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Biomedical subjects

H Stadler

Publications and source records attributed to H Stadler.

At least 73 records · Page 4Linked to original sources
PubMed1982

[Shock-treatment in gastro-intestinal-bleeding with 3,5% isocyanate crosslinked polypeptide solution].

In a prospective study infusions of 3,5% isocyanate crosslinked polypeptide solution 500 ml (Haemaccel 3,5%) were given to patients with gastro-intestinal-bleeding for shock treatment and prophylaxis. The increase in intravascular volume was always satisfactory. We did not see any undesirable side effects (anaphylaxis, anaphylactoid reaction, coagulopathy, oliguria).

Gastrointestinal Hemorrhage↗
PubMed1980

Proton NMR detection of acetylcholine status in synaptic vesicles.

The storage granules of cholinergic nerve terminals, the synaptic vesicles, have a central role in the mechanism of cholinergic transmission, and direct evidence as to whether acetylcholine is stored in them in a free or partially immobilized form is therefore of interest. In some secretory systems it has become evident that low molecular weight secretion products are stored within the granules as complexes with proteins or proteoglycans. The application of high resolution NMR spectroscopy to isolated granules has recently elucidated the internal structure of secretory granules. We report here that proton NMR analysis of isolated cholinergic synaptic vesicles from Torpedo marmorata allows the identification of acetylcholine within the organelle. The spectrum shows that all the acetylcholine is dissolved in an essentially fluid phase within the core. Vesicular and uncompartmentalized acetylcholine can be distinguished from each other and in addition, any hydrolysis to choline and acetate can be monitored. The results open the possibility of studying dynamics of acetylcholine pools and their breakdown in cholinergic tissue by a direct and non-perturbing method.

Acetylcholine↗
PubMed1980

[Proof of the jejunogastric bile reflux after BII partial gastrectomy using 99mTc diethyl-IDA (author's transl)].

A history was obtained and barium meal and gastroscopy performed on 42 patients after a Billroth II partial gastrectomy. In order to demonstrate and quantify jejuno-gastric bile reflux, hepato-biliary sequential scintigraphy was carried out using 99mTc diethyl-IDA. The method has advantages over conventional techniques in that it is easy for the patient and demonstrates reflux both when the stomach is empty and full. The result showed that all patients who had evidence of inflammatory changes or ulcers on barium meal or gastroscopy had evidence of reflux.

Adult↗
PubMed1980

[Prophylaxis and therapy of colon perforation in diagnostic fiber colonoscopy (author's transl)].

Three cases of colonic perforation which we could observe in 622 own colonoscopies and two which have been referred to us for surgical repair, are reported. On behalf of these cases the cause of such a perforation as a complication of colonoscopy is pointed out. The symptoms of colonic perforation are not very impressive and somewhat incharacteristic. The treatment of choice is immediate laparotomy, whereby the decision whether simply suture of the defect or resection with or without colostomy remains to be made intraoperatively. In case of massive pneumoperitoneum the puncture of the lower abdomen can be made as an emergency operation.

Adult↗
PubMed1979

Isolation of synaptosomal plasma membranes from cholinergic nerve terminals and a comparison of their proteins with those of synaptic vesicles.

Plasma membranes were purified from purely cholinergic nerve endings (synaptosomes) isolated from the electric organ of Torpedo marmorata. Synaptosomes were lysed, membranes recovered and further separated by density gradient centrifugation. A fraction was obtained enriched in 5'-nucleotidase, Na+, K+-activated ATPase and acetylcholine esterase. Morphological examination showed abundant membrane fragments of the size range of synaptosomes and few of vesicle size. The fraction has a characteristic protein composition upon gel electrophoresis. Five reproducible major bands with apparent Mr of 100000, 75000, 52000, 42000 and 35000--33000 are found. A gel-electrophoretic comparison with proteins from synaptic vesicles from the same source (major bands Mr 160000, 147000, 34000 and 25000) was made. Comigration of major bands was detected in one-dimensional gel electrophoresis with the 42000-Mr, 35000--33000-Mr and 34000-Mr components. Upon two-dimensional gel electrophoresis the 42000-Mr component comigrates with a similar component in vesicles, recently characterized as actin; the other components are different. The presence of tubulin-like polypeptides is unlikely. Beside actin, all major vesicle proteins are often detected in small amounts in the plasma membrane preparation. It cannot be decided if they result from fused or contaminating vesicle membranes, but since they are essentially absent in some preparations, it seems that the plasma membrane does not contain vesicle proteins.

Animals↗
PubMed1978

Chemical composition of cholinergic synaptic vesicles from Torpedo marmorata based on improved purification.

Cholinergic synaptic vesicles from the electric organ of Torpedo marmorata have been purified to a constant composition and a higher transmitter content than previously reported. By optimising the extraction conditions and using a two-step purification on discontinuous and continuous sucrose density gradients, 10-fold higher acetylcholine and ATP values per weight of protein were obtained. The purity of the vesicle preparation was confirmed by electronmicroscopy, absence of marker enzymes, behaviour in density gradient centrifugation, as well as by a specific and reproducible protein composition. Vesicles contain 6.9 mumol acetylcholine and 1.0 mumol ATP per mg protein. The lipid/protein ratio of 3.5 (w/w) indicates a lipid-rich membrane. The value suggests the absence of a proteinaceous core. Upon dodecylsulphate gel electrophoresis a distinct protein pattern is obtained with components ranging from 20000 to 160000 in molecular weight. Vesiculin, reported earlier to be a low-molecular-weight vesicle protein, is not detected. One of the major bands comigrates with muscle actin from the same animal. Further characterisation of this protein by two-dimensional gel electrophoresis suggested that it is an actin-like polypeptide. Evidence for a specific association of this actin-like protein with vesicles and its possible involvement in the neurosecretory process is discussed.

Acetylcholine↗
PubMed1978

The effect of phenylthiocarbamide (PTC) on mouse brain development.

PTC--when dispensed during the whole fetal development--causes a disturbance of brain development in new born mice. This disturbance in manifested by a reduction of the cell number, a reduced protein content and a reduced activity of acetylcholinesterase in the brain.

Acetylcholinesterase↗
PubMed1978

[Gastroscopy after gastric operations (author's transl)].

Gastroscopy after surgery of the stomach gives exact information about functional and morphological alterations. The aim of gastroscopy is to detect complications of the early postoperative period as well as of the later phase. Early diagnosis of gastric carcinoma after partial gastrectomy for benign lesions is only possible by routine gastroscopy.

Gastroscopy↗
PubMed1977

Improved electrophoretic and immunochemical techniques for the identification and characterization of mutant proteins, applied to ribosomal protein S8 in Escherichia coli mutants.

The ribosomal proteins of 11 mutants which are sensitive to starvation at elevated temperature and of 36 transductants derived from them were studied with several electrophoretic, immunochemical and proteinchemical methods. The following results were obtained: (1) Ribosomal protein S8 is altered in three of these mutants. (2) The amino acid exchange in proteins S8 of mutant N4128 is Glu leads to Lys in position 59 of the protein chain. (3) Temperature sensitivity and inability to recover from starvation at elevated temperatures are caused by the same mutational event which is, however, unrelated to the alteration in protein S8. Several electrophoretic and immunological procedures were applied during the characterization of these mutants. A modified immunoelectrophoresis on cellulose acetate gels was developed, and proved to be the most applicable procedure for the detection of mutationally altered ribosomal proteins. This procedure may gain general importance for detecting mutational alterations in other proteins.

Amino Acid Sequence↗
PubMed1976

Determination of the amino-acid sequence of the ribosomal protein S8 of Escherichia coli.

The primary structure of protein S8 from the 30-S ribosomal subunit of Escherichia coli was determined mainly by automatic Edman degradation using a modified Beckman protein sequenator and the solid-phase sequentor of Laursen. The complete sequence, containing 109 amino acids, was derived by analysing peptides from tryptic, chymotryptic, thermolysin, staphylococcal protease and cyanogen bromide digestion of the protein. The amino acid composition was found to be (aspartic acid)6, (asparagine)3, (threonine)5, (serine)5, (glutamic acid )7, (glutamine)6, (proline)5, (glycine)6, (alanine)11, (valine)9, (methionine)4, (isoleucine)7, (leucine)9, (tyrosine)3, (phenylalanine)3, (lysine)11, (arginine)8, (cysteine)1. S8 is a basic protein and binds to the 16-S RNA; knowledge of its sequence is necessary for a detailed study of its interaction with the ribosomal RNA.

Amino Acid Sequence↗
PubMed1975

"In vivo" release of endogenous neurotransmitters in cat limbic regions: effect of chlorpromazine and of electrical stimulation.

The effect of chlorpromazine (10 mg/kg i.v.) on the spontaneous release of endogenous dopamine (DA), noradrenaline (NA) and acetylcholine (ACh) within limbic areas perfused by means of the push-pull cannula was investigated in the gallamine-immobilized cat. Chlorpromazine increased the liberation of DA and NA in the nucleus accumbens septi, indicating blockade of the amine receptors. However, the drug did not change the output of ACh from this nucleus nor from ventral and dorsal hippocampal formations which receive a cholinergic input from the septum as indicated by several published findings and by the increased liberation of ACh after electrical stimulation of the homolateral nucleus medialis septi. These results seem to exclude that cholinergic neurons of some limbic areas mediate the effect of the blockade of DA (and of NA) receptors which is possibly involved in the antipsychotic action of neuroleptic drugs.

Acetylcholine↗