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Biomedical subjects

H Srinivasa

Publications and source records attributed to H Srinivasa.

17 recordsLinked to original sources
PubMed2005

A rapid densitometric method for simultaneous quantification of gallic acid and ellagic acid in herbal raw materials using HPTLC.

Gallic acid and ellagic acid are two widely occurring phenolic compounds of plant origin, to which many biological activities including anticancer and antiviral activity have been attributed. A simple HPTLC method has been developed for the simultaneous quantification of gallic acid and ellagic acid. The method was validated for precision, repeatability, and accuracy. Instrumental precision was found to be 0.083 and 0.78, and the repeatability of the method was found to be 1.07 and 1.50 (% CV) for gallic acid and ellagic acid, respectively. The accuracy of the method was checked by a recovery study conducted at two different levels and the average percentage recovery was found to be 101.02% for gallic acid and 102.42% for ellagic acid. The above method was used for the quantification of gallic acid and ellagic acid content in seeds of Abrus precatorius Linn., whole plant of Phyllanthus maderaspatensis Linn., and flowers of Nymphaea alba Linn. The proposed HPTLC method for the simultaneous quantification of gallic acid and ellagic acid was found to be simple, precise, specific, sensitive, and accurate and can be used for routine quality control of herbal raw materials and for the quantification of these compounds in plant materials.

Abrus↗
PubMed2000

Human ocular dirofilariasis in Kerala Southern India.

A twenty-six year old female presented to the Ophthalmology OPD with swelling of the upper eyelid. On excision, two long, thin, dead worms were removed. Microscopic and HPE showed the worms to belong to the Dirofilaria species.

Adult↗
PubMed1999

A high incidence of ciprofloxacin resistance in urinary isolates in eastern Nepal.

A study was conducted to examine the incidence of ciprofloxacin resistance in the strains of bacteria isolated from patients with suspected urinary tract infection (UTI) and to analyse the level of resistance. A total of 63 bacterial isolates were grown from quantitative urine culture and were in significant count. Antibiotic susceptibility tests were done by using disc diffusion method of Kirby-Bauer and the level of resistance was analyzed by break point minimum inhibitory concentration (MIC). Majority of bacteria isolated and tested were Gram negative with Escherichia coli as the commonest isolate. Antibiotic susceptibility testing revealed a high prevalence of resistance to nalidixic acid, ampicillin and norfloxacin.

Anti-Infective Agents↗
PubMed1998

Urinary IgG antibody capture particles adherence test for detecting HIV antibodies: a preliminary report.

It is known that antibodies to HIV are secreted in urine and saliva. IgG antibody capture particle adherence test (GACPAT) has been shown to provide accurate results for antibodies in urine. The aim of the study was to study the sensitivity and specificity of this test in our settings. 114 urine samples were collected from both HIV positive and HIV negative patients and were analysed using GACPAT. The test has a sensitivity of 92.3% and specificity of 100%. We feel that this test has a definite role in our country especially for surveillance purposes.

HIV Antibodies↗
PubMed1985

Coagglutination and counter immunoelectrophoresis in the rapid diagnosis of typhoid fever.

The efficacy of two methods--coagglutination (COAG) and counter immunoelectrophoresis (CIE)--in the rapid diagnosis of typhoid fever was studied in parallel with blood and clot cultures on 114 clinically suspected cases. Retrospective analysis showed that only 58 eventually were discharged and had typhoid fever. Antigen detection on their sera was done by both methods, concomitant with antigen detection on culture supernates by CIE. Sera from 50 controls were subjected to both tests. Agglutinating anti-serum being unsatisfactory in the CIE system, anti-serum to the LPS fraction of Salmonella typhi "O" 901 was used in both tests after absorption with Escherichia coli and Salmonella paratyphi A. Analysis of data with reference to retrospectively confirmed typhoid cases show that S. typhi was isolated in 58.6% and 58.3% of blood and clot cultures; antigen detection by CIE in their supernates was 81.1% and 79.2%, respectively. This correlated closely with serum COAG (81.0%) in contrast to serum CIE (5.7%). Thus, COAG was superior to CIE for serology. However, CIE done on culture supernates precludes such tedious procedures as absorption of staphylococcal agglutinins and the confirmatory blocking test.

Agglutination Tests↗
PubMed1983

Seroepidemiology of malaria in Karnataka State: longitudinal study of a population from an area with high incidence at Kolar, South India.

Serologic methods have considerable value in epidemiologic surveillance of malaria. Antigens prepared from P falciparum cultured in vitro have been found to be sensitive and to a certain extent specific in the IFA test to detect malarial antibodies in the sera from a population continuously exposed to P falciparum and Plasmodium vivax infections [1]. However, it is necessary to assess the serologic data against the results of conventional examination of peripheral blood smears in monitoring the effect of antimalaria measures on malaria transmission. With this objective, we studied a randomly selected population from GPHU, which is located in an area in the Kolar District of the state of Karnataka where malaria is known to be endemic. During the transmission period of 1980 (from July to November), the annual parasitic incidence was 202 per 1,000, with 78% due to P vivax and 22% to P falciparum. The antimalaria measures were subsequently intensified by the GPHU staff, which lead to a decrease in the incidence of malaria in 1981 (60 per 1,000, with 91% due to P vivax and 9% to P falciparum; data obtained from GPHU records). Serum samples were collected after each transmission period and tested by IFA using a local isolate of P falciparum as antigen. There was a marked decrease in the number of seropositive samples in the second batch among children less than 10 years of age and a threefold decrease in malarial antibody titers up to the age of 15 years.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗