Two isomeric icosatrienoic acids with double bonds at positions 5,8,14 and 5,11,14 and their 1-14C- and acetylenic analogues were prepared. The syntheses and characterization of these acids are described.

8,11,14-Eicosatrienoic Acid↗

PubMedDate not supplied

Polyunsaturated fatty acid biosynthesis: a microsomal-peroxisomal process.

The synthesis of 22-carbon fatty acids, with their first double bond at position 4, requires the participation of enzymes in both peroxisomes and the endoplasmic reticulum as well as the controlled movement of fatty acids between these two cellular compartments. It has been observed that there is generally an inverse relationship between rates of peroxisomal beta-oxidation vs those for the microsomal esterification of fatty acids into 1-acyl-sn-glycero-3-phosphocholine. With a variety of different substrates it was found that when a fatty acid is produced in peroxisomes, with its first double bond at position 4, its preferred metabolic fate is to move to microsomes for esterification rather than to serve as a substrate for continued degradation. The required movement, and the associated reactions, in peroxisomes and microsomes is not restricted to the synthesis of 4,7,10,13,16-docosapentaenoic acid and 4,7,10,13,16,19-docosahexaenoic acid. When microsomes and peroxisomes were incubated with NAD, NADPH and malonyl-CoA it was found that 6,9,12-octadecatrienoic acid was metabolized to linoleate. Collectively our findings suggest that there may be considerably more recycling of fatty acids between peroxisomes and the endoplasmic reticulum than was previously recognized.

Animals↗

PubMedDate not supplied

The roles of anabolic and catabolic reactions in the synthesis and recycling of polyunsaturated fatty acids.

Generally the biosynthesis and degradation of compounds take place in separate subcellular compartments. The synthesis of 22 carbon acids, with their first double bond at position 4, requires anabolic enzymes in the endoplasmic reticulum as well as peroxisomal beta-oxidation enzymes. Partial degradation-resynthesis cycles, using enzymes in these two subcellular compartments, may play an important role in determining what PUFA are available for membrane lipid biosynthesis.

Endoplasmic Reticulum↗