H Seidell | PubMed Health

PubMed1984

Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities.

A computer program has been designed to aid development of synthetic strategies for oligonucleotides produced by solid-phase chemical techniques. The program reduces the time required to develop a strategy and a data file from hours to minutes. The program contains inventories, provides cost analyses, and generates and stores other associated data. The program searches an inventory of sequences for that sequence to avoid duplicate synthesis. If the sequence is not in the inventory the program devises a synthetic strategy, calculates the amounts of reagents and labor costs necessary to complete the synthetic oligonucleotide. The program also deducts the reagents from inventory files. Physical data is also calculated. A file is generated in a sequence inventory for storage of the data as well as other data that will be generated during the purification processes. All variable parameters can be easily edited. The programs were designed to provide a cross-referencing feature for data analysis and can use several parameters as a constant.

Base Sequence↗

PubMed1984

Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities.

A computer program has been designed to aid development of synthetic strategies for oligonucleotides produced by solid-phase chemical techniques. The program reduces the time required to develop a strategy and a data file from hours to minutes. The program contains inventories, provides cost analyses, and generates and stores other associated data. The program searches an inventory of sequences for that sequence to avoid duplicate synthesis. If the sequence is not in the inventory the program devises a synthetic strategy, calculates the amounts of reagents and labor costs necessary to complete the synthetic oligonucleotide. The program also deducts the reagents from inventory files. Physical data is also calculated. A file is generated in a sequence inventory for storage of the data as well as other data that will be generated during the purification processes. All variable parameters can be easily edited. The programs were designed to provide a cross-referencing feature for data analysis and can use several parameters as a constant.

Computers↗

PubMedDate not supplied

The evaluation of synthetic strategies for oligonucleotides of defined sequence. Computer programs in nucleic acid synthesis: I.

A computer program has been developed to aid in the evaluation of strategies for the synthesis of oligodeoxyribonucleotides of defined sequence. The program reduces the time required for the development of the most cost-effective strategy from several hours to a few minutes. The chemist supplies the program with the order of internucleotide bond formation, the final amount of material desired and the data giving anticipated reaction yields and molar ratios of reactants. The Mr and amount required for each intermediate in the proposed synthetic approach is then calculated. All of this data can be stored, edited or printed. By changing various input parameters (e.g., order of internucleotide bond formation, expected reaction yield), the chemist can utilize this program to analyze multiple synthetic approaches rapidly. The synthetic approach that will minimize the amounts of the expensive starting materials needed to complete the synthesis of the desired DNA can thus be quickly identified.

Base Sequence↗