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Biomedical subjects

H Soliman

Publications and source records attributed to H Soliman.

49 records · Page 3Linked to original sources
PubMed1988

Plant proteins in milk replacers for rearing buffalo calves. I. Effect of replacing half of the milk proteins by plant proteins on the preweaning performance of buffalo calves.

In an experiment, 12 female and 8 male buffalo calves aged 3 to 4 weeks with an average of 65.2 kg live body weight were divided into 4 equal groups. Group 1 received dried skim milk plus non-milk fat. In groups 2, 3, and 4, 50% of the milk protein were replaced by American soybean flour, Egyptian soya meal, or corn glutine. Scouring occurred in all groups during the first three weeks. Death losses occurred in group 2 (2 calves) and 4 (1 calf). During the first three experimental weeks the calves consumed on average 828, 868, 847, 696 g dry matter (DM) as liquids. The average daily gain (ADG) was 229, 215, 252, 48 g/d, respectively. The energy consumption reached 4.1, 4.6, 3.8, 16.6 TDN/kg ADG. During the second period, the calves consumed 1.57, 1.45, 1.55, 1.65 kg DM as liquid and solid feedstuff. Up to a live body weight of 90 kg they had a daily increase of 695, 611, 593, 600 g. The energy used amounted to 1.98, 2.08, 2.28, 2.40 TDN/kg ADG. The apparent digestibility of the crude protein was 95, 92, 91, 92% during the first period and 81, 77, 76, 73% during the second period.

Animal Feed↗
PubMed1987

Laser Doppler velocimetry in critically ill patients.

Cutaneous blood flow may be an indirect measure of circulatory function estimated by continuous, noninvasive laser Doppler velocimetry (LDV). It has been postulated that LDV may be a useful monitor of cardiac output changes. To test this hypothesis, LDV was evaluated in 67 critically ill adult patients with simultaneous measurements of cardiac index (CI) and other physiologic variables. Heated and unheated laser probes were utilized. The results indicate that LDV reflects acute directional changes of CI. Further, with the heated laser probe, the magnitude of acute CI change is reflected. However, there are gradual changes over time of LDV which may occur independently of CI or other monitored variables. Hence, absolute LDV values are not predictive of absolute CI values.

Adult↗
PubMed1987

[Blood phenolsulfotransferase and monoamine oxidase-B activity in essential arterial hypertension].

Circulating phenolsulfotransferase M and P and monoamine oxidase activities were determined in 18 untreated essential hypertensive patients and in 35 normotensive healthy controls. Phenolsulfotransferase M is involved in the sulfoconjugation of catecholamines and their metabolites while PST P preferentially sulfates phenolic substrates. After lysis of whole blood, enzymatic activities were determined by radioenzymatic techniques using as substrates 3-methoxy-4-phenoxyphenylglycol for PST M, phenol for PST P and [14C] beta-phenylethylamine for MAO. Blood MAO activity measured by this method is fully accounted for by platelet MAO-B activity. Concerning blood PST activities, no significant difference was found in hypertensive patients compared to normotensive controls (PST M: 1.69 +/- 0.17 versus 1.66 +/- 0.08 nmoles of MHPG-sulfate/ml of blood/hour; PST P: 0.36 +/- 0.05 versus 0.27 +/- 0.04 nmoles of phenol-sulfate/ml of blood/hour). MAO activity was higher in women than in men. Significantly lower MAO B activities were observed in hypertensive patients both in men (19.25 +/- 2.20, n = 8 versus 24.35 +/- 2.22, n = 14, desaminated beta-phenyl-ethylamine/10(9) platelets/hour, x +/- SEM, p less than 0.05) and in women (23.92 +/- 2.74, n = 10 versus 35.76 +/- 2.35, n = 21, p less than 0.01) when compared to normotensive controls of the same sex. Recent in vitro studies have suggested that a reduction in platelet MAO B activity may be induced by an alteration in the phospholipidic and/or calcium environment of the enzyme. Low MAO activity in other tissues such as liver or vascular endothelium could contribute to the high sympathetic tone observed in these patients.

Arylsulfotransferase↗
PubMed1986

Serum inorganic sulphate: quantitation by a new radiochemical method.

A new procedure for serum inorganic sulphate determination is described. The method is simple, accurate and highly reproducible. It is based on radioisotopic dilution of 35S-sulphate by protein-free, phosphate-free serum. The decrease in initial 35S-sulphate specific activity (corresponding to added serum sulphate) is determined in the supernatant after partial precipitation of sulphate by barium ion. Serum sulphate is computed thereafter from a standard curve. Recovery of added sulphate from dialysed or undialysed serum was 100%. The intra- and interassay coefficients of variation were 3.5% and 4.1%, respectively. The serum sulphate concentration measured by this method in 93 normal subjects was 400 +/- 90 mumol/l (mean +/- SD), which agreed with the values reported in the literature. Serum sulphate did not correlate with sex (p greater than 0.40, n = 93), but a significant correlation with age was observed (r = 0.25, p less than 0.02, n = 93).

Adolescent↗
PubMed1980

Dihydroxybenzene derivatives of antimicrobial activity.

For possible antimicrobial activity, catechol-3,5-disulphonyl-chloride resercinol-4,5-disulphonylchloride and resorcinol-2,4,6-trisulphonylchloride were condensed with amines, phenols, 8-hydroxyquinolin and hydrazine hydrate.

Anti-Bacterial Agents↗
PubMedDate not supplied

Plasma 21-deoxycortisol: comparison of a time-resolved fluoroimmunoassay using a biotinylated tracer with a radioimmunossay using (125)iodine.

Plasma 21-deoxycortisol (21DF) is an excellent marker of 21-hydroxylase deficiency. Currently, it is the only marker able to detect heterozygous carriers with 21-hydroxylase deficiency after ACTH stimulation. We have already developed radioimmunoassays for 21DF using first tritiated, then 125I-21DF which had a ten-fold higher sensitivity. However, because the lifespan of 125I-21DF is short, the tracer needs to be reprepared every two months and this multiplies the risk of contamination by radioactive 125I vapours. We therefore developed a non-isotopic 21DF assay that uses a 21DF-biotin conjugate with a original bridge, a diaminopropyl arm, linking the steroid to biotin. The 21DF-biotin conjugate was measured by time-resolved fluorescence after adding streptavidin-europium to the microtitration wells. The analytical qualities of this assay were very similar to those of the radioimmunoassay using 125I-21DF as tracer. The results obtained by the two methods, in either normal subjects or patients with 21-hydroxylase deficiency, were virtually the same.

Adrenal Hyperplasia, Congenital↗