period of 24 hours between administration, and irradiation dose reduction factors of 1.14 +/- 0.04 and 1.10 +/- 0.03 respectively were found. After 72 hours, no protective effect was observed. Histopathological investigations of the liver for formation of tumors 200 to 600 days after irradiation seems to indicate that a protective effect is not produced by the dextran-(WR-2721)-conjugates.

Amifostine↗
PubMed1986

The influence of the solvent on the toxicity and radioprotective effect of thiophosphate WR2721 in mice.

The influence of various solvents on the toxicity as well as the protective efficiency of thiophosphate WR2721 in X-irradiated male mice is described. The use of Sörensen buffer as solvent with a pH-value of 7.4 leads to a statistically significant lower lethality in animals than aqua bidest. However, the radiation efficacy of thiophosphate with respect to the acute lethality within 30 days after irradiation is not influenced by the choice of the solvent used. A dose reduction factor of 2.49 +/- 0.03 is gained for the LD50/30 d at 500mg WR2721 per kg animal weight.

Amifostine↗
PubMed1985

Purification and some properties of an acryloyl-CoA reductase of Clostridium kluyveri.

Acryloyl-CoA reductase, a presumably previously unknown soluble enzyme, is present in Clostridium kluyveri. It catalyses the reduction of the carbon-carbon double bond of acryloyl-CoA or ethyl vinyl ketone and other alpha, beta-unsaturated carbonyl compounds at the expense of reduced methylviologen. On the basis of a Vmax/Km ratio, which is at least 18 times higher than that for the next best substrate (E)-2-butenoyl-CoA, the enzyme is called acryloyl-CoA reductase. A purity of over 90% was achieved. The apparent molecular mass, as determined by gel chromatography, is 28.4 kDa. Dodecyl sulfate gel electrophoresis shows subunits with a molecular mass of 14.2 kDa. Based on a molecular mass of 28.4 kDa about 1.5 mol FMN have been observed. Less than 0.2 g-atom iron per mol protein were determined. Ferredoxin or flavodoxin seem to be able to carry electrons from hydrogenase to the acryloyl-CoA reductase. The addition of hydrogen to the alpha-carbon of ethyl vinyl ketone occurs from the re-side.

Chromatography, DEAE-Cellulose↗
PubMed1983

Experimental chemotherapy of radiation injury with synthetic lysophospholipid analogs in mice.

Synthetic lysophospholipids represent a variety of analogs of the naturally occurring 2-lysophosphatidylcholine. Some of these compounds showed significant therapeutic effects on the survival of mice following radiation injury when administered after various doses of whole-body X irradiation. Such therapeutic effects were discernible even when the treatment was given 6 hr after irradiation, and both intravenous and oral application were effective. Intravenous application of 2 X 25 mg/kg lysophospholipid after whole-body X irradiation around the LD50 resulted in significantly higher numbers of surviving animals. The mode of action remains speculative.

Animals↗
PubMed1981

[Efficacy of protective effect of aminopropylamino-ethylthiophosphate in neutron beam, gamma or X-irradiation of mice (author's transl)].

The efficacy of the radioprotective agent aminopropylamino-ethylthiophosphate (WR 2721) was tested against different kinds of radiation on female NMRI-mice. The animals were divided into 4 groups and were exposed to neutrons as main component, to secondary gamma rays in a tissue-equivalent phantom, to pure gamma rays and to X-rays respectively. The following dose reduction factors have been obtained: 1.05 for neutron-irradiation, 1.33 for gamma irradiation in tissue-equivalent phantom, 1.69 for pure gamma rays and 2.08 for X-rays. The protective agent WR 2721 is apparently more efficient against low LET radiation. The mechanism of protection has been discussed in this respect.

Amifostine↗
PubMed1980

[Investigations on combined injuries, 29th communication. Protective effect of aminopropylamino-ethylthiophosphate (WR 2721) in mice with radiation and combined injuries (irradiation and skin wound or burn) (author's transl)].

Because of the crucial practical importance of combined injuries, investigations of the efficacy of the radioprotective agent aminopropylamino-ethylthiophosphate (WR 2721) were performed on mice which were whole-body X-irradiated and stressed with a second trauma either as an open wound or as burn wounds on the skin. In cases of sole whole-body irradiation, probit analyses of our data yielded the dose reduction factors (DRF) of 2.42 (NMRI-Hannover) and 2.12 (NMRI-Kisslegg). In case of a combination of an open skin wound (SW) with irradiation in mice of the Hannover breed the following dose reduction factors have been obtained: for irradiation+SDW 10 min p. r. the DRF 2.22, for irradiation+SW 2 d p. r. the DRF 2.30, and for irradiation+SW 8 d p. R.. the DRF 2.29. In cases of combined injuries with two burn wounds (BW) as second trauma in mice of the Kissblegg breed yielded: the DRF 2.01 for irradiation+BW 10 min p. r., and the DRF 1.91 for irradiation+BW 2 d p. r. The protective effect of WR 2721 is apparently similar in the various groups with combined injuries as compared to the animals exposed to whole-body irradiation only. The mechanisms of action of the sulphur-containing radioprotective agents are discussed.

Amifostine↗
PubMed1980

[Investigations with beagles about toxicity and radioprotective effect of the clinical radioprotection substance WR 2721. Part I. Toxicity of WR 2721 (author's transl)].

The toxicity of the chemical radioprotection substance WR 2721 (S-2-(3-aminopropylamino)ethyl-thiophosphate) was examined in 25 beagles. The study showed that the toxicity of the substance increases as the dose gets higher. Between the doses 200 and 250 mg/kg of body weight, the increase of toxicity was significantly greater than could be expected on the basis of the dose difference. Until a dose of 200 mg/kg, the authors found no side effects which would have disturbed vital functions, but higher doses led to marked symptoms of intoxication.

Amifostine↗
PubMed1979

[Investigations on combined injuries, 24th communication: response of blood circulation and respiratory function in mice upon whom was inflicted whole-body irradiation in combination with open skin wounds (author's transl)].

Combined injuries were inflicted upon NMRI-mice, hurting each with an open skin wound subsequent to sublethal exposition to X-rays. Lethality among animals with combined injuries was between 40 and 60%, while animals only irradiated and those with only a skin wound had lethalities between 10 and 20% and 0%, respectively. Blood circulation and respiration of animals injured with combined lesions were studied in an attempt to understand the cause of the high lethality in this group. The blood volume and the oxygen transport capacity were drastically reduced in animals with combined injuries as compared to those in animals only irradiated, although plasma volume, vascular permeability and distribution of the blood volume remained similar in both groups. Analyses of gases and acid/base composition of blood revealed neither respiratory nor metabolic acidosis. These findings indicate that combined injuries hardly impair blood circulation and respiratory function.

Acid-Base Equilibrium↗
PubMed1979

[Investigations on combined injuries, 25th communication: renal function and energy metabolism in mice with injuries combined of whole-body irradiation and an open skin wound (author's transl)].

In NMRI-mice injured with combined lesions (whole-body irradiation with 500 R and, two or seven days later, an open skin wound), lethality increased from 10 or 20% following sole irradiation up to 60%. Investigations of urinary excretion and serum levels in animals with combined injuries did not show undue elevations of the levels of electrolytes, creatinine, urea, uric acid and acid or alkaline equivalents. Analysis of lactate, pyruvate, ATP and ADP in liver tissue and blood serum indicated no significant changes in the energy metabolism in animals with combined injuries in comparison with mice irradiated only. These findings and those of our last communication (24. Mitteilung) seem to be incompatible with the presence of a shock state as cause of the high lethality of mice with combined injuries.

Animals↗