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H Soboll

Publications and source records attributed to H Soboll.

5 recordsLinked to original sources
PubMed1981

Oncogenicity of heat-inactivated simian adenovirus SA7.

Simian adenovirus SA7 heated for 30 minutes at 70 degrees C retained part of its ability to induce tumors in newborn hamsters. Tumors were also induced by DNA extracted from the heated virus. However, neither residual replicating, i.e. cytopathogenic, nor transforming and T-antigen-inducing activities could be detected in vitro in CV-1 and hamster embryo cells inoculated with the heated virus. The results are discussed in terms of a possible release of viral nucleic acid at the high temperature. They also show that current tests for effective virus inactivation based exclusively on the recognition of replicating virus should be reevaluated.

Adenoviridae↗
PubMed1978

Morphological revertants of adenovirus type 12-transformed hamster cells.

Morphological revertants have been isolated from one line of adenovirus type 12-transformed hamster cells. This line, T637, is oncogenic in hamsters and contains multiple copies of the virus genome per cell. Different parts of the virus genome are represented in non-stoichiometric amounts and the virus DNA persists in the cells in an integrated form. The pattern of integrated virus genomes has been determined by the blotting technique. In the T637 line, morphological revertants arise spontaneously at relatively high frequency. Two of these revertants have been cloned. In contrast to the T637 line, the revertants F10 and G12 exhibit fibroblastic morphology. The patterns of integrated virus genomes in the revertants differs markedly from that of the T637 line; one of the revertant cell lines, F10, appears to have lost all virus DNA sequences. The morphological revertants continue to express the oncogenic phenotype, although the time required to produce tumours in animals appears to be prolonged compared to the parental BHK21 and the T637 cell lines. A number of biological parameters of the revertant lines have also been investigated.

Adenoviridae↗